BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	313,543	A→T	7.0%	G182G (GGT→GGA)	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	313,543	0	A	T	7.0%	263.0 / 8.7	98	G182G (GGT→GGA)	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand): ref base A (54/37); new base T (2/5); total (56/42)
Fisher's exact test for biased strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 105 total aligned reads displayed.

GGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCG  >  minE/313474‑313613

ggTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGAc                                                                       >  1:1172146/1‑71 (MQ=255)

   tGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGa                                                                        >  1:2442808/1‑67 (MQ=255)

   tGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGa                                                                        >  1:2772511/1‑67 (MQ=255)

     ccAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTa                                                                  <  1:5544201/71‑1 (MQ=255)

       aGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAAt                                                                >  1:3808787/1‑71 (MQ=255)

        gcgcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATc                                                               >  1:5354033/1‑71 (MQ=255)

          gcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATc                                                               >  1:3150097/1‑69 (MQ=255)

          gcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCgg                                                             <  1:2841279/71‑1 (MQ=255)

           cAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                            <  1:2631577/71‑1 (MQ=255)

           cAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGA‑CGCTAATCGga                                                            <  1:4390026/70‑1 (MQ=255)

             gTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                            >  1:1120198/1‑69 (MQ=255)

                gCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAgg                                                        <  1:4347586/70‑1 (MQ=255)

                     aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCt                                                                   <  1:5126496/54‑1 (MQ=255)

                     aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGa                                                     >  1:1387643/1‑68 (MQ=255)

                     aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACtt                                                  >  1:1000774/1‑71 (MQ=255)

                     aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACt                                                   <  1:4078213/70‑1 (MQ=255)

                      ccGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCgg                                                             <  1:2773031/59‑1 (MQ=255)

                       cGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTaa                                                                 >  1:1363686/1‑54 (MQ=255)

                        gTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGgag                                                           >  1:2922155/1‑59 (MQ=255)

                         ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACttt                                                 <  1:1507394/68‑1 (MQ=255)

                         ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                              >  1:1044453/1‑71 (MQ=255)

                         ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                              >  1:4168834/1‑71 (MQ=255)

                         ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                              >  1:1079468/1‑71 (MQ=255)

                          tCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                             >  1:284897/1‑71 (MQ=255)

                          tCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                             >  1:278007/1‑71 (MQ=255)

                           cTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                             >  1:3060659/1‑70 (MQ=255)

                              tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                            <  1:3958640/68‑1 (MQ=255)

                              tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTa                                           <  1:1234323/69‑1 (MQ=255)

                              tACCAATGCGCCTTCCGTCACGTGCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                            <  1:2560667/68‑1 (MQ=255)

                               aCCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGc                                        >  1:4053291/1‑71 (MQ=255)

                                  aaTGCGGCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAg                                     <  1:4957902/71‑1 (MQ=255)

                                    tGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                             >  1:2424949/1‑61 (MQ=255)

                                    tGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                             >  1:1280405/1‑61 (MQ=255)

                                     gcgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                            >  1:877363/1‑45 (MQ=255)

                                      cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                            >  1:609626/1‑44 (MQ=255)

                                      cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                            >  1:885729/1‑44 (MQ=255)

                                      cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                  <  1:555686/70‑1 (MQ=255)

                                      cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                  >  1:1815974/1‑70 (MQ=255)

                                      cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                  >  1:5604312/1‑70 (MQ=255)

                                      cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                                 <  1:3291585/71‑1 (MQ=255)

                                       gcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                                 <  1:345774/70‑1 (MQ=255)

                                         cTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATc                              >  1:5429045/1‑71 (MQ=255)

                                          ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                                        <  1:5609237/60‑1 (MQ=255)

                                          ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                                        <  1:4492323/60‑1 (MQ=255)

                                          ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                                        <  1:410310/60‑1 (MQ=255)

                                          ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGAt                               <  1:2698399/69‑1 (MQ=255)

                                          ttCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAg                                     <  1:333139/63‑1 (MQ=255)

                                          ttCCGTCACGTTCGACTTACCAATACGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATc                              >  1:1768711/1‑70 (MQ=255)

                                           tcggTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGa                                >  1:2488414/4‑67 (MQ=255)

                                           tcggTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGa                                >  1:1674694/4‑67 (MQ=255)

                                            ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgc                           >  1:747901/1‑71 (MQ=255)

                                            ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgc                           >  1:5309105/1‑71 (MQ=255)

                                             cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                          <  1:2819622/71‑1 (MQ=255)

                                             cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                          >  1:3528495/1‑71 (MQ=255)

                                             cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                          <  1:2231851/71‑1 (MQ=255)

                                              gTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                                <  1:4627500/48‑1 (MQ=255)

                                              gTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                                <  1:1119593/48‑1 (MQ=255)

                                               tCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                            <  1:5474630/51‑1 (MQ=255)

                                               tCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                        <  1:2370381/71‑1 (MQ=255)

                                                cACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGa                                    >  1:105230/1‑58 (MQ=255)

                                                cACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAg                       >  1:1840239/1‑71 (MQ=255)

                                                 aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                                 >  1:3201007/1‑60 (MQ=255)

                                                 aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGt                      >  1:3249714/1‑71 (MQ=255)

                                                 aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACATTGGTGTAAGcc                                       >  1:378175/1‑54 (MQ=255)

                                                  cGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACtt                                                  <  1:5043541/42‑1 (MQ=255)

                                                    ttCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGc                                        <  1:4100273/50‑1 (MQ=255)

                                                    ttCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                   <  1:5258923/71‑1 (MQ=255)

                                                     tCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGAtt                                  <  1:905778/55‑1 (MQ=255)

                                                     tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                  >  1:2593473/1‑55 (MQ=255)

                                                     tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                  >  1:3766842/1‑55 (MQ=255)

                                                     tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                  >  1:3141002/1‑55 (MQ=255)

                                                     tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                  >  1:3422879/1‑55 (MQ=255)

                                                     tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGa                                >  1:3952600/1‑57 (MQ=255)

                                                      cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                                >  1:28298/1‑40 (MQ=255)

                                                      cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                                >  1:5520484/1‑40 (MQ=255)

                                                      cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                 >  1:717827/1‑71 (MQ=255)

                                                         cTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCa                                      <  1:3053031/47‑1 (MQ=255)

                                                         cTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCTCGCAGTTTCAAc                <  1:3527718/69‑1 (MQ=255)

                                                            aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                 >  1:1506094/1‑65 (MQ=255)

                                                            aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                 >  1:2968328/1‑65 (MQ=255)

                                                            aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAg           >  1:1059598/1‑71 (MQ=255)

                                                              cAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCt         <  1:3454192/71‑1 (MQ=255)

                                                               aaTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCtt        <  1:5120344/71‑1 (MQ=255)

                                                                aTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                   <  1:1477636/59‑1 (MQ=255)

                                                                aTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                   <  1:4571945/59‑1 (MQ=255)

                                                                aTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                   <  1:5623541/59‑1 (MQ=255)

                                                                 tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                          >  1:1829267/1‑51 (MQ=255)

                                                                 tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                 >  1:3440844/1‑60 (MQ=255)

                                                                 tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTc      <  1:4584586/71‑1 (MQ=255)

                                                                 tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGAACcgcga                         <  1:3967292/52‑2 (MQ=255)

                                                                  gCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                 >  1:712816/1‑59 (MQ=255)

                                                                  gCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                 >  1:2261863/1‑59 (MQ=255)

                                                                  gCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcg     >  1:5637393/1‑71 (MQ=255)

                                                                   cGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGa                                    >  1:3357281/1‑39 (MQ=255)

                                                                   cGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGa                                    >  1:5145249/1‑39 (MQ=255)

                                                                   cGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCa            >  1:3694373/1‑63 (MQ=255)

                                                                     aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                        <  1:2506763/49‑1 (MQ=255)

                                                                     aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                        <  1:823128/49‑1 (MQ=255)

                                                                     aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                        <  1:4850801/49‑1 (MQ=255)

                                                                     aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccg  <  1:3844898/71‑1 (MQ=255)

GGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCG  >  minE/313474‑313613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`