Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,836,073 C→T 7.0% intergenic (‑177/+218) yjeJ ← / ← yjeK hypothetical protein/predicted lysine aminomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,836,0730CT 7.0% 295.1 / 7.9 100intergenic (‑177/+218)yjeJ/yjeKhypothetical protein/predicted lysine aminomutase
Reads supporting (aligned to +/- strand):  ref base C (49/44);  new base T (3/4);  total (52/48)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01

Only 100 of 105 total aligned reads displayed.

CACTCTATATAAACAGAAAAGAAAGGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACT  >  minE/2836004‑2836143
                                                                     |                                                                      
cactcTATATAAACAGAAAAGAAAGGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCa                                                                       >  1:1258441/1‑71 (MQ=255)
cactcTATATAAACAGAAAAGAAAGGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCa                                                                       >  1:2832153/1‑71 (MQ=255)
 actcTATATAAACAGAAAAGAAAGGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCa                                                                       >  1:5533715/1‑70 (MQ=255)
        ataAACAGAAAAGAAAGGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGaat                                                                >  1:3510291/1‑70 (MQ=255)
        ataAACAGAAAAGAAAGGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGaat                                                                >  1:4469510/1‑70 (MQ=255)
         taAACAGAAAAGAAAGGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGaataa                                                              >  1:5276528/1‑71 (MQ=255)
          aaaCAGAAAAGAAAGGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGaataa                                                              >  1:2270090/1‑70 (MQ=255)
          aaaCAGAAAAGAAAGGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGaataa                                                              >  1:1993733/1‑70 (MQ=255)
           aaCAGAAAAGAAAGGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGc                                                                        >  1:1426812/1‑59 (MQ=255)
           aaCAGAAAAGAAAGGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAAtt                                                            <  1:774309/71‑1 (MQ=255)
            aCAGAAAAGAAAGGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAAtt                                                            >  1:1574391/1‑70 (MQ=255)
            aCAGAAAAGAAAGGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTa                                                           <  1:2589002/71‑1 (MQ=255)
                aaaagaaaGGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAgg                                                       <  1:3243840/71‑1 (MQ=255)
                 aaagaaagGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGt                                                      <  1:2547271/71‑1 (MQ=255)
                  aagaaagGTTATTTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGaat                                                                <  1:3601489/60‑1 (MQ=255)
                  aagaaagGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGaat                                                                <  1:2565273/60‑1 (MQ=255)
                  aagaaagGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGaat                                                                <  1:2481809/60‑1 (MQ=255)
                  aagaaagGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGaat                                                                <  1:355885/60‑1 (MQ=255)
                  aagaaagGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGtt                                                     <  1:830894/71‑1 (MQ=255)
                    gaaagGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGaat                                                                <  1:4084055/58‑1 (MQ=255)
                      aagGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGc                                                 <  1:4088794/71‑1 (MQ=255)
                      aagGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGc                                                 <  1:507945/71‑1 (MQ=255)
                      aagGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGc                                                 <  1:4789455/71‑1 (MQ=255)
                       agGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTa                                                    >  1:3650230/1‑67 (MQ=255)
                        ggTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCaa                                               <  1:4198680/71‑1 (MQ=255)
                          ttAATTATTGGTGTTGGTTATATAAAGAGTAACAACAGCAATGTATATGAATAATTAAAGGTTATGCAACg                                             <  1:1978766/71‑1 (MQ=255)
                          ttAATTATTGGTGTTGGTTATATAAAGAGTAACAACAGCAATGTATATGAATAATTAAAGGTTATGCAACg                                             <  1:4255079/71‑1 (MQ=255)
                          ttAATTATTGGTGTTGGTTATATAAAGAGTAACAACAGCAATGTATATGAATAATTAAAGGTTATGCAACg                                             <  1:2313676/71‑1 (MQ=255)
                          ttAATTATTGGTGTTGGTTATATAAAGAGTAACAACAGCAATGTATATGAATAATTAAAGGTTATGCAACg                                             <  1:1225056/71‑1 (MQ=255)
                          ttAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCa                                                                       <  1:1151352/45‑1 (MQ=255)
                           tAATTATTGGTGTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACggg                                           >  1:4407345/1‑71 (MQ=255)
                            aaTTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACggg                                           >  1:5429158/1‑71 (MQ=255)
                             attattGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACggg                                           >  1:789717/1‑70 (MQ=255)
                              ttattGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAACAATTAAAGGt                                                      <  1:4935607/58‑1 (MQ=255)
                               tattGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTAt                                                   <  1:3545912/60‑1 (MQ=255)
                               tattGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTAt                                                   <  1:1893571/60‑1 (MQ=255)
                                attGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTa                                                    >  1:5501061/1‑58 (MQ=255)
                                attGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTa                                                    >  1:5604147/1‑58 (MQ=255)
                                attGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGc                                          >  1:571008/1‑68 (MQ=255)
                                 ttGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCa                                         <  1:4399369/68‑1 (MQ=255)
                                  tGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTa                                                    >  1:522822/1‑56 (MQ=255)
                                  tGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTa                                                    >  1:2214414/1‑56 (MQ=255)
                                  tGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTa                                                    >  1:3926753/1‑56 (MQ=255)
                                  tGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCa                                         >  1:3776892/1‑67 (MQ=255)
                                    gtgtTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTa                                                           <  1:5008315/47‑1 (MQ=255)
                                    gtgtTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTa                                                           <  1:4912924/47‑1 (MQ=255)
                                    gtgtTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTa                                                           <  1:1199169/47‑1 (MQ=255)
                                     tgtTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTa                                  <  1:1901898/71‑1 (MQ=255)
                                     tgtTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTa                                  <  1:2342735/71‑1 (MQ=255)
                                     tgtTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTa                                  <  1:377623/71‑1 (MQ=255)
                                       ttAGCTATATAAAAAGTAACAACAGCAATGCatat                                                                    >  1:20820/1‑35 (MQ=255)
                                       ttAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGtt                                                     <  1:3469715/50‑1 (MQ=255)
                                         aGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTaaa                                                         >  1:954722/1‑44 (MQ=255)
                                         aGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTa                                                    >  1:3285298/1‑49 (MQ=255)
                                         aGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTa                                                    >  1:2414143/1‑49 (MQ=255)
                                         aGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATg                                                  >  1:4882129/1‑51 (MQ=255)
                                         aGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATg                                                  >  1:3707568/1‑51 (MQ=255)
                                         aGCTATATAAAAAGTAACAACAGCAATCCATATGAATAATTAAAGTTTAt                                                   >  1:1867840/1‑50 (MQ=255)
                                           cTATATAAAAAGTAACAACAGCAATGCATATGAATAATTa                                                           <  1:506492/40‑1 (MQ=255)
                                           cTATATAAAAAGTAACAACAGCAATGCATATGAATAATTa                                                           <  1:5498872/40‑1 (MQ=255)
                                             atataAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCaa                                               >  1:3846786/1‑50 (MQ=255)
                                             atataAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCaaa                                       >  1:4188554/1‑58 (MQ=255)
                                             atataAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCaaa                                       >  1:3205297/1‑58 (MQ=255)
                                             atataAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCaaa                                       >  1:3688585/1‑58 (MQ=255)
                                              tataAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTa                                  <  1:1682662/62‑1 (MQ=255)
                                              tataAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTccc                          <  1:2185475/70‑1 (MQ=255)
                                               ataAAAAGTAACAACAGCTATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAg                        >  1:4142390/1‑71 (MQ=255)
                                                taAAGAGTAACAACAGCAATGTATATGAATAATTAAAGGTTATGCaa                                               >  1:3350605/1‑47 (MQ=255)
                                                taAAGAGTAACAACAGCAATGTATATGAATAATTAAAGGTTATGCaa                                               >  1:4990828/1‑47 (MQ=255)
                                                taAAGAGTAACAACAGCAATGTATATGAATAATTAAAGGTTATGCaa                                               >  1:1682448/1‑47 (MQ=255)
                                                taAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGc                                          >  1:585679/1‑52 (MQ=255)
                                                taAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGt                       >  1:671519/1‑71 (MQ=255)
                                                taAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGt                       >  1:2918506/1‑71 (MQ=255)
                                                 aaaaaGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGc                                                 <  1:2522116/44‑1 (MQ=255)
                                                 aaaaaGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGc                                                 <  1:258575/44‑1 (MQ=255)
                                                 aaaaaGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGc                                                 <  1:55071/44‑1 (MQ=255)
                                                 aaaaaGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGaaa                                               >  1:1999067/1‑46 (MQ=255)
                                                  aaaaGTAACAACAGCAATGCATATGAATAATTaaa                                                         >  1:4906364/1‑35 (MQ=255)
                                                  aaaaGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTa                                  <  1:5555261/58‑1 (MQ=255)
                                                    aaGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTaaa                                >  1:97483/1‑58 (MQ=255)
                                                    aaGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTaaa                                >  1:1912364/1‑58 (MQ=255)
                                                     aGTAACAACAGCAATGCATATGAATAATTAAAGGTTAt                                                   >  1:5229342/1‑38 (MQ=255)
                                                     aGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTcc                           >  1:2352787/1‑62 (MQ=255)
                                                     aGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTcc                           >  1:2770578/1‑62 (MQ=255)
                                                     aGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAAt                  <  1:1881314/71‑1 (MQ=255)
                                                       tAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGa                >  1:4587810/1‑71 (MQ=255)
                                                         acaacaGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGa                >  1:1369710/1‑69 (MQ=255)
                                                         acaacaGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGa                >  1:3022634/1‑69 (MQ=255)
                                                         acaacaGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGa                >  1:256440/1‑69 (MQ=255)
                                                         acaacaGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGAc               <  1:167443/70‑1 (MQ=255)
                                                         acaacaGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGAc               <  1:585093/70‑1 (MQ=255)
                                                         acaacaGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGAc               <  1:461364/70‑1 (MQ=255)
                                                          caacaGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAg             <  1:3558010/71‑1 (MQ=255)
                                                              aGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTg         >  1:4186546/1‑71 (MQ=255)
                                                              aGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCACAGTTTAATGACAGGTTg         <  1:2675797/71‑1 (MQ=255)
                                                               gCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGa                >  1:1833756/1‑63 (MQ=255)
                                                                  aTGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCGGTTTAATGACAGGTTGCTGt     <  1:1785796/71‑1 (MQ=255)
                                                                     cATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTaaa                                >  1:4728982/1‑41 (MQ=255)
                                                                     cATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGAc               <  1:759023/58‑1 (MQ=255)
                                                                     cATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACt  >  1:5171136/1‑71 (MQ=255)
                                                                     |                                                                      
CACTCTATATAAACAGAAAAGAAAGGTTAATTATTGGTGTTAGCTATATAAAAAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACT  >  minE/2836004‑2836143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: