| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 312,687 | T→C | 27.5% | T62T (ACA→ACG) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 312,687 | 0 | T | C | 27.5% | 134.8 / 58.6 | 91 | T62T (ACA→ACG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (27/39); new base C (8/17); total (35/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGT > minE/312620‑312756 | cggAATCGCTGGTGGAGGACATATACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTc < 1:452269/70‑1 (MQ=255) aGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCAcc < 1:503301/71‑1 (MQ=255) aGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCAcc < 1:4382629/71‑1 (MQ=255) aGTCACTGTTAGAGGACATGTACATCAGGTTATCGACACCGTTCATATTCTGTTCGATAACCTGTGTCAcc < 1:436169/71‑1 (MQ=255) gTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTgtgt < 1:2012146/66‑1 (MQ=255) gTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTgtgt < 1:5364792/66‑1 (MQ=255) tCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCAc < 1:943488/68‑1 (MQ=255) cACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCgtg > 1:1985889/1‑71 (MQ=255) aCTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCgtgt > 1:123631/1‑71 (MQ=255) aCTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCgtgt > 1:4809147/1‑71 (MQ=255) aCTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCgtg < 1:1597281/70‑1 (MQ=255) tGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCgtgt > 1:4639839/1‑69 (MQ=255) ttAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTg > 1:2363662/1‑71 (MQ=255) ttAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTg < 1:3220642/71‑1 (MQ=255) ttAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTg > 1:105193/1‑71 (MQ=255) gtggagGACATATACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTg < 1:3392200/68‑1 (MQ=25) agagGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCa > 1:1133247/1‑71 (MQ=255) gagGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCAc > 1:3497441/1‑71 (MQ=255) gagGACATATACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCg < 1:5062440/61‑1 (MQ=255) ggACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTg < 1:4116180/71‑1 (MQ=255) ggACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTg < 1:277181/71‑1 (MQ=255) aCATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGtt < 1:3322075/71‑1 (MQ=255) cATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt < 1:3734500/71‑1 (MQ=255) cATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt < 1:2185003/71‑1 (MQ=255) aTGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGtttt > 1:1843486/1‑71 (MQ=255) tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCg < 1:4256768/70‑1 (MQ=255) tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCg < 1:4250009/70‑1 (MQ=255) tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCg < 1:3021689/70‑1 (MQ=255) tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGGCCTGCACTGtttt < 1:2766564/68‑1 (MQ=255) tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATc > 1:2500976/1‑56 (MQ=37) tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCa < 1:2033213/61‑1 (MQ=255) tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCa < 1:5588538/61‑1 (MQ=255) cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt < 1:2673768/71‑1 (MQ=255) cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt > 1:35407/1‑71 (MQ=255) cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt < 1:1243728/71‑1 (MQ=255) cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt < 1:1173121/71‑1 (MQ=255) aTCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCg > 1:86618/1‑49 (MQ=255) tCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATc < 1:4368823/70‑1 (MQ=37) cAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGt > 1:1705101/1‑60 (MQ=37) cAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGt > 1:3756695/1‑60 (MQ=37) cAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGt > 1:1745536/1‑60 (MQ=37) aGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTg < 1:5672140/53‑1 (MQ=255) aGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTg < 1:1798601/53‑1 (MQ=255) aGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAg < 1:3614084/70‑1 (MQ=255) aGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAg < 1:5294707/70‑1 (MQ=255) aGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAg < 1:3307584/70‑1 (MQ=255) gTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAgcgc < 1:3808628/71‑1 (MQ=255) ttATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCAGCgtgt < 1:3216678/46‑1 (MQ=255) ttATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCgtgt < 1:2168653/46‑1 (MQ=255) tATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCg > 1:5689768/1‑71 (MQ=255) tATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCg > 1:979089/1‑71 (MQ=255) tATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCg > 1:2702726/1‑71 (MQ=255) aTCGATACCGTTCATATTCTGTTCGATACGCTGTGTCACCGTGTCCTg > 1:3241964/1‑48 (MQ=255) aTCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTg > 1:1435535/1‑48 (MQ=255) aTCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCgt < 1:5697045/42‑1 (MQ=255) aTCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCgt < 1:1473856/42‑1 (MQ=255) aTCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGtc > 1:1652766/1‑54 (MQ=37) cGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCa > 1:248889/1‑59 (MQ=25) agATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg > 1:3245035/2‑71 (MQ=255) gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg < 1:1161302/70‑1 (MQ=255) gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg < 1:5188206/70‑1 (MQ=255) gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg < 1:125038/70‑1 (MQ=255) gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg < 1:269216/70‑1 (MQ=255) gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg < 1:4475126/70‑1 (MQ=255) gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGt < 1:388676/71‑1 (MQ=255) gATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGTTCTGCGCATCAGCGCCcgga < 1:5384541/70‑2 (MQ=255) gATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCcggt < 1:1290593/70‑2 (MQ=255) aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt > 1:45095/1‑52 (MQ=255) aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt > 1:4070017/1‑52 (MQ=255) aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt > 1:2680137/1‑52 (MQ=255) aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg > 1:2838786/1‑69 (MQ=255) aCCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTcc < 1:2491616/40‑1 (MQ=255) aCCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTcc < 1:58486/40‑1 (MQ=255) aCCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTag > 1:1469681/1‑70 (MQ=255) ccGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTc > 1:1595539/1‑51 (MQ=255) cGTTCATATTCTGTTCGATAACCTGCGTCACCgta < 1:2345576/35‑2 (MQ=25) cGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATagttt < 1:3017300/71‑4 (MQ=255) gTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTaggagg > 1:3180705/1‑71 (MQ=255) ttCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGcc < 1:3628965/59‑1 (MQ=255) atatTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCgg < 1:2095546/70‑1 (MQ=255) tatTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATagt < 1:3632931/63‑2 (MQ=255) ttCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCa > 1:4439182/1‑48 (MQ=255) ttCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTagga > 1:3892778/1‑62 (MQ=255) ttCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATc < 1:3153770/47‑1 (MQ=255) tCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATagtt > 1:1346194/1‑59 (MQ=255) cTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATc < 1:180377/70‑1 (MQ=255) cTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCg > 1:2623153/1‑71 (MQ=255) cGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGgag < 1:2043722/62‑1 (MQ=255) cGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAg < 1:1227074/42‑1 (MQ=255) gATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTa > 1:356855/1‑68 (MQ=255) aTAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATag < 1:1605184/51‑1 (MQ=255) aTAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATag < 1:2063184/51‑1 (MQ=255) ccTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGt < 1:3274199/61‑1 (MQ=255) gtgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCcggcgg > 1:2464280/1‑70 (MQ=255) tgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCcggc < 1:758756/67‑1 (MQ=255) tgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGt < 1:3920667/70‑1 (MQ=255) tgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATAGTTACTGCCGGCGGt < 1:4952196/70‑1 (MQ=255) | TGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGT > minE/312620‑312756 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |