Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,960,652 G→A 12.5% intergenic (+102/+318) yjjV → / ← yjjW predicted DNase/predicted pyruvate formate lyase activating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,960,6520GA12.5% 124.7 / 7.6 48intergenic (+102/+318)yjjV/yjjWpredicted DNase/predicted pyruvate formate lyase activating enzyme
Reads supporting (aligned to +/- strand):  ref base G (24/18);  new base A (4/2);  total (28/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

AATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCT  >  minE/2960592‑2960720
                                                             |                                                                    
aaTGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAgg                                                            <  1:2266448/71‑1 (MQ=39)
   gCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGt                                                         >  1:3815478/1‑71 (MQ=39)
    ccTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGtt                                                        <  1:418397/71‑1 (MQ=39)
    ccTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGtt                                                        <  1:1261968/71‑1 (MQ=39)
      tGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGc                                                           <  1:1348277/66‑1 (MQ=40)
       gATGCGACGCTTACGCGTCTTATCATGCCTACGG‑‑‑GAACCTGACCGTAGGCCGGATAAGGCGTACAcgccg                                                 >  1:1386597/1‑70 (MQ=2)
       gATGCGACGCTTACGCGTCTTATCATGCCTACGG‑‑‑GAACCTGACCGTAGGCCGGATAAGGCGTACAcgccg                                                 >  1:1462730/1‑70 (MQ=2)
        aTGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTTCCGAACCGTAGGCCGGATAAGGCGTTCAcg                                                    <  1:4287881/71‑1 (MQ=255)
        aTGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcg                                                    >  1:1086477/1‑71 (MQ=255)
         tGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgc                                                   <  1:3833668/71‑1 (MQ=255)
           cGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTCAcgccg                                                 <  1:1777616/71‑1 (MQ=2)
             aCGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTGCACGCCGCa                                               <  1:3472681/71‑1 (MQ=255)
                 ttAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgc                                                   <  1:4207551/63‑1 (MQ=255)
                 ttAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                           >  1:1039706/1‑71 (MQ=40)
                   tgcgcgTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCgg                                          <  1:1820923/68‑1 (MQ=2)
                   tgcgcgTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCgg                                          <  1:4959265/68‑1 (MQ=2)
                   aaCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGGATCCgg                                          <  1:741070/70‑1 (MQ=255)
                     cgcgTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATc                                             >  1:2786095/1‑65 (MQ=2)
                     cgcgTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATc                                             >  1:298579/1‑65 (MQ=2)
                     cgcgTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATc                                             >  1:1885465/1‑65 (MQ=2)
                     cgcgTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATc                                             >  1:1474758/1‑65 (MQ=2)
                       cgTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGtt                                     <  1:1296206/71‑1 (MQ=34)
                           ttATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGgcg                                 >  1:5653810/1‑71 (MQ=34)
                            tATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGgcg                                 >  1:3397393/1‑70 (MQ=35)
                             aTCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGgcg                                 <  1:2672720/69‑1 (MQ=35)
                             aTCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGca                               >  1:933658/1‑71 (MQ=34)
                             aTCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGca                               >  1:743241/1‑71 (MQ=34)
                              tCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGcac                              <  1:416404/71‑1 (MQ=34)
                              tCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGca                               >  1:1875457/1‑70 (MQ=35)
                               cATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGcac                              >  1:1753965/1‑70 (MQ=35)
                               cATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGcac                              >  1:505483/1‑70 (MQ=35)
                                aTGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCa                                                      >  1:2605059/1‑45 (MQ=32)
                                 tGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAAt                           >  1:1886977/1‑71 (MQ=34)
                                 tGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAAt                           <  1:4642378/71‑1 (MQ=34)
                                   ccTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgc                                                   >  1:2448657/1‑45 (MQ=38)
                                   ccTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGc                         >  1:5293163/1‑71 (MQ=34)
                                     tACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGca                               <  1:2823031/63‑1 (MQ=35)
                                      aCAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGca                               <  1:2440780/62‑1 (MQ=35)
                                       cAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTg                      <  1:713638/70‑1 (MQ=35)
                                       cAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGAGcac                              >  1:2691461/1‑62 (MQ=25)
                                            ttGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcg                                                    <  1:1972026/35‑1 (MQ=33)
                                            ttGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGa                >  1:4229766/1‑71 (MQ=34)
                                                     aaCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCt            >  1:5218969/1‑67 (MQ=33)
                                                     aaCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCt            >  1:3922553/1‑67 (MQ=33)
                                                     aaCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCt            >  1:3547113/1‑67 (MQ=33)
                                                      aCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATg                   >  1:4508797/1‑59 (MQ=31)
                                                      aCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATg                   >  1:2650947/1‑59 (MQ=31)
                                                           aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCt  >  1:1568240/1‑71 (MQ=39)
                                                             |                                                                    
AATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCT  >  minE/2960592‑2960720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: