| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,940,923 | C→A | 11.8% | G180C (GGC→TGC) | fecC ← | iron‑dicitrate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,940,923 | 0 | C | A | 11.8% | 308.9 / 16.6 | 101 | G180C (GGC→TGC) | fecC | iron‑dicitrate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (44/45); new base A (4/8); total (48/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
AAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGAT > minE/2940859‑2940992 | aaGAGCTGCCAGACATCCTGCCAGCGGGTGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGt < 1:1680369/67‑1 (MQ=255) agCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCa > 1:3928100/1‑69 (MQ=255) agCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCAt > 1:2804368/1‑70 (MQ=255) agCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATg < 1:3311750/71‑1 (MQ=255) gCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGa > 1:5677543/1‑71 (MQ=255) tGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTa < 1:866669/62‑1 (MQ=255) ccAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCtt > 1:4696969/1‑71 (MQ=255) ccAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCtt < 1:5536495/71‑1 (MQ=255) ccAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCt < 1:851021/70‑1 (MQ=255) aGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCg > 1:2440963/1‑71 (MQ=255) cATCCTGCCAGCGGGCGTTGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGcc < 1:728398/71‑1 (MQ=255) aTCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATc > 1:4623525/1‑63 (MQ=255) gCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCcagcag > 1:4538324/1‑70 (MQ=255) ccAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCcagcagca < 1:899833/71‑1 (MQ=255) cAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCcagcagcag < 1:4445747/71‑1 (MQ=255) cAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCcagcagcag > 1:2600287/1‑71 (MQ=255) cAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCcagcagcag > 1:15255/1‑71 (MQ=255) gCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCgg < 1:1869783/59‑1 (MQ=255) gCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAggg < 1:4979176/71‑1 (MQ=255) cGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGt < 1:2335046/71‑1 (MQ=255) gggCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGt > 1:1040648/1‑70 (MQ=255) taccggttggtttagcgagaagAGCCAGTAAAAGACGCAGt < 1:4281735‑M2/19‑1 (MQ=255) taccggttgggttagcgagaagAGCCAGTAAAAGACGCAGt < 1:315000‑M2/19‑1 (MQ=255) accggttgggttagcgagaagAGCCAGTAAAAGACGCAGt > 1:221378‑M2/22‑40 (MQ=255) accggttgggttagcgagaagAGCCAGTAAAAGACGCAGt > 1:4638053‑M2/22‑40 (MQ=255) accggttgggttagcgagaagAGCCAGTAAAAGACGCAGt > 1:311424‑M2/22‑40 (MQ=255) accggttgggttagcgagaagAGCCAGTAAAAGACGCAGt > 1:5203469‑M2/22‑40 (MQ=255) cGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATg < 1:1323210/71‑1 (MQ=255) gTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGc > 1:1657209/1‑71 (MQ=255) gTGGCACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGc < 1:4619452/71‑1 (MQ=255) cggttgggttagcgagaagAGCCAGTAAAAGACGCAGt < 1:3476691‑M2/19‑1 (MQ=255) cggttgggttagcgagaagAGCCAGTAAAAGACGCAGt < 1:296584‑M2/19‑1 (MQ=255) gttgggttagcgagaagAGCCAGTAAAAGACGCAGt < 1:5605238‑M2/19‑1 (MQ=255) gttgggttagcgagaagAGCCAGTAAAAGACGCAGt < 1:4304800‑M2/19‑1 (MQ=255) gggACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCtt > 1:3187980/1‑48 (MQ=255) gggACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCgg > 1:1920005/1‑71 (MQ=255) ttgggttagcgagaagAGCCAGTAAAAGACGCAGt < 1:5464082‑M2/19‑1 (MQ=255) ttgggttagcgagaagAGCCAGTAAAAGACGCAGt < 1:3815422‑M2/19‑1 (MQ=255) ggACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGa > 1:396076/1‑43 (MQ=255) gACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGt < 1:5315348/71‑1 (MQ=255) gACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGt < 1:5000159/71‑1 (MQ=255) acacTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCcagcagc > 1:2260745/1‑56 (MQ=255) acacTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCcagcagc > 1:2478118/1‑56 (MQ=255) acacTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGt > 1:2037066/1‑70 (MQ=255) acacTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTa < 1:1299109/71‑1 (MQ=255) acacTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTa < 1:3417407/71‑1 (MQ=255) acTCCGCCTGCGAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGt < 1:2841071/68‑1 (MQ=255) acTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAg > 1:1212340/1‑71 (MQ=255) cTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGt < 1:844676/67‑1 (MQ=255) ccGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTc > 1:2409728/1‑41 (MQ=255) ccGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGcc > 1:4873813/1‑71 (MQ=255) ccGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGcc > 1:806127/1‑71 (MQ=255) cGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGAt > 1:2516497/1‑36 (MQ=255) cGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGAt > 1:155178/1‑36 (MQ=255) cGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGcc > 1:4559467/1‑70 (MQ=255) cGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGcc > 1:4806669/1‑70 (MQ=255) cGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGcc > 1:3849812/1‑70 (MQ=255) cGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGcc < 1:3160664/70‑1 (MQ=255) tGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATa > 1:1464831/1‑70 (MQ=255) tGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATa > 1:3405125/1‑70 (MQ=255) gccagccaGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATAc > 1:979894/1‑70 (MQ=255) ccagccagTAAAAGATGCCGTAAGCATGATCTTCGGCcagcag < 1:965132/43‑1 (MQ=255) ccagccagTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAggg < 1:3761631/48‑1 (MQ=255) ccagccagTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAggg < 1:4773106/48‑1 (MQ=255) ccagccagTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAggg < 1:3583650/48‑1 (MQ=255) ccagccagTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACaa < 1:2575713/71‑1 (MQ=255) ccagccagTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACaa < 1:63706/71‑1 (MQ=255) ccagccagTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACa < 1:3190775/70‑1 (MQ=255) agccagTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGa > 1:4863534/1‑49 (MQ=255) ccagTAAAAGATGCCGTAAGCATGATCTTCGGCcagcagc > 1:3908302/1‑40 (MQ=255) agTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATa > 1:3454589/1‑62 (MQ=255) gTAAAAGATGCCGTAAGCATGATCTTCGGCcagcagca < 1:5151891/38‑1 (MQ=255) gTAAAAGATGCCGTAAGCATGATCTTCGGCcagcagca < 1:5023045/38‑1 (MQ=255) gTAAAAGATGCCGTAAGCATGATCTTCGGCcagcagca < 1:2304844/38‑1 (MQ=255) gTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGt < 1:3997697/42‑1 (MQ=255) gTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGcc > 1:1506570/1‑70 (MQ=255) tAAAAGATGCCGTAAGCATGATCTTCGGCcagcagcag < 1:1887268/38‑1 (MQ=255) tAAAAGATGCCGTAAGCATGATCTTCGGCcagcag < 1:2654600/35‑1 (MQ=255) tAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGt < 1:2759335/41‑1 (MQ=255) tAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGt < 1:2891433/41‑1 (MQ=255) tAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGt < 1:3398288/41‑1 (MQ=255) tAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACa < 1:5138802/62‑1 (MQ=255) aaaaGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGt < 1:815855/40‑1 (MQ=255) aaaaGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGa > 1:184489/1‑70 (MQ=255) aaaaGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGa < 1:3715774/70‑1 (MQ=255) aaaGATGCCGTAAGCATGATCTTCGGCcagcagca > 1:4631440/1‑35 (MQ=255) aaaGATGCCGTAAGCATGATCTTCGGCcagcagca > 1:51199/1‑35 (MQ=255) aaaGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGa > 1:110389/1‑41 (MQ=255) aaaGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGa > 1:908964/1‑69 (MQ=255) aaaGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGa > 1:1571628/1‑69 (MQ=255) aaaGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGa > 1:4838999/1‑69 (MQ=255) aaaGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGa > 1:4021089/1‑69 (MQ=255) aGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAgc < 1:2018120/71‑1 (MQ=255) aTGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGCGTAAGGcc < 1:1874828/50‑1 (MQ=255) tGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGc < 1:167498/39‑1 (MQ=255) tGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGc < 1:4846957/39‑1 (MQ=255) tGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGcgcg < 1:5632986/71‑1 (MQ=255) gCCGTAAGCATGATCTTCGGCCGGCAGCAGGGTGATGCGGGTaa > 1:5216156/1‑44 (MQ=255) gCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTaa > 1:751354/1‑44 (MQ=255) ccGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGAt < 1:3962875/71‑1 (MQ=255) | AAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGAT > minE/2940859‑2940992 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |