| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,872,305 | A→G | 7.6% | S186G (AGT→GGT) | ulaF → | L‑ribulose 5‑phosphate 4‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,872,305 | 0 | A | G | 7.6% | 259.7 / 8.6 | 92 | S186G (AGT→GGT) | ulaF | L‑ribulose 5‑phosphate 4‑epimerase |
| Reads supporting (aligned to +/- strand): ref base A (33/52); new base G (5/2); total (38/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
TCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGA > minE/2872236‑2872375 | tCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGgaagaa < 1:4547312/70‑1 (MQ=255) cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGtc > 1:2887968/3‑70 (MQ=255) cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGtc > 1:4479915/3‑70 (MQ=255) cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGt > 1:1172157/3‑70 (MQ=255) aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt < 1:4557762/70‑1 (MQ=255) aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg < 1:3622580/71‑1 (MQ=255) aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg < 1:4289954/71‑1 (MQ=255) ccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGtc > 1:3668662/2‑69 (MQ=255) cACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGTCGc < 1:1034563/71‑1 (MQ=255) aCGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt > 1:3283436/1‑67 (MQ=255) aCGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt > 1:5679899/1‑67 (MQ=255) aCGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGTCGct > 1:648511/1‑70 (MQ=255) aCGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGTCGct < 1:744477/71‑2 (MQ=255) cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCg > 1:3421720/1‑70 (MQ=255) cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGa < 1:394521/71‑1 (MQ=255) cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGa < 1:5533142/71‑1 (MQ=255) gggCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGa > 1:80711/1‑70 (MQ=255) gCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaaa < 1:4340449/71‑1 (MQ=255) gCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaaa > 1:209393/1‑71 (MQ=255) ccGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaaa < 1:4346794/70‑1 (MQ=255) gTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATgg > 1:1590732/1‑71 (MQ=255) gCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg < 1:4904463/71‑1 (MQ=255) gCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg < 1:4897729/71‑1 (MQ=255) gCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg < 1:4288885/71‑1 (MQ=255) tGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAt > 1:3644140/1‑71 (MQ=255) tGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAt > 1:5132382/1‑71 (MQ=255) tGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAt > 1:351066/1‑71 (MQ=255) gggggAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt < 1:1929678/52‑1 (MQ=255) ggggAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAt < 1:1433365/69‑1 (MQ=255) aaaGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt < 1:1849441/47‑1 (MQ=255) aaGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCcgc > 1:490196/1‑71 (MQ=255) aGATTCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt < 1:4170256/45‑1 (MQ=255) aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt < 1:429674/45‑1 (MQ=255) aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt < 1:2794660/45‑1 (MQ=255) aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt < 1:4422703/45‑1 (MQ=255) aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTgg > 1:3316616/1‑47 (MQ=255) aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTgg > 1:4602987/1‑47 (MQ=255) aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTgg > 1:148463/1‑47 (MQ=255) aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTgg > 1:2661147/1‑47 (MQ=255) aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaa < 1:1144192/51‑1 (MQ=255) aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaa < 1:4427771/51‑1 (MQ=255) aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaa < 1:4716890/51‑1 (MQ=255) aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaa < 1:1545982/51‑1 (MQ=255) aTGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCgg > 1:1123374/1‑70 (MQ=255) aTGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCgg > 1:4235227/1‑70 (MQ=255) gCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAt > 1:2695070/1‑71 (MQ=255) cTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt < 1:2629783/71‑1 (MQ=255) cTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAt < 1:796782/70‑1 (MQ=255) tCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt > 1:2634633/1‑70 (MQ=255) tCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAt < 1:66643/69‑1 (MQ=255) aCGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTaa < 1:1990982/70‑1 (MQ=255) aCGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAAc > 1:3027528/1‑71 (MQ=255) aCGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAAc > 1:803648/1‑71 (MQ=255) cGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg < 1:1080159/51‑1 (MQ=255) cGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg < 1:278121/51‑1 (MQ=255) cGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg < 1:1450193/51‑1 (MQ=255) aTGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCca > 1:5442204/1‑71 (MQ=255) aTGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCca > 1:3285736/1‑71 (MQ=255) tGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcac < 1:5400436/71‑1 (MQ=255) cGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaa < 1:3606104/37‑1 (MQ=255) cGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCcgc < 1:3874169/56‑1 (MQ=255) ggTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAAc < 1:4748852/71‑1 (MQ=255) gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt < 1:603351/60‑1 (MQ=255) gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt < 1:593340/60‑1 (MQ=255) gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt < 1:5330901/60‑1 (MQ=255) gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcac < 1:5342703/67‑1 (MQ=255) gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcac < 1:1541497/67‑1 (MQ=255) gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcac < 1:2913053/67‑1 (MQ=255) tGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAATTGGCGTg < 1:4648006/43‑1 (MQ=255) tGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg < 1:80033/43‑1 (MQ=255) tGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg < 1:4825754/43‑1 (MQ=255) tGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg < 1:3872119/43‑1 (MQ=255) tGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg < 1:1786805/43‑1 (MQ=255) cATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa < 1:4491733/70‑1 (MQ=255) aCGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGccc > 1:4066239/1‑45 (MQ=255) cgcgGTTGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATcaca < 1:1232952/71‑1 (MQ=255) cgcgGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt < 1:1778596/52‑1 (MQ=255) tggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcaca < 1:876268/55‑1 (MQ=255) tggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcaca < 1:1449565/55‑1 (MQ=255) tggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcaca < 1:2918272/55‑1 (MQ=255) tggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGa > 1:4586885/1‑70 (MQ=255) ggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGa > 1:2162000/1‑69 (MQ=255) gATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCcga > 1:1944767/1‑36 (MQ=255) tGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt < 1:4597065/41‑1 (MQ=255) tGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTc > 1:5028019/1‑71 (MQ=255) tGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTc > 1:5302567/1‑71 (MQ=255) tGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTc > 1:4346728/1‑71 (MQ=255) ggAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTcc > 1:1844077/1‑71 (MQ=255) ggAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTcc > 1:3516891/1‑71 (MQ=255) gaagaaGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCt > 1:5507983/1‑71 (MQ=255) gaagaaGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCt < 1:2532897/71‑1 (MQ=255) gaagaaGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCt > 1:3298989/1‑71 (MQ=255) gaagaaGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCt > 1:4298450/1‑71 (MQ=255) gaagaaGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCt < 1:1566369/71‑1 (MQ=255) gaagaaGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCt < 1:4811083/71‑1 (MQ=255) aagaagTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCtg < 1:3055171/71‑1 (MQ=255) aagaagTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCtg < 1:1014573/71‑1 (MQ=255) agaagTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa < 1:2554359/50‑1 (MQ=255) agTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCtgatga < 1:2529385/71‑1 (MQ=255) agTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCtgatg > 1:1131673/1‑70 (MQ=255) | TCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGA > minE/2872236‑2872375 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |