| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,919,786 | C→T | 8.5% | intergenic (‑91/‑71) | argI ← / → yjgD | ornithine carbamoyltransferase 1/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,919,786 | 0 | C | T | 8.5% | 441.8 / 19.2 | 153 | intergenic (‑91/‑71) | argI/yjgD | ornithine carbamoyltransferase 1/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (84/56); new base T (7/6); total (91/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 160 total aligned reads displayed.
GCCTCAATGAATTAAAATTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGC > minE/2919716‑2919855 | aaTGAATTAAAATTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTcaca > 1:3737516/1‑69 (MQ=255) aaTGAATTAAAATTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACaaa < 1:725143/71‑1 (MQ=255) tGAATTAAAATTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTcaca < 1:4754993/67‑1 (MQ=255) gAATTAAAATTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGt > 1:2808177/1‑71 (MQ=255) aaTTAAAATTCACTTTATATGTGTAATTATTCATTTGCAACCCCATTTCACAATTCTTTCTTACAAAGGTg < 1:2476002/71‑1 (MQ=255) aTTAAAATTCACTTTATATGTGTAATTATTCATTTGCAACCCCATTTCACAATTCTTTCTTACAAAGGTg < 1:552584/70‑1 (MQ=255) ttAAAATTCAATTTTTATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTgg < 1:3479434/70‑1 (MQ=255) aaaaTTCACTTTATATGTGTAATTATTCATTTGCACCCCCATTTCACAATTCTTTCTTACAAAGGTGGAgg > 1:4112108/1‑71 (MQ=255) aaaTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAgg < 1:4147958/70‑1 (MQ=255) aaaTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGc < 1:4741111/71‑1 (MQ=255) aaTTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGc < 1:1688589/70‑1 (MQ=255) tttCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACaaa < 1:5648880/59‑1 (MQ=255) aTTCACTTTATATGTGTAATTATTCATTTGCAACCCCATTTCACAATTCTTTCTTACAAAGGTGGAGGCaa < 1:2416299/71‑1 (MQ=255) ttCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCaa > 1:346257/1‑70 (MQ=255) ttCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCaa > 1:3072625/1‑70 (MQ=255) ttCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAAt > 1:5313446/1‑71 (MQ=255) tCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAgg > 1:4259186/1‑60 (MQ=255) tCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCaa > 1:5297771/1‑69 (MQ=255) cAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAgg > 1:5261381/1‑59 (MQ=255) aTTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCa < 1:496189/71‑1 (MQ=255) aTTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCa < 1:667106/71‑1 (MQ=255) tataTGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAgtg > 1:1443233/1‑71 (MQ=255) atatGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAgtgg > 1:3991974/1‑71 (MQ=255) tGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTc > 1:5444318/1‑43 (MQ=255) tGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAgtggtg < 1:1991408/70‑1 (MQ=255) gatgatTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAgg > 1:2127078/1‑48 (MQ=255) gatgatTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAgg > 1:1215417/1‑48 (MQ=255) gatgatTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGgtgtgt > 1:3009926/1‑71 (MQ=255) atgatTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAg > 1:1835171/1‑52 (MQ=255) gatTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAgg > 1:3771574/1‑45 (MQ=255) gatTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAgg > 1:1964015/1‑45 (MQ=255) attattCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGAc > 1:2177611/1‑70 (MQ=255) ttattCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCaa > 1:1234272/1‑52 (MQ=255) ttattCATTTGCAACCCCATTTCACAATTCTTTCTTACAAAGGTg < 1:3937787/45‑1 (MQ=255) tattCATTTGCAAGTCTAAAGCATAAATCTTTGTCACaa < 1:3648732/39‑1 (MQ=255) ttCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAAt > 1:2149397/1‑69 (MQ=255) ttCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATaa < 1:3577876/71‑1 (MQ=255) tCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAAt > 1:1000203/1‑68 (MQ=255) aTTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAagag > 1:851574/1‑71 (MQ=255) aTTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAagag > 1:3890303/1‑71 (MQ=255) tttGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCa < 1:1173437/50‑1 (MQ=255) tGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAgt > 1:4966426/1‑50 (MQ=255) tGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAgt > 1:2665336/1‑50 (MQ=255) tGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTa < 1:518456/70‑1 (MQ=255) tGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTAt > 1:5344715/1‑71 (MQ=255) tGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAAAAAGAGTAt > 1:4699726/1‑71 (MQ=255) gCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTg < 1:2487863/35‑1 (MQ=255) cAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGa < 1:2657415/57‑1 (MQ=255) aaGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGgtgtgt < 1:3225505/54‑1 (MQ=255) aaGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCgg < 1:2293791/71‑1 (MQ=255) aGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGc < 1:5530268/71‑1 (MQ=255) gTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGc < 1:1558451/36‑1 (MQ=255) gTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGc < 1:4891607/36‑1 (MQ=255) gTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAg < 1:3053669/44‑1 (MQ=255) gTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGAc < 1:1005353/55‑1 (MQ=255) tCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAg < 1:4546108/71‑1 (MQ=255) cTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAg > 1:2026998/1‑70 (MQ=255) tAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGa > 1:3682534/1‑71 (MQ=255) tAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGa > 1:3378416/1‑71 (MQ=255) tAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGa > 1:2877922/1‑71 (MQ=255) tAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGa > 1:299969/1‑71 (MQ=255) tAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGa > 1:1570164/1‑71 (MQ=255) tAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGa > 1:3528147/1‑71 (MQ=255) tAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGa > 1:5600443/1‑71 (MQ=255) tAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGa > 1:4540978/1‑71 (MQ=255) aaGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGa < 1:4001974/69‑1 (MQ=255) aaGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGa < 1:3125529/69‑1 (MQ=255) aaGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGAc < 1:4459273/70‑1 (MQ=255) aaGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACa > 1:5501872/1‑71 (MQ=255) aGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACAt < 1:4154781/71‑1 (MQ=255) tAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAAt < 1:1136269/48‑1 (MQ=255) tAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAagag > 1:4209560/1‑53 (MQ=255) tAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAagag > 1:4202811/1‑53 (MQ=255) aaaTCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGa < 1:4790631/43‑1 (MQ=255) aaaTCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGa < 1:4883095/43‑1 (MQ=255) aaaTCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGAAGGACATTaa < 1:3168621/69‑1 (MQ=255) aaTCTTTGTCACAAAGGTGGAGGCAATGTCAgtggtg > 1:4848204/1‑37 (MQ=255) aaTCTTTGTCACAAAGGTGGAGGCAATGTCAgtggtg > 1:2773370/1‑37 (MQ=255) aaTCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTaa > 1:615444/1‑68 (MQ=255) aaTCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAagag < 1:1123610/71‑1 (MQ=255) aaTCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAagag < 1:4443757/71‑1 (MQ=255) aTCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGAc > 1:5053565/1‑42 (MQ=255) aTCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAagag > 1:4933423/1‑70 (MQ=255) aTCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAagag > 1:1193032/1‑70 (MQ=255) aTCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAgg < 1:1490840/71‑1 (MQ=255) aTCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAgg < 1:1880655/71‑1 (MQ=255) aTCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAgg < 1:1066473/71‑1 (MQ=255) cTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGa > 1:1345603/1‑39 (MQ=255) cTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGa > 1:5594556/1‑39 (MQ=255) cTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGa > 1:3165966/1‑39 (MQ=255) cTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACa > 1:351258/1‑41 (MQ=255) cTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGaa > 1:1812894/1‑71 (MQ=255) tttGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAagag < 1:1536260/47‑1 (MQ=255) tttGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAagag < 1:2573747/47‑1 (MQ=255) tGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAagag > 1:730605/1‑45 (MQ=255) tGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTa > 1:3804617/1‑47 (MQ=255) tGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTa > 1:4772066/1‑47 (MQ=255) tGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTaa < 1:5169190/62‑1 (MQ=255) tGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAAtt < 1:3418197/70‑2 (MQ=255) tCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGc < 1:5083344/71‑1 (MQ=255) | GCCTCAATGAATTAAAATTCAATTTATATGGATGATTATTCATTTGCAAGTCTAAAGCATAAATCTTTGTCACAAAGGTGGAGGCAATGTCAGTGGTGTGTGACAATAAGAGTATCGGCAGGACATTAAGAGGAATGAGC > minE/2919716‑2919855 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |