Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,138 G→C 40.4% G116G (GGG→GGC cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,1380GC40.4% 26.9 / 49.6 47G116G (GGG→GGCcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (12/16);  new base C (7/12);  total (19/28)
Fisher's exact test for biased strand distribution p-value = 7.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCT  >  minE/385074‑385207
                                                                |                                                                     
ccATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATc                                                                 >  1:724685/1‑71 (MQ=255)
      ccGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGgctg                                                           <  1:3752196/71‑1 (MQ=255)
       cGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGTGCAGATGGGCTACCTGCCTGGCACGCTGTGgctgc                                                          <  1:2802760/71‑1 (MQ=255)
           gCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGgatgat                                                                  >  1:3264642/1‑59 (MQ=255)
           gCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGgatgat                                                                  >  1:2519884/1‑59 (MQ=255)
            cAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCt                                                     <  1:1548247/71‑1 (MQ=255)
              ggTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgggg                                                                       <  1:455446/51‑1 (MQ=255)
                tCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGcg                                                     >  1:4242236/1‑66 (MQ=255)
                  cGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTAGCTGGggt                                                <  1:4945212/70‑1 (MQ=255)
                   gCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtgg                                              <  1:2738382/71‑1 (MQ=255)
                    cTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGggtcgt                                             <  1:3437747/71‑1 (MQ=255)
                       gtggGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGgct                                                            <  1:499600/53‑1 (MQ=255)
                       gtggGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTgct                                          >  1:449019/1‑71 (MQ=255)
                       gtggGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTgct                                          >  1:3439234/1‑71 (MQ=255)
                       gtggGTCCGGTTCGCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGgct                                                            <  1:3781756/53‑1 (MQ=255)
                          ggtccGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGc                                       >  1:3274463/4‑71 (MQ=255)
                           ggCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGcc                                      <  1:5507184/71‑1 (MQ=255)
                                gTACTGGCGGCGCAAATGGGCTACCTGCCGGGgatg                                                                    <  1:1668014/36‑1 (MQ=255)
                                gTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCg                                                       <  1:1648172/49‑1 (MQ=255)
                                 tACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtgg                                              <  1:5656408/57‑1 (MQ=255)
                                 tACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGCCTGCTCGCTGGggtgg                                              <  1:3690331/57‑1 (MQ=255)
                                  aCTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTgg                                                   >  1:4122070/1‑51 (MQ=255)
                                  aCTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcg                                >  1:4901219/1‑70 (MQ=255)
                                   cTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTtctc                                         >  1:2271265/1‑60 (MQ=255)
                                     ggcggcGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTtctc                                         >  1:3012489/1‑58 (MQ=255)
                                      gcggcgCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                             >  1:1625793/1‑69 (MQ=255)
                                      gccgcgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcggtgcggt                              <  1:12417/65‑1 (MQ=255)
                                      gccgcgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCcggtgcggt                              <  1:1569900/65‑1 (MQ=255)
                                         gcgcAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAg                          <  1:2642605/69‑1 (MQ=255)
                                          cgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGac                       <  1:4336456/71‑2 (MQ=255)
                                          cgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGac                       <  1:3892571/71‑2 (MQ=255)
                                           gcAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAtt                      >  1:5187398/1‑71 (MQ=255)
                                            cagaTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGActt                     <  1:4055455/68‑1 (MQ=255)
                                            cAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTggg                                                  <  1:4314084/42‑1 (MQ=255)
                                            cAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTggg                                                  <  1:1156919/42‑1 (MQ=255)
                                            cAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                     <  1:1112480/71‑1 (MQ=255)
                                              gaTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGActtta                   >  1:3683789/2‑69 (MQ=255)
                                              gaTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGActtta                   <  1:1917284/70‑3 (MQ=255)
                                              gaTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGActtta                   <  1:5612495/70‑3 (MQ=255)
                                              aaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGtggtgg                                              <  1:4952236/44‑1 (MQ=255)
                                              aaTGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtgg                                              <  1:890215/44‑1 (MQ=255)
                                                tGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATg                 <  1:4973445/71‑1 (MQ=255)
                                                tGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTAGCCGGTGCGGTGCAGGATTTCATg                 >  1:5038970/1‑71 (MQ=255)
                                                     tACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCt            >  1:2429487/1‑71 (MQ=255)
                                                     tACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCt            >  1:268300/1‑71 (MQ=255)
                                                       ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgt          <  1:3102020/71‑1 (MQ=255)
                                                        cTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtt         >  1:4242997/1‑71 (MQ=255)
                                                         tGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcg                                     >  1:5497278/1‑42 (MQ=255)
                                                         tGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcg                                     >  1:3477614/1‑42 (MQ=255)
                                                           ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                     <  1:808785/56‑1 (MQ=255)
                                                           ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt      >  1:4970713/1‑71 (MQ=255)
                                                             ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCa                   >  1:3114377/1‑56 (MQ=255)
                                                             ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCa                   >  1:890358/1‑56 (MQ=255)
                                                              gggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtg                                  <  1:4647609/40‑1 (MQ=255)
                                                              gggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtg                                  <  1:247791/40‑1 (MQ=255)
                                                              gggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtg                                  <  1:4303072/40‑1 (MQ=255)
                                                               ggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCt  <  1:3221073/71‑1 (MQ=255)
                                                               ggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCt  <  1:416204/71‑1 (MQ=255)
                                                                gatgatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCt  <  1:356398/70‑1 (MQ=255)
                                                                |                                                                     
CCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCT  >  minE/385074‑385207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: