Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,872,254 C→A 12.1% L169M (CTG→ATG)  ulaF → L‑ribulose 5‑phosphate 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,872,2540CA12.1% 220.5 / 11.3 74L169M (CTG→ATG) ulaFL‑ribulose 5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (36/29);  new base A (6/3);  total (42/32)
Fisher's exact test for biased strand distribution p-value = 7.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

ACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTG  >  minE/2872189‑2872322
                                                                 |                                                                    
aCGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTgggg                                                                 >  1:4287228/1‑71 (MQ=255)
      ggCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGaaa                                                             <  1:431422/69‑1 (MQ=255)
          aCGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCt                                                       >  1:1056150/1‑71 (MQ=255)
           cGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCt                                                       >  1:412421/1‑70 (MQ=255)
            gCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCa                                                     >  1:3175748/1‑71 (MQ=255)
            gCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCa                                                     >  1:2834798/1‑71 (MQ=255)
            gCCGAGCCGCTGCATACGCCGGCAATTGTGGTGTATCAGCACGGGCCGTTGGCCTGGGGGAAAGATGCTCa                                                     >  1:2461073/1‑71 (MQ=255)
             ccGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCAc                                                    <  1:157381/71‑1 (MQ=255)
             ccGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCAc                                                    <  1:943868/71‑1 (MQ=255)
                 gCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTc                                                      >  1:5437778/1‑65 (MQ=255)
                 gCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATg                                                >  1:2041557/1‑71 (MQ=255)
                       gCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTgggg                                                                 <  1:956092/48‑1 (MQ=255)
                       gCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGaaa                                                             >  1:2585054/1‑52 (MQ=255)
                         aTACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCAt                                        <  1:3823442/71‑1 (MQ=255)
                         aTACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCAt                                        <  1:2226036/71‑1 (MQ=255)
                         aTACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCAt                                        <  1:3846571/71‑1 (MQ=255)
                          tACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCAt                                        >  1:875985/1‑70 (MQ=255)
                              ccGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCt                                                                     >  1:3344338/1‑37 (MQ=255)
                              ccGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCt                                                                     >  1:807407/1‑37 (MQ=255)
                                  gAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATg                                                >  1:22326/1‑54 (MQ=255)
                                  gAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATg                                                >  1:5521392/1‑54 (MQ=255)
                                   aaTTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGaaa                                                             <  1:4718489/40‑1 (MQ=255)
                                     ttGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCggtggt                             >  1:923546/1‑70 (MQ=255)
                                     ttGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCggtggt                             >  1:2306317/1‑70 (MQ=255)
                                       gtggtgTATCAGCACGGGCCGTTCGCCTGGGGGAAAg                                                            >  1:2071874/1‑37 (MQ=255)
                                       gtggtgTATCAGCACGGGCCGTTCGCCTGGGGGAAAg                                                            >  1:3587105/1‑37 (MQ=255)
                                         ggtgTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATg                         <  1:2067678/70‑1 (MQ=255)
                                          gtgtATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATgg                        <  1:2211007/70‑1 (MQ=255)
                                           tgtATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCAc                                                    <  1:1700472/41‑1 (MQ=255)
                                             tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCa                                                     <  1:1127238/38‑1 (MQ=255)
                                             tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCa                                                     <  1:3323955/38‑1 (MQ=255)
                                             tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGc                                               <  1:2000538/44‑1 (MQ=255)
                                             tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCggtgg                              >  1:3860845/1‑61 (MQ=255)
                                             tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCggtgg                              >  1:4805118/1‑61 (MQ=255)
                                             tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCggtgg                              >  1:2581399/1‑61 (MQ=255)
                                             tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGgaaga                    <  1:4604790/71‑1 (MQ=255)
                                             tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGgaag                     >  1:4285458/1‑70 (MQ=255)
                                               tCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAAcgcg                                  >  1:1051697/1‑55 (MQ=255)
                                               tCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAAcgcg                                  >  1:1819863/1‑55 (MQ=255)
                                               tCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGgaagaa                   <  1:4547312/70‑1 (MQ=255)
                                                 cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGgaa                      >  1:848685/3‑65 (MQ=255)
                                                 cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGtc                >  1:4479915/3‑70 (MQ=255)
                                                 cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGtc                >  1:2887968/3‑70 (MQ=255)
                                                 cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGt                 >  1:1172157/3‑70 (MQ=255)
                                                 aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                 <  1:4557762/70‑1 (MQ=255)
                                                 aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg                <  1:4289954/71‑1 (MQ=255)
                                                 aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg                >  1:957472/1‑71 (MQ=255)
                                                 aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg                <  1:3622580/71‑1 (MQ=255)
                                                 aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg                >  1:374889/1‑71 (MQ=255)
                                                  ccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGtc                >  1:3668662/2‑69 (MQ=255)
                                                   cACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGTCGc              <  1:1034563/71‑1 (MQ=255)
                                                    aCGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                 >  1:3283436/1‑67 (MQ=255)
                                                    aCGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                 >  1:5679899/1‑67 (MQ=255)
                                                    aCGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGTCGct             <  1:744477/71‑2 (MQ=255)
                                                    aCGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGTCGct             >  1:648511/1‑70 (MQ=255)
                                                    aCGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAAcgccg                                 <  1:2246496/51‑3 (MQ=255)
                                                     cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCg             >  1:3421720/1‑70 (MQ=255)
                                                     cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGa            <  1:5533142/71‑1 (MQ=255)
                                                     cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGa            <  1:394521/71‑1 (MQ=255)
                                                      gggCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGa            >  1:80711/1‑70 (MQ=255)
                                                        gCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGAt                          >  1:4939775/1‑54 (MQ=255)
                                                        gCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaaa         >  1:209393/1‑71 (MQ=255)
                                                        gCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaaa         <  1:4340449/71‑1 (MQ=255)
                                                         ccGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaaa         <  1:4346794/70‑1 (MQ=255)
                                                           gTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCAt                                        <  1:4101342/37‑1 (MQ=255)
                                                           gTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCAt                                        <  1:3178707/37‑1 (MQ=255)
                                                           gTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATgg      >  1:1590732/1‑71 (MQ=255)
                                                            ttCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCggtggt                             <  1:3008695/47‑1 (MQ=255)
                                                              cGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCgg                                 >  1:3268981/1‑41 (MQ=255)
                                                              cGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCgg                                 >  1:3264103/1‑41 (MQ=255)
                                                              cGCCTGGGGGAAAGATGCTCAAGATGCGGTGCATAACGCGGTGGTGa                           >  1:3726122/1‑47 (MQ=255)
                                                               gCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg  <  1:4288885/71‑1 (MQ=255)
                                                               gCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg  <  1:4904463/71‑1 (MQ=255)
                                                               gCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg  <  1:4897729/71‑1 (MQ=255)
                                                                ccTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGgaaga                    <  1:3710590/52‑1 (MQ=255)
                                                                 |                                                                    
ACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTG  >  minE/2872189‑2872322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: