| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 346,983 | G→A | 6.7% | P210L (CCG→CTG) | ybbO ← | predicted oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 346,983 | 0 | G | A | 6.7% | 171.8 / 2.1 | 59 | P210L (CCG→CTG) | ybbO | predicted oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (31/24); new base A (2/2); total (33/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
TAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGAATGGGACC > minE/346919‑347051 | taaAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATTCCGGGAttt < 1:5110085/71‑1 (MQ=255) aaaGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCAGGATTTTc < 1:3253168/71‑1 (MQ=255) aaaGCATGGCGCACTTTGTCGACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCAGGATTTTc < 1:2764610/71‑1 (MQ=255) aTGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTg > 1:856812/1‑71 (MQ=255) aTGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTg > 1:1547835/1‑71 (MQ=255) cGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGttt < 1:2211133/71‑1 (MQ=255) aCTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTAt > 1:825170/1‑70 (MQ=255) cTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCa > 1:5245117/1‑71 (MQ=255) ttGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACt > 1:5165763/1‑71 (MQ=255) tCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACttt < 1:5533512/70‑1 (MQ=255) caccacCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGttt < 1:4957382/60‑1 (MQ=255) caccacCGCTTCCGGTCCCAACGTAAAGCGG‑‑GGCGATGCCGGGATTTTCGACTGGttt < 1:3085279/58‑1 (MQ=255) accaccGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGt < 1:5480999/71‑1 (MQ=255) caccGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTa > 1:953029/1‑58 (MQ=255) caccGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTc > 1:1247378/1‑70 (MQ=255) accGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGGTTTTCGACTGGTTTATCACTTTGCGTCt > 1:107586/1‑70 (MQ=255) accGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTg > 1:4491382/1‑71 (MQ=255) accGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTg > 1:4345130/1‑71 (MQ=255) gCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGtt < 1:702171/71‑1 (MQ=255) cTTCCGGTCCCAACGTAAAGCGGGCGGCGACGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGtt > 1:1614627/1‑70 (MQ=255) ttCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCg < 1:1676218/43‑1 (MQ=255) tCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTa < 1:238480/51‑1 (MQ=255) cGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACg < 1:2670684/70‑1 (MQ=255) tCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGt > 1:3689610/1‑71 (MQ=255) ccAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCa < 1:2556918/47‑1 (MQ=255) ccAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCa < 1:463969/47‑1 (MQ=255) ccAACGTAAAGCGGG‑GGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCgg > 1:1657934/1‑71 (MQ=255) cAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTc < 1:1354723/69‑1 (MQ=255) aaCGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGt > 1:3970366/1‑53 (MQ=255) aaCGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTCCGt > 1:1182406/1‑53 (MQ=255) aCGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTa < 1:4056097/41‑1 (MQ=255) aCGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTa < 1:2715665/41‑1 (MQ=255) aCGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATc < 1:4501424/43‑1 (MQ=255) aCGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCgg > 1:2013838/1‑69 (MQ=255) cttAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTa < 1:4319471/38‑1 (MQ=255) gTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGa < 1:4523240/70‑1 (MQ=255) gTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGa < 1:5166002/70‑1 (MQ=255) aaaGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGc > 1:3217670/1‑71 (MQ=255) cgggcggCGATGCCAGGATTTTCGACTGGTTTATCACTTTGCGTCTGATTGACGTTGTCGGTGAAGCGa > 1:1345336/1‑69 (MQ=255) cgggcggCGATGCCAGGATTTTCGACTGGTTTATCACTTTGCGTCTGATTGACGTTGTCGGTGAAGCGAg > 1:284547/1‑70 (MQ=255) gggcggCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTa > 1:5082243/1‑71 (MQ=255) cggcgATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTg < 1:228188/47‑1 (MQ=255) cggcgATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGa < 1:1515228/71‑1 (MQ=255) cggcgATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGa < 1:3795054/71‑1 (MQ=255) gATGCCGGGATTTTCGACTGGTTTATCACTTTGCTTCTGGTTGACGTTGTCGGTGaa < 1:4722551/57‑1 (MQ=255) gATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTc > 1:5659257/1‑51 (MQ=255) aTGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGAATgg > 1:3866249/1‑70 (MQ=255) aTGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGAATgg > 1:888177/1‑70 (MQ=255) tGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGAATGGGa > 1:5301489/1‑71 (MQ=255) tGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGAATGGGa > 1:5452601/1‑71 (MQ=255) tGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGAATGGGa > 1:2201149/1‑71 (MQ=255) gCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGtt < 1:2292242/39‑1 (MQ=255) gCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAg > 1:2425501/1‑55 (MQ=255) gCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGAATGGGAc > 1:711306/1‑71 (MQ=255) gCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGAATGGGAc > 1:897082/1‑71 (MQ=255) gCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGAATGGGAc > 1:1126796/1‑71 (MQ=255) gCCGGGATTTTCGACT‑GTTTATCACTTTGCGTCTGGtt > 1:93808/1‑38 (MQ=38) ccGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGAATGGGAcc > 1:5488389/1‑71 (MQ=255) ccGGGATTTTCGACTGGTTTATCACTTAGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGa > 1:4065448/1‑63 (MQ=255) | TAAAAGCATGGCGTACTTTGTCCACCACCGCTTCCGGTCCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGAATGGGACC > minE/346919‑347051 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |