| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,911,777 | G→A | 14.0% | intergenic (+72/+134) | mgtA → / ← yjgF | magnesium transporter/ketoacid‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,911,777 | 0 | G | A | 14.0% | 156.9 / 13.9 | 64 | intergenic (+72/+134) | mgtA/yjgF | magnesium transporter/ketoacid‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (24/31); new base A (4/5); total (28/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
ATAAACTGGGCACGATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCA > minE/2911709‑2911846 | aTAAACTGGGCACGATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTa < 1:3290706/71‑1 (MQ=255) aaaCTGGGCACGATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTaaa > 1:100364/1‑71 (MQ=255) aaaCTGGGCACGATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTaaa > 1:4000063/1‑71 (MQ=255) aaCTGGGCACGATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTaa > 1:1414919/1‑69 (MQ=255) aaCTGGGCACGATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAAt > 1:2340116/1‑71 (MQ=255) aCTGGGCACGATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATg < 1:4279937/71‑1 (MQ=255) gggCACGATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTa < 1:1312315/64‑1 (MQ=255) ggCACGATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCtt < 1:2150770/71‑1 (MQ=255) gCACGATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGt < 1:550927/61‑1 (MQ=255) aCGATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTaa > 1:532283/1‑61 (MQ=255) aCGATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATc < 1:1393271/71‑1 (MQ=255) cGATAGCCCCGATTCAGATTGTAGACAAAGTGAGATTTGTTCATGCCGGATGCGGCGTa < 1:4294125/59‑1 (MQ=255) gATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGt < 1:4837651/57‑1 (MQ=255) gATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATcc < 1:5329835/70‑1 (MQ=255) gATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATcc > 1:5592202/1‑70 (MQ=255) gATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATc < 1:1027106/69‑1 (MQ=255) gATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCg > 1:2046015/1‑71 (MQ=255) aTAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCgg > 1:2608133/1‑71 (MQ=255) tAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGc > 1:5080350/1‑71 (MQ=255) aGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTaaa > 1:1647753/1‑57 (MQ=255) cttatcgtgccggaatacatcccatatttcggcacgctcaccggatgcGGCATCAGTGCATTATccggat > 1:5580942‑M2/49‑68 (MQ=255) cttatcgtgccggaatacatcccatatttcggcacgctcaccggatgcGGCATCAGTGCATTATccggat > 1:1035075‑M2/49‑68 (MQ=255) cttatcgtgccggaatacatcccatatttcggcacgctcaccggatgcGGCATCAGTGCATTATccggat > 1:2375641‑M2/49‑68 (MQ=255) ccGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCcaaca < 1:3010212/71‑1 (MQ=255) ccGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATACGGCGTAAATGCCTTATCCGGCcaac > 1:664712/1‑70 (MQ=255) tcgtgccggaatacatcccatatttcggcacgctcaccggatgcGGCATCAGTGCATTATCCGGAtaacaa < 1:1471091‑M2/27‑1 (MQ=255) tcgtgccggaatacatcccatatttcggcacgctcaccggatgcGGCATCAGTGCATTATCCGGAtaacaa < 1:548195‑M2/27‑1 (MQ=255) tcgtgccggaatacatcccatatttcggcacgctcaccggatgcGGCATCAGTGCATTATCCGGAtaacaa < 1:591027‑M2/27‑1 (MQ=255) gATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACaaa < 1:3773129/71‑1 (MQ=255) gtgccggaatacatcccatatttcggcacgctcaccggatgcGGCATCAGTGCATTATCCgg < 1:2777510‑M2/20‑1 (MQ=255) gtgccggaatacatcccatatttcggcacgctcaccggatgcGGCATCAGTGCATTATCCGgataacaata > 1:179359‑M2/43‑69 (MQ=255) gtgccggaatacatcccatatttcggcacgctcaccggatgcGGCATCAGTGCATTATCCGgataacaata < 1:1359697‑M2/29‑3 (MQ=255) aTTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTa < 1:2748961/48‑1 (MQ=255) aTTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTa < 1:3686778/48‑1 (MQ=255) ttCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTa < 1:2204925/47‑1 (MQ=255) tCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTa < 1:396003/46‑1 (MQ=255) tCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGc < 1:406676/51‑1 (MQ=255) aTTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAAt > 1:1179855/1‑70 (MQ=255) tAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTa < 1:816051/38‑1 (MQ=255) tAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTa < 1:2207174/38‑1 (MQ=255) gACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTaaa > 1:5045404/1‑38 (MQ=255) gACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTaaa > 1:4444367/1‑38 (MQ=255) gACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTg < 1:3545159/70‑1 (MQ=255) aCAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTa < 1:4234065/35‑1 (MQ=255) cAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAg > 1:1643894/1‑71 (MQ=255) gTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCgg > 1:1000726/1‑44 (MQ=255) gTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAAt < 1:4649130/71‑1 (MQ=255) gAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATcc > 1:1803372/1‑40 (MQ=255) aGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCCGCCAACAAATTCAATAAATTGCAGGAATCAc > 1:2271938/1‑71 (MQ=255) gTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGcc > 1:3416890/1‑42 (MQ=255) gTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCa > 1:2281783/1‑69 (MQ=255) ttGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAg > 1:812365/1‑71 (MQ=255) tCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCt > 1:3438460/1‑71 (MQ=255) cATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCt > 1:3526411/1‑70 (MQ=255) cATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCt < 1:4446270/70‑1 (MQ=255) aTGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGa < 1:2773408/71‑1 (MQ=255) aTGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGa < 1:5099439/71‑1 (MQ=255) ggatgcGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGc < 1:2635218‑M2/41‑1 (MQ=255) ggatgcGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGc < 1:3166951‑M2/41‑1 (MQ=255) tgcGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCg > 1:5076195‑M2/4‑69 (MQ=255) tgcGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTa < 1:4959696‑M2/68‑1 (MQ=255) tgcGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTa < 1:1182871‑M2/68‑1 (MQ=255) tgcGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTa < 1:1901803‑M2/68‑1 (MQ=255) cGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGc < 1:5184338‑M2/70‑1 (MQ=255) ggcgTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAAt < 1:4642394/47‑1 (MQ=255) ggcgTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAgcg < 1:4635045/71‑1 (MQ=255) ggcgTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAgcg < 1:3069909/71‑1 (MQ=255) ggcgTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAgcg < 1:4971433/71‑1 (MQ=255) ggcgTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAgcg < 1:4256496/71‑1 (MQ=255) gcgTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTa > 1:5058795/1‑52 (MQ=255) gcgTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTa > 1:1219835/1‑52 (MQ=255) gcgTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAgcgc < 1:2051417/71‑1 (MQ=255) gcgTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAgcgc < 1:3327339/71‑1 (MQ=255) cgTAAATGCCTTATCCTGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCa < 1:1377405/71‑1 (MQ=255) cgTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCa > 1:3306600/1‑71 (MQ=255) cgTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCa < 1:3934798/71‑1 (MQ=255) cgTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCa < 1:4497950/71‑1 (MQ=255) | ATAAACTGGGCACGATAGCCCCGATTCAGATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCA > minE/2911709‑2911846 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |