| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 313,863 | T→C | 17.6% | I76V (ATC→GTC) ‡ | acrA ← | multidrug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 313,863 | 0 | T | C | 17.6% | 194.3 / 33.1 | 91 | I76V (ATC→GTC) ‡ | acrA | multidrug efflux system |
| Reads supporting (aligned to +/- strand): ref base T (42/33); new base C (10/6); total (52/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTG > minE/313793‑313933 | gCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGAt < 1:772659/71‑1 (MQ=255)gCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGAt < 1:261144/71‑1 (MQ=255) aGGATCAATCTGAAAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGTTTCAGGATaa < 1:2256437/71‑1 (MQ=255) tCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATccc > 1:3630095/1‑71 (MQ=255) tCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATcc > 1:88970/1‑70 (MQ=255) tGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAAc > 1:5186021/1‑71 (MQ=255) aTAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTa < 1:472724/67‑1 (MQ=255) aTAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACtt < 1:4302614/71‑1 (MQ=255) aTAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACt < 1:4365912/70‑1 (MQ=255) gagagagaCACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAg < 1:3355624/71‑1 (MQ=255) agagagaCACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAgg < 1:2898845/71‑1 (MQ=255) agaCACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAAt > 1:4562670/1‑52 (MQ=255) agaCACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGc > 1:3336319/1‑57 (MQ=255) acacCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAAc < 1:5298/71‑1 (MQ=255) ccTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGAt < 1:688721/44‑1 (MQ=255) ccTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTg < 1:755252/59‑1 (MQ=255) ccTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTg < 1:320484/59‑1 (MQ=255) ccTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACg < 1:3472635/65‑1 (MQ=255) tGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATaa > 1:2894055/1‑44 (MQ=255) tGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGa > 1:1226219/1‑61 (MQ=255) tGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTc < 1:390887/69‑1 (MQ=255) tGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTc < 1:268844/69‑1 (MQ=255) gCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGaa > 1:3313676/1‑64 (MQ=255) gCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGaa > 1:5434545/1‑64 (MQ=255) cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATaa > 1:403855/1‑42 (MQ=255) cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATaa > 1:5225794/1‑42 (MQ=255) cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTg > 1:4422835/1‑69 (MQ=255) cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTg > 1:3209556/1‑69 (MQ=255) cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTg > 1:1906437/1‑69 (MQ=255) cGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATc > 1:4141687/1‑71 (MQ=255) acatCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACt < 1:4917131/57‑1 (MQ=255) acatCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACt < 1:5261606/57‑1 (MQ=255) aTGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAg < 1:3123355/52‑1 (MQ=255) tGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg < 1:4271291/71‑1 (MQ=255) gTCGCTACCTTCTTTGAAATTACGCTTCAGGATaa > 1:1187704/1‑35 (MQ=255) gTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGc > 1:4953699/1‑41 (MQ=255) gTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGc > 1:4344865/1‑41 (MQ=255) gTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg > 1:4521836/1‑70 (MQ=255) atCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGc < 1:3393494/62‑1 (MQ=255) tCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACgat > 1:2845283/1‑68 (MQ=255) gCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACtt < 1:2650266/56‑1 (MQ=255) gCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAgg > 1:4051084/1‑71 (MQ=255) cTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGc < 1:254615/71‑1 (MQ=255) aCCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt < 1:1228423/65‑1 (MQ=255) aCCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt < 1:5230191/65‑1 (MQ=255) ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCt < 1:991978/35‑1 (MQ=255) ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAAc < 1:2626268/50‑1 (MQ=255) ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAAc < 1:5564794/50‑1 (MQ=255) ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt > 1:1876190/1‑71 (MQ=255) ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt > 1:1869642/1‑71 (MQ=255) ccTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCatt < 1:2516331/71‑3 (MQ=255) ttcttTGAAATTACGCTTCAGGATAATCCCGCTaa > 1:3772504/1‑35 (MQ=255) ttcttTGAAATTACGCTTCAGGATAATCCCGCTAACTTg < 1:5387738/39‑1 (MQ=255) tcttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACt > 1:1237771/1‑48 (MQ=255) cttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACtt > 1:4605907/1‑48 (MQ=255) cttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACtt > 1:3856700/1‑48 (MQ=255) cttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTg > 1:3984667/1‑71 (MQ=255) tttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGc > 1:513687/1‑71 (MQ=255) tttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGc > 1:2760457/1‑71 (MQ=255) ttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATcc < 1:2144178/55‑1 (MQ=255) gtGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCTGCTATACGATAAGCATTGGTGCg < 1:2343094/70‑1 (MQ=255) tGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCgg > 1:1375392/1‑70 (MQ=255) gAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGa < 1:5551224/70‑1 (MQ=255) aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGt > 1:1981234/1‑65 (MQ=255) aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg > 1:1572776/1‑66 (MQ=255) aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg > 1:536100/1‑66 (MQ=255) aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg > 1:3001702/1‑66 (MQ=255) aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCggc > 1:4205515/1‑68 (MQ=255) aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCGGcc < 1:4455268/71‑1 (MQ=255) aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCGGcc > 1:2215143/1‑71 (MQ=255) aaaTTACTCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAc < 1:5693475/70‑1 (MQ=255) aaaTTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAcc > 1:2856905/1‑71 (MQ=255) aTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg > 1:5356662/1‑61 (MQ=255) aTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg > 1:5135467/1‑61 (MQ=255) aTTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCg < 1:2536126/71‑1 (MQ=255) ttACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg < 1:4605918/71‑1 (MQ=255) ttACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg < 1:2171877/71‑1 (MQ=255) aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg > 1:4701667/1‑48 (MQ=255) aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg > 1:4708280/1‑48 (MQ=255) aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg > 1:1544449/1‑48 (MQ=255) aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGa < 1:2308429/70‑1 (MQ=255) gCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGc > 1:139169/1‑71 (MQ=255) cTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCt < 1:1138572/71‑1 (MQ=255) tCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg < 1:2520358/44‑1 (MQ=255) tCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTc > 1:4285273/1‑70 (MQ=255) cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCgg > 1:3156673/1‑71 (MQ=255) cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCgg > 1:723581/1‑71 (MQ=255) cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCg < 1:5147570/70‑1 (MQ=255) aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTa > 1:1272766/1‑44 (MQ=255) aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg > 1:2580969/1‑51 (MQ=255) aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg > 1:887874/1‑51 (MQ=255) aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg > 1:1081018/1‑51 (MQ=255) ggATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGtt > 1:1133632/1‑71 (MQ=255) gATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtg < 1:663073/71‑1 (MQ=255) tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCa > 1:5552838/1‑44 (MQ=255) tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCa > 1:480781/1‑44 (MQ=255) tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg < 1:157456/71‑1 (MQ=255) tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg < 1:4781529/71‑1 (MQ=255) tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg < 1:4601654/71‑1 (MQ=255) tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg < 1:2803890/71‑1 (MQ=255) | GCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTG > minE/313793‑313933 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |