| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 385,429 | G→T | 12.8% | L213L (CTG→CTT) ‡ | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 385,429 | 0 | G | T | 12.8% | 184.7 / 9.6 | 62 | L213L (CTG→CTT) ‡ | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base G (29/25); new base T (3/5); total (32/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
GTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGG > minE/385360‑385497 | gTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGc > 1:4831295/1‑71 (MQ=255) tGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCg > 1:2575199/1‑71 (MQ=255) gAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACATGCGCTATCTGCGTCCg < 1:1323712/71‑1 (MQ=255) aCATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCggg < 1:951749/71‑1 (MQ=255) tataCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCg < 1:2811345/71‑1 (MQ=255) tataCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCg > 1:2122117/1‑71 (MQ=255) ataCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCg > 1:59461/1‑70 (MQ=255) taCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTa > 1:3992845/1‑71 (MQ=255) ccGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTAtt < 1:865078/71‑1 (MQ=255) ccGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTAtt > 1:371778/1‑71 (MQ=255) ccGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTAtt < 1:4153633/71‑1 (MQ=255) ttGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGc < 1:3186701/71‑1 (MQ=255) cGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGa < 1:3754821/70‑1 (MQ=255) ttCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGt < 1:1014246/60‑1 (MQ=255) cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgt > 1:107078/1‑71 (MQ=255) cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgt > 1:4635427/1‑71 (MQ=255) cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtg < 1:1659745/70‑1 (MQ=255) cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtg > 1:5071817/1‑70 (MQ=255) aCCATTCCGCTGGCGCTGTTTATTGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc > 1:4454511/1‑71 (MQ=255) aCCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTc > 1:295969/1‑49 (MQ=255) ttCCGCTGTCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgg < 1:163748/66‑2 (MQ=255) ttCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCg < 1:2667754/60‑1 (MQ=255) ttCCGCTGGCGCTGTTTATGGGGATCTACCCGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCg < 1:3361179/68‑1 (MQ=255) tCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCa > 1:4031201/1‑71 (MQ=255) tgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTc > 1:4899344/2‑64 (MQ=255) tgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTc > 1:4975636/2‑64 (MQ=255) gcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTGGta < 1:554944/70‑2 (MQ=255) gcgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTc < 1:3730904/70‑1 (MQ=255) cgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTGGtatc < 1:2715536/71‑4 (MQ=255) cgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCgggg > 1:1152620/1‑41 (MQ=255) gcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTgg < 1:2204072/71‑1 (MQ=255) cTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAgtct < 1:4865738/55‑3 (MQ=255) gTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTgg < 1:4519854/68‑1 (MQ=255) gTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAt > 1:814768/1‑71 (MQ=255) gTTTATGGGGATCTACATGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAt > 1:1186824/1‑71 (MQ=255) tttATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGt < 1:2453367/39‑1 (MQ=19) tttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAtt < 1:914747/71‑1 (MQ=255) ttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTa > 1:671109/1‑68 (MQ=255) tATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATc > 1:2601464/1‑58 (MQ=255) tGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt > 1:2479440/1‑55 (MQ=255) tGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTg < 1:2598206/71‑1 (MQ=255) gggATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAg > 1:5550889/1‑43 (MQ=255) gggATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAg > 1:956833/1‑43 (MQ=255) gggATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAg > 1:4471752/1‑43 (MQ=255) gATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAttt < 1:1930464/71‑1 (MQ=255) aTCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtg < 1:1589641/42‑1 (MQ=255) aTCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTg < 1:1189991/66‑1 (MQ=255) aTCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTg < 1:1950871/66‑1 (MQ=255) tCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAtttt > 1:477107/1‑70 (MQ=255) tCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGaa > 1:862661/1‑38 (MQ=255) cTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc > 1:353490/1‑42 (MQ=255) cTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc > 1:3118638/1‑42 (MQ=255) tACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGtctct < 1:2387922/42‑2 (MQ=255) aCCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTa > 1:2881672/1‑56 (MQ=255) cTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCAt < 1:661134/71‑1 (MQ=255) gcgcTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTa > 1:1937530/1‑71 (MQ=255) tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt > 1:321450/1‑36 (MQ=255) tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAtt < 1:3591105/55‑1 (MQ=255) tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATctct < 1:32309/70‑1 (MQ=255) tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATctct < 1:3178753/70‑1 (MQ=255) cTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATctct > 1:1951846/1‑69 (MQ=255) cTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTgg > 1:815464/1‑71 (MQ=255) | GTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGG > minE/385360‑385497 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |