BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	313,526	T→G	6.1%	N188T (AAC→ACC) ‡	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	313,526	0	T	G	6.1%	259.8 / 9.1	98	N188T (AAC→ACC) ‡	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand): ref base T (53/39); new base G (2/4); total (55/43)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 102 total aligned reads displayed.

CGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTC  >  minE/313456‑313596

cGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCTCCTGACCGTTCTGTACCAATGCGCCATCCGTCACGt                                                                        >  1:1003895/1‑71 (MQ=255)

 ggATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGt                                                                        <  1:4225191/70‑1 (MQ=255)

  gATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc                                                                      <  1:1810057/71‑1 (MQ=255)

  gATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc                                                                      <  1:1680452/71‑1 (MQ=255)

   aTCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc                                                                      <  1:5359403/70‑1 (MQ=255)

    tCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGa                                                                    <  1:1690203/71‑1 (MQ=255)

    tCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGa                                                                    >  1:3070621/1‑71 (MQ=255)

    tCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGa                                                                    >  1:4460138/1‑71 (MQ=255)

     cAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGa                                                                    >  1:5087788/1‑70 (MQ=255)

      aaGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGAc                                                                   >  1:4574650/1‑70 (MQ=255)

      aaGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGAc                                                                   >  1:2868680/1‑70 (MQ=255)

      aaGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACt                                                                  >  1:3475514/1‑71 (MQ=255)

      aaGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACt                                                                  >  1:1450698/1‑71 (MQ=255)

      aaGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACt                                                                  >  1:4640144/1‑71 (MQ=255)

      aaGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACt                                                                  >  1:1856073/1‑71 (MQ=255)

      aaGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACt                                                                  >  1:2749826/1‑71 (MQ=255)

      aaGTTGCTGCACGGGTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACt                                                                  <  1:1732072/71‑1 (MQ=255)

           gctgcACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTAcc                                                              <  1:540531/70‑1 (MQ=255)

           gctgcACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTAcc                                                              <  1:3693696/70‑1 (MQ=255)

             tgcACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCg                                                                     <  1:310165/61‑1 (MQ=255)

             tgcACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCg                                                                     <  1:3565365/61‑1 (MQ=255)

              gcACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAAt                                                           >  1:4312313/1‑70 (MQ=255)

               cACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                         >  1:5399426/1‑71 (MQ=255)

                aCGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                         >  1:1981536/1‑70 (MQ=255)

                aCGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCg                                                        <  1:1841616/71‑1 (MQ=255)

                 cGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc                                                                      <  1:5621440/56‑1 (MQ=255)

                  ggTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGAc                                                      >  1:1172146/1‑71 (MQ=255)

                   gTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                         <  1:1699255/67‑1 (MQ=255)

                   gTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                         <  1:3544861/67‑1 (MQ=255)

                     tGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGa                                                       >  1:2772511/1‑67 (MQ=255)

                     tGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGa                                                       >  1:2442808/1‑67 (MQ=255)

                     tGCCAGCGCAGTCGCCTGACCGCTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGa                                                       >  1:3024802/1‑67 (MQ=255)

                       ccAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTa                                                 <  1:5544201/71‑1 (MQ=255)

                         aGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAAt                                               >  1:3808787/1‑71 (MQ=255)

                          gcgcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATc                                              >  1:5354033/1‑71 (MQ=255)

                            gcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATc                                              >  1:3150097/1‑69 (MQ=255)

                            gcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCgg                                            <  1:2841279/71‑1 (MQ=255)

                             cAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                           <  1:2631577/71‑1 (MQ=255)

                             cAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGA‑CGCTAATCGga                                           <  1:4390026/70‑1 (MQ=255)

                               gTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                           >  1:1120198/1‑69 (MQ=255)

                                  gCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAgg                                       <  1:4347586/70‑1 (MQ=255)

                                       aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCt                                                  <  1:5126496/54‑1 (MQ=255)

                                       aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGa                                    >  1:1387643/1‑68 (MQ=255)

                                       aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACtt                                 >  1:1000774/1‑71 (MQ=255)

                                       aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACt                                  <  1:4078213/70‑1 (MQ=255)

                                        ccGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCgg                                            <  1:2773031/59‑1 (MQ=255)

                                        ccGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCgg                                            <  1:2058600/59‑1 (MQ=255)

                                         cGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                         >  1:2714395/1‑45 (MQ=255)

                                         cGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTaa                                                >  1:1363686/1‑54 (MQ=255)

                                          gTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGgag                                          >  1:2922155/1‑59 (MQ=255)

                                           ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACttt                                <  1:3908390/68‑1 (MQ=255)

                                           ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACttt                                <  1:1507394/68‑1 (MQ=255)

                                           ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                             >  1:4168834/1‑71 (MQ=255)

                                           ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                             >  1:1044453/1‑71 (MQ=255)

                                           ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                             >  1:1079468/1‑71 (MQ=255)

                                            tCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                            >  1:278007/1‑71 (MQ=255)

                                            tCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                            >  1:284897/1‑71 (MQ=255)

                                             cTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                            >  1:3060659/1‑70 (MQ=255)

                                                tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                           <  1:3958640/68‑1 (MQ=255)

                                                tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTa                          <  1:1234323/69‑1 (MQ=255)

                                                tACCAATGCGCCTTCCGTCACGTGCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                           <  1:2560667/68‑1 (MQ=255)

                                                 aCCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGc                       >  1:4053291/1‑71 (MQ=255)

                                                    aaTGCGGCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAg                    <  1:4957902/71‑1 (MQ=255)

                                                      tGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                            >  1:2424949/1‑61 (MQ=255)

                                                      tGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                            >  1:1280405/1‑61 (MQ=255)

                                                       gcgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                           >  1:877363/1‑45 (MQ=255)

                                                        cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                           >  1:609626/1‑44 (MQ=255)

                                                        cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                           >  1:885729/1‑44 (MQ=255)

                                                        cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                 >  1:1815974/1‑70 (MQ=255)

                                                        cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                 <  1:555686/70‑1 (MQ=255)

                                                        cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                 >  1:5604312/1‑70 (MQ=255)

                                                        cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                <  1:3291585/71‑1 (MQ=255)

                                                         gcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                <  1:345774/70‑1 (MQ=255)

                                                           cTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATc             >  1:5429045/1‑71 (MQ=255)

                                                            ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                       <  1:410310/60‑1 (MQ=255)

                                                            ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                       <  1:5609237/60‑1 (MQ=255)

                                                            ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                       <  1:4492323/60‑1 (MQ=255)

                                                            ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGAt              <  1:2698399/69‑1 (MQ=255)

                                                            ttCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAg                    <  1:333139/63‑1 (MQ=255)

                                                            ttCCGTCACGTTCGACTTACCAATACGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATc             >  1:1768711/1‑70 (MQ=255)

                                                             tcggTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGa               >  1:1674694/4‑67 (MQ=255)

                                                             tcggTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGa               >  1:2488414/4‑67 (MQ=255)

                                                              ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgc          >  1:747901/1‑71 (MQ=255)

                                                              ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgc          >  1:5309105/1‑71 (MQ=255)

                                                               cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg         <  1:2819622/71‑1 (MQ=255)

                                                               cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg         <  1:2231851/71‑1 (MQ=255)

                                                               cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg         >  1:3528495/1‑71 (MQ=255)

                                                                gTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                               <  1:4627500/48‑1 (MQ=255)

                                                                gTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                               <  1:1119593/48‑1 (MQ=255)

                                                                 tCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                           <  1:5474630/51‑1 (MQ=255)

                                                                 tCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa       <  1:2370381/71‑1 (MQ=255)

                                                                  cACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGa                   >  1:105230/1‑58 (MQ=255)

                                                                  cACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAg      >  1:1840239/1‑71 (MQ=255)

                                                                   aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                >  1:3201007/1‑60 (MQ=255)

                                                                   aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGAt              >  1:5252971/1‑62 (MQ=255)

                                                                   aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGt     >  1:3249714/1‑71 (MQ=255)

                                                                   aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACATTGGTGTAAGcc                      >  1:378175/1‑54 (MQ=255)

                                                                    cGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACtt                                 <  1:5043541/42‑1 (MQ=255)

                                                                      ttCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGc                       <  1:4100273/50‑1 (MQ=255)

                                                                      ttCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc  <  1:5258923/71‑1 (MQ=255)

CGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTC  >  minE/313456‑313596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`