| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 312,705 | T→G | 20.0% | T56T (ACA→ACC) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 312,705 | 0 | T | G | 20.0% | 124.4 / 38.9 | 75 | T56T (ACA→ACC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (26/34); new base G (6/9); total (32/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
GGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGC > minE/312636‑312774 | ggACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTg < 1:4116180/71‑1 (MQ=255)ggACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTg < 1:277181/71‑1 (MQ=255) aCATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGtt < 1:3322075/71‑1 (MQ=255) cATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt < 1:3734500/71‑1 (MQ=255) cATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt < 1:2185003/71‑1 (MQ=255) aTGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGtttt > 1:1843486/1‑71 (MQ=255) tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCg < 1:4256768/70‑1 (MQ=255) tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCg < 1:4250009/70‑1 (MQ=255) tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCg < 1:3021689/70‑1 (MQ=255) tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGGCCTGCACTGtttt < 1:2766564/68‑1 (MQ=255) cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt < 1:1173121/71‑1 (MQ=255) cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt > 1:35407/1‑71 (MQ=255) cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt < 1:2673768/71‑1 (MQ=255) cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt < 1:1243728/71‑1 (MQ=255) tCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATc < 1:4368823/70‑1 (MQ=37) cAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGt > 1:1745536/1‑60 (MQ=37) cAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGt > 1:1705101/1‑60 (MQ=37) cAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGt > 1:3756695/1‑60 (MQ=37) aGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAg < 1:5294707/70‑1 (MQ=255) aGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAg < 1:3614084/70‑1 (MQ=255) aGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAg < 1:3307584/70‑1 (MQ=255) gTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAgcgc < 1:3808628/71‑1 (MQ=255) tATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCg > 1:979089/1‑71 (MQ=255) tATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCg > 1:5689768/1‑71 (MQ=255) tATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCg > 1:2702726/1‑71 (MQ=255) aTCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGtc > 1:1652766/1‑54 (MQ=37) cGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCa > 1:248889/1‑59 (MQ=25) agATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg > 1:3245035/2‑71 (MQ=255) gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg < 1:5188206/70‑1 (MQ=255) gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg < 1:4475126/70‑1 (MQ=255) gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg < 1:1161302/70‑1 (MQ=255) gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg < 1:269216/70‑1 (MQ=255) gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg < 1:125038/70‑1 (MQ=255) gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGt < 1:388676/71‑1 (MQ=255) gATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGTTCTGCGCATCAGCGCCcgga < 1:5384541/70‑2 (MQ=255) gATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCcggt < 1:1290593/70‑2 (MQ=255) aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt > 1:2680137/1‑52 (MQ=255) aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt > 1:4070017/1‑52 (MQ=255) aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt > 1:45095/1‑52 (MQ=255) aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg > 1:2838786/1‑69 (MQ=255) aCCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTag > 1:1469681/1‑70 (MQ=255) ccGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTc > 1:1595539/1‑51 (MQ=255) cGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATagttt < 1:3017300/71‑4 (MQ=255) gTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTaggagg > 1:3180705/1‑71 (MQ=255) ttCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGcc < 1:3628965/59‑1 (MQ=255) atatTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCgg < 1:2095546/70‑1 (MQ=255) tatTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATagt < 1:3632931/63‑2 (MQ=255) ttCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCa > 1:4439182/1‑48 (MQ=255) ttCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTagga > 1:3892778/1‑62 (MQ=255) ttCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATc < 1:3153770/47‑1 (MQ=255) tCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATagtt > 1:1346194/1‑59 (MQ=255) cTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATc < 1:180377/70‑1 (MQ=255) cTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCg > 1:2623153/1‑71 (MQ=255) cGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGgag < 1:2043722/62‑1 (MQ=255) cGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAg < 1:1227074/42‑1 (MQ=255) gATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTa > 1:356855/1‑68 (MQ=255) aTAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATag < 1:1605184/51‑1 (MQ=255) aTAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATag < 1:2063184/51‑1 (MQ=255) ccTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGt < 1:3274199/61‑1 (MQ=255) gtgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCcggcgg > 1:2464280/1‑70 (MQ=255) tgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCcggc < 1:758756/67‑1 (MQ=255) tgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGt < 1:3920667/70‑1 (MQ=255) tgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATAGTTACTGCCGGCGGt < 1:4952196/70‑1 (MQ=255) aCCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAAt > 1:1350858/1‑71 (MQ=255) aCCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAAt > 1:4572112/1‑71 (MQ=255) gtgtCCTGCACTGTTTTCGCATCAGCGCCGGTGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGt < 1:4952369/71‑1 (MQ=255) gtgtCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCg > 1:1962058/1‑70 (MQ=255) tgtCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTa > 1:2581657/1‑51 (MQ=255) cTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATc > 1:3605734/1‑43 (MQ=255) cTGCACTGTTTTCGCATCAGCGCCGGGGTAGCAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGa < 1:2107639/70‑1 (MQ=255) gCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGt < 1:4156464/43‑1 (MQ=255) gCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGatat > 1:2573190/1‑71 (MQ=255) cACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATAtt < 1:2573076/71‑1 (MQ=255) cACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATAtt > 1:1729493/1‑71 (MQ=255) cTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTgc > 1:969068/1‑71 (MQ=255) | GGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGC > minE/312636‑312774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |