BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	313,558	G→A	8.8%	T177T (ACC→ACT)	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	313,558	0	G	A	8.8%	287.4 / 12.5	102	T177T (ACC→ACT)	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand): ref base G (51/42); new base A (2/7); total (53/49)
Fisher's exact test for biased strand distribution p-value = 8.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 109 total aligned reads displayed.

GCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAG  >  minE/313490‑313625

gCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAgg                                                                    <  1:4347586/70‑1 (MQ=255)

     aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGa                                                                 >  1:1387643/1‑68 (MQ=255)

     aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACtt                                                              >  1:1000774/1‑71 (MQ=255)

     aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACt                                                               <  1:4078213/70‑1 (MQ=255)

         ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACttt                                                             <  1:3908390/68‑1 (MQ=255)

         ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                                          >  1:1044453/1‑71 (MQ=255)

         ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                                          >  1:1079468/1‑71 (MQ=255)

         ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                                          >  1:4168834/1‑71 (MQ=255)

          tCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                                         >  1:278007/1‑71 (MQ=255)

           cTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                                         >  1:3060659/1‑70 (MQ=255)

              tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                                        <  1:3958640/68‑1 (MQ=255)

              tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTa                                                       <  1:1234323/69‑1 (MQ=255)

              tACCAATGCGCCTTCCGTCACGTGCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                                        <  1:2560667/68‑1 (MQ=255)

               aCCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGc                                                    >  1:4053291/1‑71 (MQ=255)

                  aaTGCGGCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAg                                                 <  1:4957902/71‑1 (MQ=255)

                    tGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                                         >  1:1280405/1‑61 (MQ=255)

                      cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                              <  1:555686/70‑1 (MQ=255)

                      cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                              >  1:1815974/1‑70 (MQ=255)

                      cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                                             <  1:3291585/71‑1 (MQ=255)

                       gcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                                             <  1:345774/70‑1 (MQ=255)

                         cTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATc                                          >  1:5429045/1‑71 (MQ=255)

                          ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                                                    <  1:5609237/60‑1 (MQ=255)

                          ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                                                    <  1:410310/60‑1 (MQ=255)

                          ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGAt                                           <  1:2698399/69‑1 (MQ=255)

                          ttCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAg                                                 <  1:333139/63‑1 (MQ=255)

                          ttCCGTCACGTTCGACTTACCAATACGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATc                                          >  1:1768711/1‑70 (MQ=255)

                           tcggTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGa                                            >  1:2488414/4‑67 (MQ=255)

                           tcggTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGa                                            >  1:1674694/4‑67 (MQ=255)

                            ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgc                                       >  1:5309105/1‑71 (MQ=255)

                            ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgc                                       >  1:747901/1‑71 (MQ=255)

                             cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                                      >  1:3528495/1‑71 (MQ=255)

                             cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                                      <  1:2819622/71‑1 (MQ=255)

                             cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                                      <  1:2231851/71‑1 (MQ=255)

                              gTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                                            <  1:4627500/48‑1 (MQ=255)

                              gTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                                            <  1:1119593/48‑1 (MQ=255)

                               tCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                                        <  1:5474630/51‑1 (MQ=255)

                               tCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                                    <  1:2370381/71‑1 (MQ=255)

                                cACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGa                                                >  1:105230/1‑58 (MQ=255)

                                cACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAg                                   >  1:1840239/1‑71 (MQ=255)

                                 aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                                             >  1:3201007/1‑60 (MQ=255)

                                 aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGAt                                           >  1:5252971/1‑62 (MQ=255)

                                 aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGt                                  >  1:3249714/1‑71 (MQ=255)

                                 aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACATTGGTGTAAGcc                                                   >  1:378175/1‑54 (MQ=255)

                                    ttCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGc                                                    <  1:4100273/50‑1 (MQ=255)

                                    ttCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                               <  1:5258923/71‑1 (MQ=255)

                                     tCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGAtt                                              <  1:905778/55‑1 (MQ=255)

                                     tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                              >  1:3422879/1‑55 (MQ=255)

                                     tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                              >  1:2593473/1‑55 (MQ=255)

                                     tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                              >  1:3766842/1‑55 (MQ=255)

                                     tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                              >  1:3141002/1‑55 (MQ=255)

                                     tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGa                                            >  1:3952600/1‑57 (MQ=255)

                                      cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                                            >  1:5520484/1‑40 (MQ=255)

                                      cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                                            >  1:28298/1‑40 (MQ=255)

                                      cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                             >  1:717827/1‑71 (MQ=255)

                                         cTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCa                                                  <  1:3053031/47‑1 (MQ=255)

                                         cTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCTCGCAGTTTCAAc                            <  1:3527718/69‑1 (MQ=255)

                                            aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                             >  1:2968328/1‑65 (MQ=255)

                                            aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                             >  1:1506094/1‑65 (MQ=255)

                                            aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAg                       >  1:1059598/1‑71 (MQ=255)

                                              cAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCt                     <  1:3454192/71‑1 (MQ=255)

                                               aaTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCtt                    <  1:5120344/71‑1 (MQ=255)

                                                aTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                               <  1:5623541/59‑1 (MQ=255)

                                                aTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                               <  1:1477636/59‑1 (MQ=255)

                                                aTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                               <  1:4571945/59‑1 (MQ=255)

                                                 tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                                      >  1:1829267/1‑51 (MQ=255)

                                                 tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTc                  <  1:4584586/71‑1 (MQ=255)

                                                 tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGAACcgcga                                     <  1:3967292/52‑2 (MQ=255)

                                                  gCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                             >  1:712816/1‑59 (MQ=255)

                                                  gCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                             >  1:2261863/1‑59 (MQ=255)

                                                  gCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcg                 >  1:5637393/1‑71 (MQ=255)

                                                   cGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGa                                                >  1:3357281/1‑39 (MQ=255)

                                                   cGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGa                                                >  1:5145249/1‑39 (MQ=255)

                                                   cGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCa                        >  1:3694373/1‑63 (MQ=255)

                                                     aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                                    <  1:823128/49‑1 (MQ=255)

                                                     aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                                    <  1:4850801/49‑1 (MQ=255)

                                                     aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                                    <  1:2506763/49‑1 (MQ=255)

                                                     aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccg              <  1:3844898/71‑1 (MQ=255)

                                                      ccGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccgc             <  1:4261138/71‑1 (MQ=255)

                                                      ccGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccg              >  1:159968/1‑70 (MQ=255)

                                                       cGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGATGCGAGCGCTTTCGACTGTGGCTTTtgcggc             <  1:3684844/70‑1 (MQ=255)

                                                       cGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCa            <  1:137215/71‑1 (MQ=255)

                                                        gCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGATGCGAGCGCTTTCGACTGTGGCTTTTGCGGCaa           <  1:2618231/71‑2 (MQ=255)

                                                        gCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccgc             <  1:5635521/69‑1 (MQ=255)

                                                        gCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccgc             <  1:5641863/69‑1 (MQ=255)

                                                        gCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgcc               <  1:4520171/67‑1 (MQ=255)

                                                          tAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccgc             >  1:4573751/1‑67 (MQ=255)

                                                          tAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGt          <  1:4904374/70‑1 (MQ=255)

                                                            aTCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGtt         <  1:2694304/69‑1 (MQ=255)

                                                            aTCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGtt         <  1:700084/69‑1 (MQ=255)

                                                             tCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAg                       <  1:418016/54‑1 (MQ=255)

                                                             tCGGAGAGGTGACTTAGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                             >  1:2007864/1‑48 (MQ=255)

                                                              cGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcg                 >  1:4558197/1‑59 (MQ=255)

                                                               ggAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTa        >  1:2674627/1‑67 (MQ=255)

                                                               ggAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTa        >  1:523444/1‑67 (MQ=255)

                                                                 atagGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCCCCGCAGTTACCgcag  <  1:4828774/69‑1 (MQ=255)

                                                                 agagGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCgcag  <  1:5159677/71‑1 (MQ=255)

                                                                 agagGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCgcag  <  1:2974496/71‑1 (MQ=255)

                                                                 agagGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCgcag  <  1:4566644/71‑1 (MQ=255)

                                                                 agagGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCgcag  <  1:4123146/71‑1 (MQ=255)

                                                                  gagGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCgcag  >  1:2062819/1‑70 (MQ=255)

GCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAG  >  minE/313490‑313625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`