Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	313,900	G→A	20.5%	Y63Y (TAC→TAT)	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	313,900	0	G	A	20.5%	144.5 / 30.6	73	Y63Y (TAC→TAT)	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base G (26/32);  new base A (12/3);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 2.03e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

TGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCC  >  minE/313830‑313968                                                                       |                                                                     tGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                                                      <  1:4271291/71‑1 (MQ=255)  gTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                                                      >  1:4521836/1‑70 (MQ=255)     gCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAgg                                                                  >  1:4051084/1‑71 (MQ=255)      cTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGc                                                                 <  1:254615/71‑1 (MQ=255)        aCCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt                                                                     <  1:5230191/65‑1 (MQ=255)        aCCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt                                                                     <  1:1228423/65‑1 (MQ=255)         ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt                                                              >  1:1876190/1‑71 (MQ=255)         ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt                                                              >  1:1869642/1‑71 (MQ=255)         ccTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCatt                                                              <  1:2516331/71‑3 (MQ=255)             cttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTg                                                          >  1:3984667/1‑71 (MQ=255)              tttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGc                                                         >  1:513687/1‑71 (MQ=255)              tttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGc                                                         >  1:2760457/1‑71 (MQ=255)               gtGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCTGCTATACGATAAGCATTGGTGCg                                                        <  1:2343094/70‑1 (MQ=255)                tGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCgg                                                       >  1:1375392/1‑70 (MQ=255)                 gAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGa                                                       <  1:5551224/70‑1 (MQ=255)                  aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGt                                                           >  1:1981234/1‑65 (MQ=255)                  aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                                                          >  1:536100/1‑66 (MQ=255)                  aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                                                          >  1:1572776/1‑66 (MQ=255)                  aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                                                          >  1:3001702/1‑66 (MQ=255)                  aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCggc                                                      >  1:4205515/1‑68 (MQ=255)                  aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCGGcc                                                     >  1:2215143/1‑71 (MQ=255)                  aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCGGcc                                                     <  1:4455268/71‑1 (MQ=255)                  aaaTTACTCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAc                                                      <  1:5693475/70‑1 (MQ=255)                  aaaTTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAcc                                                     >  1:2856905/1‑71 (MQ=255)                    aTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                                             >  1:5356662/1‑61 (MQ=255)                    aTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                                             >  1:5135467/1‑61 (MQ=255)                    aTTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCg                                                   <  1:2536126/71‑1 (MQ=255)                     ttACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg                                                  <  1:2171877/71‑1 (MQ=255)                     ttACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg                                                  <  1:4605918/71‑1 (MQ=255)                       aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGa                                                 <  1:2308429/70‑1 (MQ=255)                         gCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGc                                              >  1:139169/1‑71 (MQ=255)                          cTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCt                                             <  1:1138572/71‑1 (MQ=255)                            tCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                                                      <  1:2520358/44‑1 (MQ=255)                            tCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTc                                            >  1:4285273/1‑70 (MQ=255)                             cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCgg                                          >  1:723581/1‑71 (MQ=255)                             cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCgg                                          >  1:3156673/1‑71 (MQ=255)                             cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCg                                           <  1:5147570/70‑1 (MQ=255)                              aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTa                                                                    >  1:1272766/1‑44 (MQ=255)                              aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                                                             >  1:1081018/1‑51 (MQ=255)                              aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                                                             >  1:887874/1‑51 (MQ=255)                              aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                                                             >  1:2580969/1‑51 (MQ=255)                               ggATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGtt                                        >  1:1133632/1‑71 (MQ=255)                                gATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtg                                       <  1:663073/71‑1 (MQ=255)                                  tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCa                                                                >  1:5552838/1‑44 (MQ=255)                                  tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCa                                                                >  1:480781/1‑44 (MQ=255)                                  tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                     <  1:157456/71‑1 (MQ=255)                                  tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                     <  1:2803890/71‑1 (MQ=255)                                  tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                     <  1:4601654/71‑1 (MQ=255)                                  tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                     <  1:4781529/71‑1 (MQ=255)                                    aTCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGa                                    <  1:28518/70‑1 (MQ=255)                                    aTCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                                             >  1:3751927/1‑45 (MQ=255)                                    aTCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                                             >  1:1602933/1‑45 (MQ=255)                                    aTCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                                             >  1:180262/1‑45 (MQ=255)                                     tCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTa                                                                    >  1:999242/1‑37 (MQ=255)                                     tCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTa                                                                    >  1:4622318/1‑37 (MQ=255)                                       ccGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTg                                >  1:4630114/1‑71 (MQ=255)                                         gCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCa                              <  1:5687358/71‑1 (MQ=255)                                          cTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag                             <  1:4744225/71‑1 (MQ=255)                                          cTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag                             <  1:2961360/71‑1 (MQ=255)                                          cTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag                             <  1:2953582/71‑1 (MQ=255)                                           tAACTTGAGGAGGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                     <  1:4120954/62‑1 (MQ=255)                                           tAACTTGAGGACGAACTTCTGCGATCCGGTAGGCa                                                                >  1:2529336/1‑35 (MQ=255)                                           tAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGtt                                        >  1:464566/1‑59 (MQ=255)                                           tAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                     <  1:2541091/62‑1 (MQ=255)                                           tAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                     <  1:1502636/62‑1 (MQ=255)                                            aaCTTGAGGACGAACTTCTGCGGTCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCagag                           >  1:5040791/1‑71 (MQ=255)                                            aaCTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                     <  1:2107542/61‑1 (MQ=255)                                              cTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGAt                                   >  1:4652778/1‑61 (MQ=255)                                               ttGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                     <  1:1048885/58‑1 (MQ=255)                                                 gAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGAt                                   >  1:4345115/1‑58 (MQ=255)                                                    gACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag                             <  1:2579096/61‑1 (MQ=255)                                                             cTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGt           <  1:3090748/70‑1 (MQ=255)                                                               gCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGtttt                 <  1:1925149/62‑1 (MQ=255)                                                                cGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTtac        <  1:4439050/70‑1 (MQ=255)                                                                     cGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGcc  <  1:4939065/71‑1 (MQ=255)                                                                     cGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGcc  <  1:3288105/71‑1 (MQ=255)                                                                       |                                                                     TGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCC  >  minE/313830‑313968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑