BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	313,540	G→A	7.9%	R183R (CGC→CGT)	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	313,540	0	G	A	7.9%	291.3 / 10.2	101	R183R (CGC→CGT)	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand): ref base G (53/40); new base A (3/5); total (56/45)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

Only 100 of 104 total aligned reads displayed.

CACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCG  >  minE/313471‑313610

cACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                                       >  1:5399426/1‑71 (MQ=255)

 aCGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                                       >  1:1981536/1‑70 (MQ=255)

 aCGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCg                                                                      <  1:1841616/71‑1 (MQ=255)

   ggTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGAc                                                                    >  1:1172146/1‑71 (MQ=255)

    gTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                                       <  1:3544861/67‑1 (MQ=255)

    gTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                                       <  1:1699255/67‑1 (MQ=255)

      tGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGa                                                                     >  1:2772511/1‑67 (MQ=255)

      tGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGa                                                                     >  1:2442808/1‑67 (MQ=255)

      tGCCAGCGCAGTCGCCTGACCGCTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGa                                                                     >  1:3024802/1‑67 (MQ=255)

        ccAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTa                                                               <  1:5544201/71‑1 (MQ=255)

          aGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAAt                                                             >  1:3808787/1‑71 (MQ=255)

           gcgcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATc                                                            >  1:5354033/1‑71 (MQ=255)

             gcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATc                                                            >  1:3150097/1‑69 (MQ=255)

             gcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCgg                                                          <  1:2841279/71‑1 (MQ=255)

              cAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                         <  1:2631577/71‑1 (MQ=255)

              cAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGA‑CGCTAATCGga                                                         <  1:4390026/70‑1 (MQ=255)

                gTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                         >  1:1120198/1‑69 (MQ=255)

                   gCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAgg                                                     <  1:4347586/70‑1 (MQ=255)

                        aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCt                                                                <  1:5126496/54‑1 (MQ=255)

                        aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGa                                                  >  1:1387643/1‑68 (MQ=255)

                        aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACtt                                               >  1:1000774/1‑71 (MQ=255)

                        aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACt                                                <  1:4078213/70‑1 (MQ=255)

                         ccGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCgg                                                          <  1:2058600/59‑1 (MQ=255)

                         ccGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCgg                                                          <  1:2773031/59‑1 (MQ=255)

                          cGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                                       >  1:2714395/1‑45 (MQ=255)

                          cGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTaa                                                              >  1:1363686/1‑54 (MQ=255)

                           gTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGgag                                                        >  1:2922155/1‑59 (MQ=255)

                            ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACttt                                              <  1:1507394/68‑1 (MQ=255)

                            ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACttt                                              <  1:3908390/68‑1 (MQ=255)

                            ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                           >  1:4168834/1‑71 (MQ=255)

                            ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                           >  1:1044453/1‑71 (MQ=255)

                            ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                           >  1:1079468/1‑71 (MQ=255)

                             tCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                          >  1:278007/1‑71 (MQ=255)

                             tCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                          >  1:284897/1‑71 (MQ=255)

                              cTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                          >  1:3060659/1‑70 (MQ=255)

                                 tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                         <  1:3958640/68‑1 (MQ=255)

                                 tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTa                                        <  1:1234323/69‑1 (MQ=255)

                                 tACCAATGCGCCTTCCGTCACGTGCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                         <  1:2560667/68‑1 (MQ=255)

                                  aCCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGc                                     >  1:4053291/1‑71 (MQ=255)

                                     aaTGCGGCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAg                                  <  1:4957902/71‑1 (MQ=255)

                                       tGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                          >  1:1280405/1‑61 (MQ=255)

                                       tGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                          >  1:2424949/1‑61 (MQ=255)

                                        gcgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                         >  1:877363/1‑45 (MQ=255)

                                         cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                         >  1:885729/1‑44 (MQ=255)

                                         cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                         >  1:609626/1‑44 (MQ=255)

                                         cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                               <  1:555686/70‑1 (MQ=255)

                                         cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                               >  1:1815974/1‑70 (MQ=255)

                                         cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                               >  1:5604312/1‑70 (MQ=255)

                                          gcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                              <  1:345774/70‑1 (MQ=255)

                                            cTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATc                           >  1:5429045/1‑71 (MQ=255)

                                             ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                                     <  1:410310/60‑1 (MQ=255)

                                             ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                                     <  1:5609237/60‑1 (MQ=255)

                                             ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                                     <  1:4492323/60‑1 (MQ=255)

                                             ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGAt                            <  1:2698399/69‑1 (MQ=255)

                                             ttCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAg                                  <  1:333139/63‑1 (MQ=255)

                                             ttCCGTCACGTTCGACTTACCAATACGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATc                           >  1:1768711/1‑70 (MQ=255)

                                              tcggTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGa                             >  1:2488414/4‑67 (MQ=255)

                                              tcggTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGa                             >  1:1674694/4‑67 (MQ=255)

                                               ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgc                        >  1:747901/1‑71 (MQ=255)

                                               ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgc                        >  1:5309105/1‑71 (MQ=255)

                                                cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                       >  1:3528495/1‑71 (MQ=255)

                                                cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                       <  1:2819622/71‑1 (MQ=255)

                                                cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                       <  1:2231851/71‑1 (MQ=255)

                                                 gTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                             <  1:1119593/48‑1 (MQ=255)

                                                  tCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                         <  1:5474630/51‑1 (MQ=255)

                                                  tCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                     <  1:2370381/71‑1 (MQ=255)

                                                   cACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGa                                 >  1:105230/1‑58 (MQ=255)

                                                   cACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAg                    >  1:1840239/1‑71 (MQ=255)

                                                    aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                              >  1:3201007/1‑60 (MQ=255)

                                                    aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGAt                            >  1:5252971/1‑62 (MQ=255)

                                                    aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGt                   >  1:3249714/1‑71 (MQ=255)

                                                    aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACATTGGTGTAAGcc                                    >  1:378175/1‑54 (MQ=255)

                                                     cGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACtt                                               <  1:5043541/42‑1 (MQ=255)

                                                       ttCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGc                                     <  1:4100273/50‑1 (MQ=255)

                                                       ttCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                <  1:5258923/71‑1 (MQ=255)

                                                        tCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGAtt                               <  1:905778/55‑1 (MQ=255)

                                                        tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                               >  1:3766842/1‑55 (MQ=255)

                                                        tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                               >  1:2593473/1‑55 (MQ=255)

                                                        tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                               >  1:3422879/1‑55 (MQ=255)

                                                        tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                               >  1:3141002/1‑55 (MQ=255)

                                                        tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGa                             >  1:3952600/1‑57 (MQ=255)

                                                         cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                             >  1:28298/1‑40 (MQ=255)

                                                         cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                             >  1:5520484/1‑40 (MQ=255)

                                                         cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa              >  1:717827/1‑71 (MQ=255)

                                                            cTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCa                                   <  1:3053031/47‑1 (MQ=255)

                                                            cTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCTCGCAGTTTCAAc             <  1:3527718/69‑1 (MQ=255)

                                                               aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa              >  1:2968328/1‑65 (MQ=255)

                                                               aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa              >  1:1506094/1‑65 (MQ=255)

                                                               aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAg        >  1:1059598/1‑71 (MQ=255)

                                                                 cAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCt      <  1:3454192/71‑1 (MQ=255)

                                                                  aaTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCtt     <  1:5120344/71‑1 (MQ=255)

                                                                   aTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                <  1:1477636/59‑1 (MQ=255)

                                                                   aTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                <  1:5623541/59‑1 (MQ=255)

                                                                   aTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                <  1:4571945/59‑1 (MQ=255)

                                                                    tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                       >  1:1829267/1‑51 (MQ=255)

                                                                    tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa              >  1:3440844/1‑60 (MQ=255)

                                                                    tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTc   <  1:4584586/71‑1 (MQ=255)

                                                                    tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGAACcgcga                      <  1:3967292/52‑2 (MQ=255)

                                                                     gCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa              >  1:712816/1‑59 (MQ=255)

                                                                     gCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcg  >  1:5637393/1‑71 (MQ=255)

CACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCG  >  minE/313471‑313610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`