Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	313,864	A→G	17.4%	I75I (ATT→ATC)	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	313,864	0	A	G	17.4%	198.4 / 32.8	92	I75I (ATT→ATC)	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base A (40/36);  new base G (10/6);  total (50/42)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

AGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTG  >  minE/313795‑313933                                                                      |                                                                      aGGATCAATCTGAAAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGTTTCAGGATaa                                                                      <  1:2256437/71‑1 (MQ=255)     tCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATccc                                                                  >  1:3630095/1‑71 (MQ=255)     tCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATcc                                                                   >  1:88970/1‑70 (MQ=255)           tGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAAc                                                            >  1:5186021/1‑71 (MQ=255)             aTAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTa                                                              <  1:472724/67‑1 (MQ=255)             aTAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACtt                                                          <  1:4302614/71‑1 (MQ=255)             aTAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACt                                                           <  1:4365912/70‑1 (MQ=255)                gagagagaCACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAg                                                       <  1:3355624/71‑1 (MQ=255)                 agagagaCACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAgg                                                      <  1:2898845/71‑1 (MQ=255)                     agaCACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAAt                                                                     >  1:4562670/1‑52 (MQ=255)                     agaCACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGc                                                                >  1:3336319/1‑57 (MQ=255)                       acacCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAAc                                                <  1:5298/71‑1 (MQ=255)                          ccTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTg                                                         <  1:320484/59‑1 (MQ=255)                          ccTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTg                                                         <  1:755252/59‑1 (MQ=255)                          ccTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACg                                                   <  1:3472635/65‑1 (MQ=255)                            tGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATaa                                                                      >  1:2894055/1‑44 (MQ=255)                            tGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGa                                                     >  1:1226219/1‑61 (MQ=255)                            tGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTc                                             <  1:390887/69‑1 (MQ=255)                            tGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTc                                             <  1:268844/69‑1 (MQ=255)                             gCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGaa                                                 >  1:3313676/1‑64 (MQ=255)                             gCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGaa                                                 >  1:5434545/1‑64 (MQ=255)                              cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATaa                                                                      >  1:403855/1‑42 (MQ=255)                              cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATaa                                                                      >  1:5225794/1‑42 (MQ=255)                              cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTg                                           >  1:3209556/1‑69 (MQ=255)                              cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTg                                           >  1:4422835/1‑69 (MQ=255)                              cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTg                                           >  1:1906437/1‑69 (MQ=255)                                 cGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATc                                      >  1:4141687/1‑71 (MQ=255)                                   acatCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACt                                               <  1:5261606/57‑1 (MQ=255)                                   acatCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACt                                               <  1:4917131/57‑1 (MQ=255)                                   aTGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAg                                                       <  1:3123355/52‑1 (MQ=255)                                    tGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                   <  1:4271291/71‑1 (MQ=255)                                     gTCGCTACCTTCTTTGAAATTACGCTTCAGGATaa                                                                      >  1:1187704/1‑35 (MQ=255)                                     gTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGc                                                                >  1:4344865/1‑41 (MQ=255)                                     gTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGc                                                                >  1:4953699/1‑41 (MQ=255)                                     gTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                   >  1:4521836/1‑70 (MQ=255)                                     atCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGc                                          <  1:3393494/62‑1 (MQ=255)                                      tCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACgat                                  >  1:2845283/1‑68 (MQ=255)                                        gCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACtt                                              <  1:2650266/56‑1 (MQ=255)                                        gCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAgg                               >  1:4051084/1‑71 (MQ=255)                                         cTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGc                              <  1:254615/71‑1 (MQ=255)                                           aCCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt                                  <  1:5230191/65‑1 (MQ=255)                                           aCCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt                                  <  1:1228423/65‑1 (MQ=255)                                            ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCt                                                               <  1:991978/35‑1 (MQ=255)                                            ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAAc                                                <  1:5564794/50‑1 (MQ=255)                                            ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAAc                                                <  1:2626268/50‑1 (MQ=255)                                            ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt                           >  1:1876190/1‑71 (MQ=255)                                            ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt                           >  1:1869642/1‑71 (MQ=255)                                            ccTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCatt                           <  1:2516331/71‑3 (MQ=255)                                              ttcttTGAAATTACGCTTCAGGATAATCCCGCTaa                                                             >  1:3772504/1‑35 (MQ=255)                                              ttcttTGAAATTACGCTTCAGGATAATCCCGCTAACTTg                                                         <  1:5387738/39‑1 (MQ=255)                                               tcttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACt                                               >  1:1237771/1‑48 (MQ=255)                                                cttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACtt                                              >  1:3856700/1‑48 (MQ=255)                                                cttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACtt                                              >  1:4605907/1‑48 (MQ=255)                                                cttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTg                       >  1:3984667/1‑71 (MQ=255)                                                 tttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGc                      >  1:513687/1‑71 (MQ=255)                                                 tttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGc                      >  1:2760457/1‑71 (MQ=255)                                                  ttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATcc                                     <  1:2144178/55‑1 (MQ=255)                                                  gtGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCTGCTATACGATAAGCATTGGTGCg                     <  1:2343094/70‑1 (MQ=255)                                                   tGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCgg                    >  1:1375392/1‑70 (MQ=255)                                                    gAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGa                    <  1:5551224/70‑1 (MQ=255)                                                     aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGt                        >  1:1981234/1‑65 (MQ=255)                                                     aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                       >  1:1572776/1‑66 (MQ=255)                                                     aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                       >  1:3001702/1‑66 (MQ=255)                                                     aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                       >  1:536100/1‑66 (MQ=255)                                                     aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCggc                   >  1:4205515/1‑68 (MQ=255)                                                     aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCGGcc                  >  1:2215143/1‑71 (MQ=255)                                                     aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCGGcc                  <  1:4455268/71‑1 (MQ=255)                                                     aaaTTACTCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAc                   <  1:5693475/70‑1 (MQ=255)                                                     aaaTTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAcc                  >  1:2856905/1‑71 (MQ=255)                                                       aTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                          >  1:5356662/1‑61 (MQ=255)                                                       aTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                          >  1:5135467/1‑61 (MQ=255)                                                       aTTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCg                <  1:2536126/71‑1 (MQ=255)                                                        ttACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg               <  1:4605918/71‑1 (MQ=255)                                                        ttACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg               <  1:2171877/71‑1 (MQ=255)                                                          aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg                                    >  1:4701667/1‑48 (MQ=255)                                                          aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg                                    >  1:4708280/1‑48 (MQ=255)                                                          aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg                                    >  1:1544449/1‑48 (MQ=255)                                                          aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGa              <  1:2308429/70‑1 (MQ=255)                                                            gCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGc           >  1:139169/1‑71 (MQ=255)                                                             cTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCt          <  1:1138572/71‑1 (MQ=255)                                                               tCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                   <  1:2520358/44‑1 (MQ=255)                                                               tCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTc         >  1:4285273/1‑70 (MQ=255)                                                                cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCgg       >  1:723581/1‑71 (MQ=255)                                                                cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCgg       >  1:3156673/1‑71 (MQ=255)                                                                cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCg        <  1:5147570/70‑1 (MQ=255)                                                                 aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTa                                 >  1:1272766/1‑44 (MQ=255)                                                                 aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                          >  1:887874/1‑51 (MQ=255)                                                                 aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                          >  1:1081018/1‑51 (MQ=255)                                                                 aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                          >  1:2580969/1‑51 (MQ=255)                                                                  ggATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGtt     >  1:1133632/1‑71 (MQ=255)                                                                   gATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtg    <  1:663073/71‑1 (MQ=255)                                                                     tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCa                             >  1:5552838/1‑44 (MQ=255)                                                                     tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCa                             >  1:480781/1‑44 (MQ=255)                                                                     tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg  <  1:157456/71‑1 (MQ=255)                                                                     tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg  <  1:4781529/71‑1 (MQ=255)                                                                     tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg  <  1:4601654/71‑1 (MQ=255)                                                                     tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg  <  1:2803890/71‑1 (MQ=255)                                                                      |                                                                      AGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTG  >  minE/313795‑313933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑