Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	313,891	T→G	19.5%	A66A (GCA→GCC)	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	313,891	0	T	G	19.5%	142.2 / 38.8	82	A66A (GCA→GCC)	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base T (34/32);  new base G (13/3);  total (47/35)
Fisher's exact test for biased strand distribution p-value = 4.71e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGT  >  minE/313824‑313959                                                                    |                                                                     cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTg                                                                     >  1:3209556/1‑69 (MQ=255) cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTg                                                                     >  1:1906437/1‑69 (MQ=255) cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTg                                                                     >  1:4422835/1‑69 (MQ=255)    cGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATc                                                                >  1:4141687/1‑71 (MQ=255)       tGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                                             <  1:4271291/71‑1 (MQ=255)        gTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                                             >  1:4521836/1‑70 (MQ=255)        atCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGc                                                                    <  1:3393494/62‑1 (MQ=255)         tCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACgat                                                            >  1:2845283/1‑68 (MQ=255)           gCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAgg                                                         >  1:4051084/1‑71 (MQ=255)            cTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGc                                                        <  1:254615/71‑1 (MQ=255)              aCCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt                                                            <  1:1228423/65‑1 (MQ=255)              aCCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt                                                            <  1:5230191/65‑1 (MQ=255)               ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt                                                     >  1:1869642/1‑71 (MQ=255)               ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt                                                     >  1:1876190/1‑71 (MQ=255)               ccTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCatt                                                     <  1:2516331/71‑3 (MQ=255)                   cttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTg                                                 >  1:3984667/1‑71 (MQ=255)                    tttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGc                                                >  1:513687/1‑71 (MQ=255)                    tttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGc                                                >  1:2760457/1‑71 (MQ=255)                     ttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATcc                                                               <  1:2144178/55‑1 (MQ=255)                     gtGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCTGCTATACGATAAGCATTGGTGCg                                               <  1:2343094/70‑1 (MQ=255)                      tGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCgg                                              >  1:1375392/1‑70 (MQ=255)                       gAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGa                                              <  1:5551224/70‑1 (MQ=255)                        aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGt                                                  >  1:1981234/1‑65 (MQ=255)                        aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                                                 >  1:536100/1‑66 (MQ=255)                        aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                                                 >  1:1572776/1‑66 (MQ=255)                        aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                                                 >  1:3001702/1‑66 (MQ=255)                        aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCggc                                             >  1:4205515/1‑68 (MQ=255)                        aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCGGcc                                            >  1:2215143/1‑71 (MQ=255)                        aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCGGcc                                            <  1:4455268/71‑1 (MQ=255)                        aaaTTACTCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAc                                             <  1:5693475/70‑1 (MQ=255)                        aaaTTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAcc                                            >  1:2856905/1‑71 (MQ=255)                          aTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                                    >  1:5135467/1‑61 (MQ=255)                          aTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                                    >  1:5356662/1‑61 (MQ=255)                          aTTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCg                                          <  1:2536126/71‑1 (MQ=255)                           ttACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg                                         <  1:2171877/71‑1 (MQ=255)                           ttACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg                                         <  1:4605918/71‑1 (MQ=255)                             aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg                                                              >  1:4708280/1‑48 (MQ=255)                             aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg                                                              >  1:4701667/1‑48 (MQ=255)                             aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg                                                              >  1:1544449/1‑48 (MQ=255)                             aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGa                                        <  1:2308429/70‑1 (MQ=255)                               gCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGc                                     >  1:139169/1‑71 (MQ=255)                                cTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCt                                    <  1:1138572/71‑1 (MQ=255)                                  tCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                                             <  1:2520358/44‑1 (MQ=255)                                  tCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTc                                   >  1:4285273/1‑70 (MQ=255)                                   cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCgg                                 >  1:3156673/1‑71 (MQ=255)                                   cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCgg                                 >  1:723581/1‑71 (MQ=255)                                   cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCg                                  <  1:5147570/70‑1 (MQ=255)                                    aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTa                                                           >  1:1272766/1‑44 (MQ=255)                                    aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                                                    >  1:1081018/1‑51 (MQ=255)                                    aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                                                    >  1:887874/1‑51 (MQ=255)                                    aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                                                    >  1:2580969/1‑51 (MQ=255)                                     ggATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGtt                               >  1:1133632/1‑71 (MQ=255)                                      gATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtg                              <  1:663073/71‑1 (MQ=255)                                        tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCa                                                       >  1:5552838/1‑44 (MQ=255)                                        tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCa                                                       >  1:480781/1‑44 (MQ=255)                                        tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                            <  1:157456/71‑1 (MQ=255)                                        tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                            <  1:4601654/71‑1 (MQ=255)                                        tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                            <  1:4781529/71‑1 (MQ=255)                                        tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                            <  1:2803890/71‑1 (MQ=255)                                          aTCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGa                           <  1:28518/70‑1 (MQ=255)                                          aTCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                                    >  1:3751927/1‑45 (MQ=255)                                          aTCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                                    >  1:1602933/1‑45 (MQ=255)                                          aTCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                                    >  1:180262/1‑45 (MQ=255)                                           tCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTa                                                           >  1:4622318/1‑37 (MQ=255)                                           tCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTa                                                           >  1:999242/1‑37 (MQ=255)                                             ccGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTg                       >  1:4630114/1‑71 (MQ=255)                                               gCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCa                     <  1:5687358/71‑1 (MQ=255)                                                cTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag                    <  1:4744225/71‑1 (MQ=255)                                                cTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag                    <  1:2961360/71‑1 (MQ=255)                                                cTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag                    <  1:2953582/71‑1 (MQ=255)                                                 tAACTTGAGGAGGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                            <  1:4120954/62‑1 (MQ=255)                                                 tAACTTGAGGACGAACTTCTGCGATCCGGTAGGCa                                                       >  1:2529336/1‑35 (MQ=255)                                                 tAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGtt                               >  1:464566/1‑59 (MQ=255)                                                 tAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                            <  1:2541091/62‑1 (MQ=255)                                                 tAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                            <  1:1502636/62‑1 (MQ=255)                                                  aaCTTGAGGACGAACTTCTGCGGTCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCagag                  >  1:5040791/1‑71 (MQ=255)                                                  aaCTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                            <  1:2107542/61‑1 (MQ=255)                                                    cTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGAt                          >  1:4652778/1‑61 (MQ=255)                                                     ttGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                            <  1:1048885/58‑1 (MQ=255)                                                       gAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGAt                          >  1:4345115/1‑58 (MQ=255)                                                          gACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag                    <  1:2579096/61‑1 (MQ=255)                                                                   cTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGt  <  1:3090748/70‑1 (MQ=255)                                                                    |                                                                     CTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGT  >  minE/313824‑313959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑