| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 385,411 | G→T | 10.0% | G207G (GGG→GGT) | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 385,411 | 0 | G | T | 10.0% | 206.0 / 4.4 | 67 | G207G (GGG→GGT) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base G (27/33); new base T (2/5); total (29/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGCCCTGACTCATAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTT > minE/385341‑385481 | aaGCCCTGACTCATAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATgggg < 1:806619/71‑1 (MQ=255)aaGCCCTGACTCATAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATgggg < 1:1612222/71‑1 (MQ=255) aGCCCTGACTCATAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGa < 1:1156335/71‑1 (MQ=255) cccTGACTCATAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATc < 1:4021655/71‑1 (MQ=255) ccTGACTCATAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCt < 1:3577421/71‑1 (MQ=255) cTGACTCATAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTa > 1:1465581/1‑71 (MQ=255) aCTCATAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCt < 1:335640/67‑1 (MQ=255) aCTCATAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTAc < 1:2109660/69‑1 (MQ=255) cATAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTAc > 1:5350610/1‑66 (MQ=255) cATAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTAc > 1:2360353/1‑66 (MQ=255) aTAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTAc > 1:921728/1‑65 (MQ=255) tAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCt < 1:2931820/71‑1 (MQ=255) tAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCt < 1:2673099/71‑1 (MQ=255) tAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCt < 1:5321417/71‑1 (MQ=255) tAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCt < 1:3757597/71‑1 (MQ=255) gTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTAc > 1:3358542/1‑58 (MQ=255) gTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGc > 1:4831295/1‑71 (MQ=255) tGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCg > 1:2575199/1‑71 (MQ=255) gAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACATGCGCTATCTGCGTCCg < 1:1323712/71‑1 (MQ=255) aCATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTAc > 1:3965388/1‑51 (MQ=255) aCATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTAc > 1:4508118/1‑51 (MQ=255) aCATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTAc > 1:3387429/1‑51 (MQ=255) aCATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCggg < 1:951749/71‑1 (MQ=255) atatACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATgggg < 1:4955988/43‑1 (MQ=255) tataCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCt < 1:3577834/46‑1 (MQ=255) tataCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCg > 1:2122117/1‑71 (MQ=255) tataCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCg < 1:2811345/71‑1 (MQ=255) ataCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTAt > 1:4006322/1‑56 (MQ=255) ataCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCg > 1:59461/1‑70 (MQ=255) taCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCt < 1:3336642/53‑1 (MQ=255) taCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTa > 1:3992845/1‑71 (MQ=255) ccGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTAtt > 1:371778/1‑71 (MQ=255) ccGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTAtt < 1:865078/71‑1 (MQ=255) ccGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTAtt < 1:4153633/71‑1 (MQ=255) gTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGTATCt < 1:4649858/40‑1 (MQ=255) ttGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGc < 1:3186701/71‑1 (MQ=255) cGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGa < 1:3754821/70‑1 (MQ=255) ttCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGt < 1:1014246/60‑1 (MQ=255) cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgt > 1:4635427/1‑71 (MQ=255) cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgt > 1:107078/1‑71 (MQ=255) cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtg < 1:1659745/70‑1 (MQ=255) cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtg > 1:5071817/1‑70 (MQ=255) aCCATTCCGCTGGCGCTGTTTATTGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc > 1:4454511/1‑71 (MQ=255) aCCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCt < 1:3700600/44‑1 (MQ=255) aCCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCt < 1:4454013/44‑1 (MQ=255) aCCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCt < 1:324285/44‑1 (MQ=255) aCCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTc > 1:295969/1‑49 (MQ=255) ttCCGCTGTCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgg < 1:163748/66‑2 (MQ=255) ttCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCt < 1:5526565/36‑1 (MQ=255) ttCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCt < 1:2132766/36‑1 (MQ=255) ttCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCg < 1:2667754/60‑1 (MQ=255) ttCCGCTGGCGCTGTTTATGGGGATCTACCCGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCg < 1:3361179/68‑1 (MQ=255) tCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCa > 1:4031201/1‑71 (MQ=255) tgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTc > 1:4899344/2‑64 (MQ=255) tgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTc > 1:4975636/2‑64 (MQ=255) gcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTGGta < 1:554944/70‑2 (MQ=255) gcgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTc < 1:3730904/70‑1 (MQ=255) cgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTGGtatc < 1:2715536/71‑4 (MQ=255) cgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCgggg > 1:1152620/1‑41 (MQ=255) gcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTgg < 1:2204072/71‑1 (MQ=255) cTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAgtct < 1:4865738/55‑3 (MQ=255) gTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTgg < 1:4519854/68‑1 (MQ=255) gTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAt > 1:814768/1‑71 (MQ=255) gTTTATGGGGATCTACATGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAt > 1:1186824/1‑71 (MQ=255) tttATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGt < 1:2453367/39‑1 (MQ=19) tttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAtt < 1:914747/71‑1 (MQ=255) ttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTa > 1:671109/1‑68 (MQ=255) tATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATc > 1:2601464/1‑58 (MQ=255) tGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt > 1:2479440/1‑55 (MQ=255) tGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTg < 1:2598206/71‑1 (MQ=255) gggATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAg > 1:5550889/1‑43 (MQ=255) gggATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAg > 1:4471752/1‑43 (MQ=255) gggATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAg > 1:956833/1‑43 (MQ=255) gATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAttt < 1:1930464/71‑1 (MQ=255) | AAGCCCTGACTCATAGCCCGTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTT > minE/385341‑385481 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |