| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 132,202 | A→G | 8.8% | E165E (GAA→GAG) | yadD → | predicted transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 132,202 | 0 | A | G | 8.8% | 216.3 / 8.6 | 79 | E165E (GAA→GAG) | yadD | predicted transposase |
| Reads supporting (aligned to +/- strand): ref base A (32/40); new base G (5/2); total (37/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGT > minE/132132‑132272 | cGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGaa < 1:3473239/71‑1 (MQ=255) cgACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAAc < 1:3049631/70‑1 (MQ=255) gTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATcggcg < 1:3638659/71‑1 (MQ=255) tCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCTTGCAACATcggcgg < 1:3914110/71‑1 (MQ=255) cTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGaaa < 1:1689273/53‑1 (MQ=255) tataACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGAt > 1:4600577/1‑71 (MQ=255) ataACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGAtt > 1:4143045/1‑71 (MQ=255) aCAGTCCTTTCCCGCTGGTGGATATCACCATCACCCCGGATGACGAAATCATGCAACATcg > 1:2266476/1‑61 (MQ=255) aCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCaa > 1:3525250/1‑56 (MQ=255) aCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATcg > 1:541766/1‑61 (MQ=255) aCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATcg > 1:488148/1‑61 (MQ=255) aCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGc < 1:2161053/70‑1 (MQ=255) aCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGc < 1:3522708/70‑1 (MQ=255) cAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGa < 1:1986915/71‑1 (MQ=255) cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc < 1:1800412/71‑1 (MQ=255) tttCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc > 1:648428/1‑70 (MQ=255) ttCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCTACATCGGCGGATTGCGATTCTCg > 1:2903375/1‑70 (MQ=255) tCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa > 1:5084483/1‑71 (MQ=255) cccGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg < 1:4883306/68‑1 (MQ=255) ccGCTGGTGGATATCACCATCACACCGGATGACGAAAt < 1:4520174/38‑1 (MQ=255) ccGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac < 1:4287679/70‑1 (MQ=255) cGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac < 1:3269773/69‑1 (MQ=255) cTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAAc > 1:3978847/1‑43 (MQ=255) cTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta > 1:5320433/1‑69 (MQ=255) cTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta > 1:1291540/1‑69 (MQ=255) gtggATATCACCATCACACCGGATGACGAAATCATGCAACATcggcg < 1:152848/47‑1 (MQ=255) gtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCa < 1:5028866/71‑1 (MQ=255) ggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa < 1:879856/71‑1 (MQ=255) gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa < 1:2104096/71‑1 (MQ=255) gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa < 1:3186170/71‑1 (MQ=255) gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa < 1:5527922/70‑1 (MQ=255) atatCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTAtag < 1:3532925/48‑3 (MQ=255) atatCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa < 1:2659004/70‑1 (MQ=255) taaCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta > 1:3366443/3‑59 (MQ=255) tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGct > 1:1639548/1‑46 (MQ=255) tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa > 1:4331714/1‑70 (MQ=255) tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta > 1:5194850/1‑59 (MQ=255) tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta > 1:3654363/1‑59 (MQ=255) tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta > 1:1715133/1‑59 (MQ=255) tCACAATCACACCGGATGACGAAATCATGCAACATc > 1:1642589/1‑36 (MQ=255) cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata > 1:4456591/1‑71 (MQ=255) cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat > 1:5139991/1‑70 (MQ=255) cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat > 1:3151006/1‑70 (MQ=255) cACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata > 1:137935/1‑71 (MQ=255) aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata < 1:1682564/70‑1 (MQ=255) aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat < 1:5411175/71‑1 (MQ=255) aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCg < 1:4109234/71‑1 (MQ=255) tCACAGCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg < 1:793773/48‑1 (MQ=255) tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg > 1:3622995/1‑39 (MQ=255) tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg > 1:3342140/1‑39 (MQ=255) tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg > 1:1589802/1‑39 (MQ=255) tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg > 1:2371299/1‑39 (MQ=255) tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg > 1:2733784/1‑39 (MQ=255) tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta < 1:1364574/53‑1 (MQ=255) tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc < 1:2811198/71‑1 (MQ=255) tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc < 1:3733406/71‑1 (MQ=255) tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc > 1:1724271/1‑71 (MQ=255) tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc < 1:1990572/71‑1 (MQ=255) tCACACCGGATGACGAAATCATGCAACATCGGAGGATTGCGATTCTCg < 1:3171776/48‑1 (MQ=255) cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa > 1:1119891/1‑49 (MQ=255) cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa > 1:2427586/1‑49 (MQ=255) cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa > 1:283599/1‑49 (MQ=255) cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa > 1:1469273/1‑49 (MQ=255) cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGa > 1:824333/1‑48 (MQ=255) acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc < 1:1456262/70‑1 (MQ=255) acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc < 1:5225852/70‑1 (MQ=255) acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa < 1:1591358/57‑1 (MQ=255) acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa < 1:4922131/57‑1 (MQ=255) cGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc > 1:5603944/1‑71 (MQ=255) cGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc < 1:1982600/71‑1 (MQ=255) cGGATGACGAAATCATGCAACATAGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcg > 1:4314735/1‑70 (MQ=255) ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa < 1:5499548/57‑1 (MQ=255) ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa < 1:4411448/57‑1 (MQ=255) aTGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaat < 1:1765041/52‑2 (MQ=255) tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac < 1:1419189/41‑1 (MQ=255) tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac < 1:3950972/41‑1 (MQ=255) tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac < 1:5469016/41‑1 (MQ=255) tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac < 1:2405323/41‑1 (MQ=255) tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc < 1:5480886/61‑1 (MQ=255) tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACt < 1:5105414/71‑1 (MQ=255) aCGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACtt > 1:4311932/1‑70 (MQ=255) aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt < 1:4346016/71‑1 (MQ=255) | CGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGT > minE/132132‑132272 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |