Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,870,843 G→T 14.2% intergenic (+86/‑94) rnr → / → rlmB exoribonuclease R, RNase R/23S rRNA (Gm2251)‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,870,8430GT14.2% 161.9 / 9.8 56intergenic (+86/‑94)rnr/rlmBexoribonuclease R, RNase R/23S rRNA (Gm2251)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (37/11);  new base T (2/6);  total (39/17)
Fisher's exact test for biased strand distribution p-value = 7.01e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

AAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAG  >  minE/2870774‑2870910
                                                                     |                                                                   
aagaagAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                    >  1:164125/1‑71 (MQ=255)
    agagGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc                                                                <  1:2947504/71‑1 (MQ=255)
      agGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACa                                                               >  1:2556739/1‑70 (MQ=255)
      agGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAt                                                              <  1:4607426/71‑1 (MQ=255)
          ttAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAAt                                                           >  1:5389853/1‑70 (MQ=255)
          ttAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAAt                                                           >  1:1966575/1‑70 (MQ=255)
          ttAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAAt                                                           >  1:399353/1‑70 (MQ=255)
          ttAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAAt                                                           >  1:4549262/1‑70 (MQ=255)
           tAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCa                                                         >  1:3208322/1‑71 (MQ=255)
                 gCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCaa                                                    <  1:2078551/70‑1 (MQ=255)
                     aCAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAAtt                                                 <  1:4532247/69‑1 (MQ=255)
                     aCAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTc                                                <  1:528715/70‑1 (MQ=255)
                      cAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCaa                                              <  1:737980/71‑1 (MQ=255)
                           tGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAatatat                                         >  1:4884821/1‑71 (MQ=255)
                           tGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAatatat                                         <  1:4211964/71‑1 (MQ=255)
                           tGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAatatat                                         <  1:1370511/71‑1 (MQ=255)
                                  tGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCa                                     >  1:1168042/1‑68 (MQ=255)
                                    ctCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGAt                                <  1:238707/69‑1 (MQ=2)
                                    ctCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATc                               <  1:769773/70‑1 (MQ=2)
                                       aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACg                                                       >  1:4954284/1‑45 (MQ=255)
                                       aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCAt                             >  1:3231049/1‑71 (MQ=255)
                                       aTGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATg                            <  1:3836467/71‑1 (MQ=2)
                                       aTGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATg                            <  1:3973050/71‑1 (MQ=2)
                                        tGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATg                            <  1:705961/70‑1 (MQ=2)
                                        tGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATg                            >  1:3543351/1‑70 (MQ=2)
                                        tGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATg                            <  1:4806689/70‑1 (MQ=2)
                                         gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAatata                                          >  1:1040324/1‑56 (MQ=255)
                                         gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCAt                             <  1:66238/69‑1 (MQ=255)
                                         gCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATg                            >  1:4622790/1‑69 (MQ=2)
                                         gCCAGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAatata                                          >  1:215123/1‑56 (MQ=255)
                                              aTGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCaga                                   >  1:2936130/1‑58 (MQ=255)
                                              aTGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCaga                                   >  1:5315801/1‑58 (MQ=255)
                                              aTGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCaga                                   >  1:5062382/1‑58 (MQ=255)
                                              aTGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGcc                      <  1:2857564/71‑1 (MQ=255)
                                               tGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCaa                                              >  1:3216614/1‑46 (MQ=255)
                                               tGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGAt                                >  1:185567/1‑60 (MQ=255)
                                                gcggcgTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCaa                                              >  1:1504040/1‑45 (MQ=255)
                                                      tGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATa                 >  1:4364611/1‑68 (MQ=255)
                                                        aaCGCCTTATCCGGCCTACATAATCACGCAAATTCaa                                              >  1:2510881/1‑37 (MQ=255)
                                                        aaCGCCTTATCCGGCCTACATAATCACGCAAATTCaa                                              >  1:2024884/1‑37 (MQ=255)
                                                        aaCGCCTTATCCGGCCTACATAATCACGCAAATTCAat                                             >  1:2760744/1‑38 (MQ=255)
                                                        aaCGCCTTATCCGGCCTACATAATCACGCAAATTCAat                                             >  1:5474362/1‑38 (MQ=255)
                                                        aaCGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTa                          >  1:454123/1‑57 (MQ=255)
                                                        aaCGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACg             <  1:2576102/70‑1 (MQ=255)
                                                        aaCGCCTTATCCGGCCTACATAATCACGCAAATTAATATATTGCAGAGATCATGTa                          >  1:2381709/1‑56 (MQ=255)
                                                           gCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGt         >  1:5519933/1‑71 (MQ=255)
                                                              ttATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTa                          >  1:4179547/1‑51 (MQ=255)
                                                                aTCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCAt     >  1:3344522/1‑70 (MQ=255)
                                                                aTCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCAt     >  1:3119327/1‑70 (MQ=255)
                                                                aTCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCAt     >  1:645033/1‑70 (MQ=255)
                                                                aTCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCAt     >  1:3675356/1‑70 (MQ=255)
                                                                  ccGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAg  >  1:285360/1‑71 (MQ=255)
                                                                  ccGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAg  >  1:2819967/1‑71 (MQ=255)
                                                                   cGGCCTACATAATCACGCAAATTCAATATATTGCa                                     >  1:3558496/1‑35 (MQ=255)
                                                                   cGGCCTACATAATCACGCAAATTCAATATATTGCa                                     >  1:3672851/1‑35 (MQ=255)
                                                                   cGGCCTACATAATCACGCAAATTCAATATATTGCa                                     >  1:2983924/1‑35 (MQ=255)
                                                                     |                                                                   
AAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAG  >  minE/2870774‑2870910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: