| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 325,034 | G→T | 5.3% | S482S (TCG→TCT) | htpG → | molecular chaperone HSP90 family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 325,034 | 0 | G | T | 5.3% | 304.6 / 2.2 | 93 | S482S (TCG→TCT) | htpG | molecular chaperone HSP90 family |
| Reads supporting (aligned to +/- strand): ref base G (41/47); new base T (3/2); total (44/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
CTTTCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAG > minE/324966‑325103 | ctTTCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCgg > 1:414338/1‑70 (MQ=255)ctTTCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgt > 1:4479922/1‑71 (MQ=255) ttCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgtgt > 1:4929981/1‑71 (MQ=255) ttCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgtgt > 1:4750449/1‑71 (MQ=255) cGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTa < 1:3754812/71‑1 (MQ=255) cGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTa < 1:4215588/71‑1 (MQ=255) cGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTa < 1:476906/71‑1 (MQ=255) cGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTa < 1:2172347/71‑1 (MQ=255) aCCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTaaa < 1:1970939/71‑1 (MQ=255) aCCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTa > 1:4703651/1‑69 (MQ=255) ccGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTaaa < 1:4444261/70‑1 (MQ=255) ccGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAg < 1:4233318/71‑1 (MQ=255) gCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGtt < 1:5202696/71‑1 (MQ=255) gCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGtt < 1:2110772/71‑1 (MQ=255) gCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGtt < 1:3011042/71‑1 (MQ=255) cATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgt > 1:1499357/1‑60 (MQ=255) cATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgt > 1:5376689/1‑60 (MQ=255) cATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgt > 1:3767965/1‑60 (MQ=255) tCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCg > 1:1012235/1‑56 (MQ=255) tCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCg > 1:2020001/1‑56 (MQ=255) tCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCg > 1:612703/1‑56 (MQ=255) tCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCg > 1:1591517/1‑56 (MQ=255) tCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTaa < 1:2513710/64‑1 (MQ=255) tCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGAc < 1:4977268/71‑1 (MQ=255) tCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGAc < 1:769878/71‑1 (MQ=255) gATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCgg > 1:3739541/1‑55 (MQ=255) gATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGt > 1:3040560/1‑65 (MQ=255) gATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGa > 1:1891137/1‑68 (MQ=255) gATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGa > 1:3278479/1‑68 (MQ=255) gATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGAc > 1:5078307/1‑69 (MQ=255) gATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGAc > 1:2550211/1‑69 (MQ=255) gATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAAACGTTCCAGTCGGTGTCTAAAGTTGAc > 1:3206269/1‑69 (MQ=255) aGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTc < 1:276366/70‑1 (MQ=255) tGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCg < 1:2076086/48‑1 (MQ=255) tGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCg < 1:3934528/48‑1 (MQ=255) gatgatGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgt > 1:1910171/1‑48 (MQ=255) gatgatGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgt > 1:1940470/1‑48 (MQ=255) gatgatGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgt > 1:1337967/1‑48 (MQ=255) gatgatGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgt > 1:695461/1‑48 (MQ=255) gatgatGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCtt > 1:2007859/1‑70 (MQ=255) gatgatGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTg < 1:5122867/71‑1 (MQ=255) gatgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGa < 1:286094/60‑1 (MQ=255) gatgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGa < 1:1870240/60‑1 (MQ=255) atgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCATTCGGTGTCTAAAGTTGACGAGTCGCTTGaaa < 1:2475267/70‑1 (MQ=255) atgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaaa < 1:4144955/71‑1 (MQ=255) atgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaa < 1:1321471/70‑1 (MQ=255) atgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaa < 1:2494102/70‑1 (MQ=255) atgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaa < 1:5098741/70‑1 (MQ=255) atgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaa < 1:2187931/70‑1 (MQ=255) tgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGt > 1:5666234/1‑60 (MQ=255) tATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaa < 1:3476612/64‑1 (MQ=255) tATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaa < 1:1313241/64‑1 (MQ=255) tCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGtt < 1:4266066/46‑1 (MQ=255) tCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCt > 1:2653310/1‑57 (MQ=255) tCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCt > 1:5380774/1‑57 (MQ=255) ctgactgaGTTCGACGGTAAACCGTTCCAGTCGgtgt < 1:3630192/37‑1 (MQ=255) tgactgaGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGa > 1:5415853/1‑46 (MQ=255) tgactgaGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGa > 1:5101605/1‑46 (MQ=255) tgactgaGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGAc > 1:4050161/1‑71 (MQ=255) actgaGTTCGACGGTAAACCGTTCCAGTCGGTGTCTa < 1:2210341/37‑1 (MQ=255) actgaGTTCGACGGTAAACCGTTCCAGTCGGTGTCTa < 1:2445211/37‑1 (MQ=255) actgaGTTCGACGGTAAACCGTTCCAGTCGGTGTCTa < 1:3260398/37‑1 (MQ=255) ctgaGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaaa < 1:3185125/58‑1 (MQ=255) tgaGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaaa < 1:2888715/57‑1 (MQ=255) aGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGtt > 1:1752657/1‑71 (MQ=255) aGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGtt > 1:2011152/1‑71 (MQ=255) aGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGtt < 1:2116836/71‑1 (MQ=255) gTTCGACTGTAAACCGGTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCGGACGAAGTtg < 1:138475/71‑1 (MQ=255) gTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGtt < 1:279424/37‑1 (MQ=255) gTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGtt > 1:4633751/1‑70 (MQ=255) ttCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtg < 1:551334/70‑1 (MQ=255) tCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtgat > 1:5686805/1‑71 (MQ=255) cGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtgat < 1:1228622/70‑1 (MQ=255) cGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtgat < 1:4056530/70‑1 (MQ=255) cGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtgat < 1:1848283/70‑1 (MQ=255) gACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtgacga < 1:4499850/71‑1 (MQ=255) cGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtgatga > 1:1319304/1‑69 (MQ=255) gTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaaaa < 1:4206105/47‑1 (MQ=255) gTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtgat < 1:4528821/65‑1 (MQ=255) gTAAACCGTTCCAGTCGGTGTCTAAAGCTGACGAGTCGCTTGaaaaa < 1:2295609/47‑1 (MQ=255) tAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtga < 1:2461823/63‑1 (MQ=255) aaaCCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTg > 1:2822566/1‑40 (MQ=255) aaaCCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACt > 1:576699/1‑47 (MQ=255) aaaCCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACg > 1:4614711/1‑55 (MQ=255) aaCCGTTCCAGTCTGTGTCTAAAGTTGACGAGTCGCtg > 1:2888440/1‑37 (MQ=255) aaCCGTTCCAGTCTGTGTCTAAAGTTGACGAGTCGCtg > 1:683448/1‑37 (MQ=255) aaCCGTTCCAGTCTGTGTCTAAAGTTGACGAGTCGCtg > 1:5188630/1‑37 (MQ=255) aaCCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAAc > 1:3608674/1‑45 (MQ=255) aaCCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAAc > 1:5217936/1‑45 (MQ=255) aaCCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAAc > 1:2147599/1‑45 (MQ=255) ccGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaaaa > 1:3917486/1‑42 (MQ=255) ccGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGa > 1:1679360/1‑38 (MQ=255) ccGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGa > 1:3731245/1‑38 (MQ=255) ttCCAGTCTGTGTCTAAAGTTGACGAGTCTCTGGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGa < 1:2285955/67‑1 (MQ=255) ttCCAGTCTGTGTCTAAAGTTGACGAGTCGCTGGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGa < 1:5289291/67‑1 (MQ=255) ttCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAaaga < 1:5071815/71‑1 (MQ=255) cAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGtt < 1:1056041/51‑1 (MQ=255) cAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGc < 1:1479284/71‑1 (MQ=255) aGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTgg > 1:4081845/1‑39 (MQ=255) ggTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGTGAAAGAAGCGgag < 1:4087167/70‑1 (MQ=255) | CTTTCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAG > minE/324966‑325103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |