| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 385,066 | T→C | 16.4% | H92H (CAT→CAC) | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 385,066 | 0 | T | C | 16.4% | 173.6 / 25.5 | 79 | H92H (CAT→CAC) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base T (19/47); new base C (5/8); total (24/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
GCCAGCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGG > minE/384997‑385136 | gCCAGCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCAtt < 1:506824/71‑1 (MQ=255) gCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGc < 1:1271483/71‑1 (MQ=255) ctGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGc < 1:565044/70‑1 (MQ=255) cgcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCa < 1:2759067/70‑1 (MQ=255) cgcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAt > 1:4927011/1‑71 (MQ=255) gcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt < 1:2971558/71‑1 (MQ=255) ccATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGc < 1:4333570/66‑1 (MQ=255) ccATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGc < 1:3791880/66‑1 (MQ=255) cATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc < 1:525246/71‑1 (MQ=255) cATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc < 1:3267131/71‑1 (MQ=255) cATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc < 1:1894336/71‑1 (MQ=255) cATAACGACGGGCTGGACTATGTGCCGAAGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc < 1:4955664/71‑1 (MQ=255) aTAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGcc < 1:1730019/71‑1 (MQ=255) tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt < 1:5663818/67‑1 (MQ=255) tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt < 1:5657472/67‑1 (MQ=255) tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt < 1:5153350/67‑1 (MQ=255) tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt < 1:967651/67‑1 (MQ=255) tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCg < 1:2051506/71‑1 (MQ=255) acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCAtt > 1:1304091/1‑54 (MQ=255) acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCAtt > 1:2071143/1‑54 (MQ=255) acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCa > 1:4797923/1‑63 (MQ=255) acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCggc > 1:1702531/1‑70 (MQ=255) acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCgg < 1:1060206/70‑1 (MQ=255) acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCgg < 1:781360/70‑1 (MQ=255) acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATATTGCGGCCATTGCCg > 1:370272/1‑69 (MQ=255) cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg > 1:3004488/1‑71 (MQ=255) cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg < 1:2937430/71‑1 (MQ=255) cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg < 1:2894153/71‑1 (MQ=255) cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg < 1:1969869/71‑1 (MQ=255) cgacgGACTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg > 1:739857/1‑71 (MQ=255) gacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCAtttt > 1:5138426/1‑54 (MQ=255) gacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGc < 1:2977286/71‑1 (MQ=255) acgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCa > 1:2245817/1‑60 (MQ=255) acgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg < 1:1323330/69‑1 (MQ=255) cgGGCTGGACTATGTGCCGACGGACAGGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg < 1:744356/71‑1 (MQ=255) cgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg < 1:5164516/71‑1 (MQ=255) cgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg < 1:5272378/71‑1 (MQ=255) gggCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAgg < 1:19354/71‑1 (MQ=255) gggCTGGACTATGTGCAGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAgg < 1:2840582/71‑1 (MQ=255) cTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTcc < 1:3776807/71‑1 (MQ=255) tGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCAt > 1:1040396/1‑44 (MQ=255) aCTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGa < 1:3179177/59‑1 (MQ=255) aCTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtg < 1:3968233/71‑1 (MQ=255) tATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggt > 1:1513531/1‑71 (MQ=255) tgtgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTg > 1:3319642/1‑41 (MQ=255) tgtgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggtg < 1:2850864/70‑1 (MQ=255) tgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGcc < 1:3224693/44‑1 (MQ=255) tgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGcc < 1:2117544/44‑1 (MQ=255) tgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGcc < 1:1883475/44‑1 (MQ=255) ccGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGcc > 1:489017/1‑71 (MQ=255) cGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCg > 1:4572003/1‑71 (MQ=255) gACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCgg < 1:554019/71‑1 (MQ=255) gACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCgg < 1:1661392/71‑1 (MQ=255) aCGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCa > 1:5182327/1‑40 (MQ=255) ctgACAAGAAAGTGCTGTTCGGGCACCATTTTGCGGCCATTGCCGGAGCAGGGCCGCTGGTGGGGcc < 1:3712220/65‑1 (MQ=255) ggACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtg > 1:2584290/1‑58 (MQ=255) ggACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTAc > 1:1046367/1‑71 (MQ=255) cAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTgg < 1:2712877/71‑1 (MQ=255) aaGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTggc > 1:3787673/1‑71 (MQ=255) gtgtTGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt < 1:1850922/50‑1 (MQ=255) gtgtTGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt < 1:2651637/50‑1 (MQ=255) gTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCa < 1:1289279/71‑1 (MQ=255) gTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCa < 1:1296041/71‑1 (MQ=255) tGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCg < 1:4700197/44‑1 (MQ=255) tGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCg < 1:33002/44‑1 (MQ=255) gttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCgccgcg < 1:1649463/65‑1 (MQ=255) ttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt > 1:4289989/2‑47 (MQ=255) ttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt > 1:2677279/2‑47 (MQ=255) cTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAAt > 1:868934/1‑71 (MQ=255) tGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCa > 1:3884430/1‑67 (MQ=255) tGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAAt < 1:5489501/70‑1 (MQ=255) gTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATg < 1:5612341/70‑1 (MQ=255) gTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATg < 1:5279289/70‑1 (MQ=255) ttggTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGc > 1:230749/3‑71 (MQ=255) cGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGc > 1:2273881/1‑70 (MQ=255) ggTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCt < 1:1631204/70‑1 (MQ=255) gTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGc > 1:204562/1‑68 (MQ=255) cACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGt < 1:1850941/47‑1 (MQ=255) cACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGt < 1:625748/47‑1 (MQ=255) aCCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTg > 1:4949266/1‑71 (MQ=255) aCCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCt < 1:3629173/70‑1 (MQ=255) ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCt < 1:4808523/65‑1 (MQ=255) ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCt < 1:273080/65‑1 (MQ=255) ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGc > 1:3509648/1‑71 (MQ=255) ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCt < 1:647690/62‑1 (MQ=255) ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgg > 1:5385301/1‑71 (MQ=255) ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgg > 1:373704/1‑71 (MQ=255) | GCCAGCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGG > minE/384997‑385136 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |