BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	313,570	G→A	9.2%	Y173Y (TAC→TAT)	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	313,570	0	G	A	9.2%	274.3 / 12.7	98	Y173Y (TAC→TAT)	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand): ref base G (47/42); new base A (2/7); total (49/49)
Fisher's exact test for biased strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 108 total aligned reads displayed.

TCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTT  >  minE/313500‑313637

tCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                                                     >  1:278007/1‑71 (MQ=255)

tCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                                                     >  1:284897/1‑71 (MQ=255)

 cTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                                                     >  1:3060659/1‑70 (MQ=255)

    tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                                                    <  1:3958640/68‑1 (MQ=255)

    tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTa                                                                   <  1:1234323/69‑1 (MQ=255)

    tACCAATGCGCCTTCCGTCACGTGCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                                                    <  1:2560667/68‑1 (MQ=255)

     aCCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGc                                                                >  1:4053291/1‑71 (MQ=255)

        aaTGCGGCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAg                                                             <  1:4957902/71‑1 (MQ=255)

          tGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                                                     >  1:1280405/1‑61 (MQ=255)

          tGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                                                     >  1:2424949/1‑61 (MQ=255)

            cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                                          >  1:5604312/1‑70 (MQ=255)

            cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                                                         <  1:3291585/71‑1 (MQ=255)

             gcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                                                         <  1:345774/70‑1 (MQ=255)

               cTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATc                                                      >  1:5429045/1‑71 (MQ=255)

                ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                                                                <  1:410310/60‑1 (MQ=255)

                ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                                                                <  1:4492323/60‑1 (MQ=255)

                ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                                                                <  1:5609237/60‑1 (MQ=255)

                ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGAt                                                       <  1:2698399/69‑1 (MQ=255)

                ttCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAg                                                             <  1:333139/63‑1 (MQ=255)

                ttCCGTCACGTTCGACTTACCAATACGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATc                                                      >  1:1768711/1‑70 (MQ=255)

                 tcggTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGa                                                        >  1:1674694/4‑67 (MQ=255)

                 tcggTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGa                                                        >  1:2488414/4‑67 (MQ=255)

                  ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgc                                                   >  1:747901/1‑71 (MQ=255)

                  ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgc                                                   >  1:5309105/1‑71 (MQ=255)

                   cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                                                  <  1:2819622/71‑1 (MQ=255)

                   cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                                                  <  1:2231851/71‑1 (MQ=255)

                   cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                                                  >  1:3528495/1‑71 (MQ=255)

                     tCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                                                    <  1:5474630/51‑1 (MQ=255)

                     tCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                                                <  1:2370381/71‑1 (MQ=255)

                      cACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGa                                                            >  1:105230/1‑58 (MQ=255)

                      cACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAg                                               >  1:1840239/1‑71 (MQ=255)

                       aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                                                         >  1:3201007/1‑60 (MQ=255)

                       aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGAt                                                       >  1:5252971/1‑62 (MQ=255)

                       aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACATTGGTGTAAGcc                                                               >  1:378175/1‑54 (MQ=255)

                          ttCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGc                                                                <  1:4100273/50‑1 (MQ=255)

                          ttCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                                           <  1:5258923/71‑1 (MQ=255)

                           tCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGAtt                                                          <  1:905778/55‑1 (MQ=255)

                           tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                                          >  1:2593473/1‑55 (MQ=255)

                           tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                                          >  1:3141002/1‑55 (MQ=255)

                           tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                                          >  1:3766842/1‑55 (MQ=255)

                           tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                                          >  1:3422879/1‑55 (MQ=255)

                           tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGa                                                        >  1:3952600/1‑57 (MQ=255)

                            cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                                         >  1:717827/1‑71 (MQ=255)

                               cTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCa                                                              <  1:3053031/47‑1 (MQ=255)

                               cTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCTCGCAGTTTCAAc                                        <  1:3527718/69‑1 (MQ=255)

                                  aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                                         >  1:1506094/1‑65 (MQ=255)

                                  aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                                         >  1:2968328/1‑65 (MQ=255)

                                  aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAg                                   >  1:1059598/1‑71 (MQ=255)

                                    cAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCt                                 <  1:3454192/71‑1 (MQ=255)

                                     aaTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCtt                                <  1:5120344/71‑1 (MQ=255)

                                      aTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                                           <  1:5623541/59‑1 (MQ=255)

                                      aTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                                           <  1:4571945/59‑1 (MQ=255)

                                      aTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                                           <  1:1477636/59‑1 (MQ=255)

                                       tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                                                  >  1:1829267/1‑51 (MQ=255)

                                       tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                                         >  1:3440844/1‑60 (MQ=255)

                                       tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTc                              <  1:4584586/71‑1 (MQ=255)

                                       tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGAACcgcga                                                 <  1:3967292/52‑2 (MQ=255)

                                        gCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                                         >  1:2261863/1‑59 (MQ=255)

                                        gCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                                         >  1:712816/1‑59 (MQ=255)

                                        gCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcg                             >  1:5637393/1‑71 (MQ=255)

                                         cGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGa                                                            >  1:5145249/1‑39 (MQ=255)

                                         cGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGa                                                            >  1:3357281/1‑39 (MQ=255)

                                         cGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCa                                    >  1:3694373/1‑63 (MQ=255)

                                           aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                                                <  1:4850801/49‑1 (MQ=255)

                                           aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                                                <  1:2506763/49‑1 (MQ=255)

                                           aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                                                <  1:823128/49‑1 (MQ=255)

                                           aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccg                          <  1:3844898/71‑1 (MQ=255)

                                            ccGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccgc                         <  1:4261138/71‑1 (MQ=255)

                                            ccGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccg                          >  1:159968/1‑70 (MQ=255)

                                             cGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGATGCGAGCGCTTTCGACTGTGGCTTTtgcggc                         <  1:3684844/70‑1 (MQ=255)

                                             cGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCa                        <  1:137215/71‑1 (MQ=255)

                                              gCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccgc                         <  1:5635521/69‑1 (MQ=255)

                                              gCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccgc                         <  1:5641863/69‑1 (MQ=255)

                                              gCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgcc                           <  1:4520171/67‑1 (MQ=255)

                                                tAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccgc                         >  1:4573751/1‑67 (MQ=255)

                                                tAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccgc                         >  1:3958319/1‑67 (MQ=255)

                                                tAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGt                      <  1:4904374/70‑1 (MQ=255)

                                                  aTCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGtt                     <  1:700084/69‑1 (MQ=255)

                                                  aTCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGtt                     <  1:2694304/69‑1 (MQ=255)

                                                   tCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAg                                   <  1:418016/54‑1 (MQ=255)

                                                   tCGGAGAGGTGACTTAGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                                         >  1:2007864/1‑48 (MQ=255)

                                                    cGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcg                             >  1:4558197/1‑59 (MQ=255)

                                                     ggAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTa                    >  1:2674627/1‑67 (MQ=255)

                                                     ggAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTa                    >  1:523444/1‑67 (MQ=255)

                                                       agagGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCgcag              <  1:5159677/71‑1 (MQ=255)

                                                       agagGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCgcag              <  1:4566644/71‑1 (MQ=255)

                                                       agagGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCgcag              <  1:2974496/71‑1 (MQ=255)

                                                        gagGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCgcag              >  1:2062819/1‑70 (MQ=255)

                                                           gTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGttt                                            <  1:1053314/37‑1 (MQ=255)

                                                           gTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGttt                                            <  1:3608245/37‑1 (MQ=255)

                                                           gTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                                           <  1:1313283/38‑1 (MQ=255)

                                                             gACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCg        >  1:5040767/1‑71 (MQ=255)

                                                             gACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCg        >  1:4287956/1‑71 (MQ=255)

                                                             gACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCg        >  1:3447020/1‑71 (MQ=255)

                                                              aCTTTGGTGTAAGCCAGATTGATCCTCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCg        <  1:3390142/70‑1 (MQ=255)

                                                                  tgctgtAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGt   >  1:367355/4‑71 (MQ=255)

                                                                  tGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCttt                               >  1:4552531/1‑43 (MQ=255)

                                                                  tGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCttt                               >  1:4177671/1‑43 (MQ=255)

                                                                  tGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGt   >  1:1085495/1‑71 (MQ=255)

                                                                   ggTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGtt  >  1:2886451/1‑71 (MQ=255)

TCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTT  >  minE/313500‑313637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`