Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	313,537	A→G	7.4%	I184I (ATT→ATC)	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	313,537	0	A	G	7.4%	268.0 / 8.8	94	I184I (ATT→ATC)	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base A (53/34);  new base G (2/5);  total (55/39)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTT  >  minE/313470‑313607                                                                    |                                                                       gcACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAAt                                                                      >  1:4312313/1‑70 (MQ=255)  cACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                                    >  1:5399426/1‑71 (MQ=255)   aCGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                                    >  1:1981536/1‑70 (MQ=255)   aCGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCg                                                                   <  1:1841616/71‑1 (MQ=255)     ggTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGAc                                                                 >  1:1172146/1‑71 (MQ=255)      gTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                                    <  1:1699255/67‑1 (MQ=255)      gTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                                    <  1:3544861/67‑1 (MQ=255)        tGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGa                                                                  >  1:2442808/1‑67 (MQ=255)        tGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGa                                                                  >  1:2772511/1‑67 (MQ=255)        tGCCAGCGCAGTCGCCTGACCGCTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGa                                                                  >  1:3024802/1‑67 (MQ=255)          ccAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCaa                                                                       >  1:3156769/1‑60 (MQ=255)          ccAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTa                                                            <  1:5544201/71‑1 (MQ=255)            aGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAAt                                                          >  1:3808787/1‑71 (MQ=255)             gcgcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATc                                                         >  1:5354033/1‑71 (MQ=255)               gcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATc                                                         >  1:3150097/1‑69 (MQ=255)               gcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCgg                                                       <  1:2841279/71‑1 (MQ=255)                cAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                      <  1:2631577/71‑1 (MQ=255)                cAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGA‑CGCTAATCGga                                                      <  1:4390026/70‑1 (MQ=255)                  gTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                      >  1:1120198/1‑69 (MQ=255)                     gCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAgg                                                  <  1:4347586/70‑1 (MQ=255)                          aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCt                                                             <  1:5126496/54‑1 (MQ=255)                          aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGa                                               >  1:1387643/1‑68 (MQ=255)                          aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACtt                                            >  1:1000774/1‑71 (MQ=255)                          aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACt                                             <  1:4078213/70‑1 (MQ=255)                           ccGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCgg                                                       <  1:2058600/59‑1 (MQ=255)                           ccGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCgg                                                       <  1:2773031/59‑1 (MQ=255)                            cGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                                    >  1:2714395/1‑45 (MQ=255)                            cGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTaa                                                           >  1:1363686/1‑54 (MQ=255)                             gTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGgag                                                     >  1:2922155/1‑59 (MQ=255)                              ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACttt                                           <  1:1507394/68‑1 (MQ=255)                              ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACttt                                           <  1:3908390/68‑1 (MQ=255)                              ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                        >  1:1044453/1‑71 (MQ=255)                              ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                        >  1:4168834/1‑71 (MQ=255)                              ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                        >  1:1079468/1‑71 (MQ=255)                               tCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                       >  1:278007/1‑71 (MQ=255)                               tCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                       >  1:284897/1‑71 (MQ=255)                                cTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                       >  1:3060659/1‑70 (MQ=255)                                   tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                      <  1:3958640/68‑1 (MQ=255)                                   tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTa                                     <  1:1234323/69‑1 (MQ=255)                                   tACCAATGCGCCTTCCGTCACGTGCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                      <  1:2560667/68‑1 (MQ=255)                                    aCCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGc                                  >  1:4053291/1‑71 (MQ=255)                                       aaTGCGGCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAg                               <  1:4957902/71‑1 (MQ=255)                                         tGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                       >  1:2424949/1‑61 (MQ=255)                                         tGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                       >  1:1280405/1‑61 (MQ=255)                                          gcgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                      >  1:877363/1‑45 (MQ=255)                                           cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                      >  1:609626/1‑44 (MQ=255)                                           cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                      >  1:885729/1‑44 (MQ=255)                                           cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                            >  1:5604312/1‑70 (MQ=255)                                           cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                            >  1:1815974/1‑70 (MQ=255)                                           cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                            <  1:555686/70‑1 (MQ=255)                                           cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                           <  1:3291585/71‑1 (MQ=255)                                            gcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                           <  1:345774/70‑1 (MQ=255)                                              cTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATc                        >  1:5429045/1‑71 (MQ=255)                                               ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                                  <  1:5609237/60‑1 (MQ=255)                                               ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                                  <  1:4492323/60‑1 (MQ=255)                                               ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                                  <  1:410310/60‑1 (MQ=255)                                               ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGAt                         <  1:2698399/69‑1 (MQ=255)                                               ttCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAg                               <  1:333139/63‑1 (MQ=255)                                               ttCCGTCACGTTCGACTTACCAATACGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATc                        >  1:1768711/1‑70 (MQ=255)                                                tcggTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGa                          >  1:2488414/4‑67 (MQ=255)                                                tcggTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGa                          >  1:1674694/4‑67 (MQ=255)                                                 ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgc                     >  1:747901/1‑71 (MQ=255)                                                 ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgc                     >  1:5309105/1‑71 (MQ=255)                                                 ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcg                      >  1:961809/1‑70 (MQ=255)                                                  cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                    >  1:3528495/1‑71 (MQ=255)                                                  cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                    <  1:2231851/71‑1 (MQ=255)                                                  cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                    <  1:2819622/71‑1 (MQ=255)                                                   gTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                          <  1:1119593/48‑1 (MQ=255)                                                   gTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                          <  1:4627500/48‑1 (MQ=255)                                                    tCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                      <  1:5474630/51‑1 (MQ=255)                                                    tCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                  <  1:2370381/71‑1 (MQ=255)                                                     cACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGa                              >  1:105230/1‑58 (MQ=255)                                                     cACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAg                 >  1:1840239/1‑71 (MQ=255)                                                      aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                           >  1:3201007/1‑60 (MQ=255)                                                      aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGAt                         >  1:5252971/1‑62 (MQ=255)                                                      aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGt                >  1:3249714/1‑71 (MQ=255)                                                      aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACATTGGTGTAAGcc                                 >  1:378175/1‑54 (MQ=255)                                                       cGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACtt                                            <  1:5043541/42‑1 (MQ=255)                                                         ttCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGc                                  <  1:4100273/50‑1 (MQ=255)                                                         ttCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc             <  1:5258923/71‑1 (MQ=255)                                                          tCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGAtt                            <  1:905778/55‑1 (MQ=255)                                                          tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                            >  1:3141002/1‑55 (MQ=255)                                                          tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                            >  1:3766842/1‑55 (MQ=255)                                                          tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                            >  1:3422879/1‑55 (MQ=255)                                                          tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                            >  1:2593473/1‑55 (MQ=255)                                                          tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGa                          >  1:3952600/1‑57 (MQ=255)                                                           cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                          >  1:5520484/1‑40 (MQ=255)                                                           cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                          >  1:28298/1‑40 (MQ=255)                                                           cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa           >  1:717827/1‑71 (MQ=255)                                                              cTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCa                                <  1:3053031/47‑1 (MQ=255)                                                              cTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCTCGCAGTTTCAAc          <  1:3527718/69‑1 (MQ=255)                                                                 aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa           >  1:1506094/1‑65 (MQ=255)                                                                 aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa           >  1:2968328/1‑65 (MQ=255)                                                                 aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAg     >  1:1059598/1‑71 (MQ=255)                                                                   cAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCt   <  1:3454192/71‑1 (MQ=255)                                                                    aaTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCtt  <  1:5120344/71‑1 (MQ=255)                                                                    |                                                                       GCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTT  >  minE/313470‑313607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑