| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 385,453 | G→C | 7.8% | V221V (GTG→GTC) | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 385,453 | 0 | G | C | 7.8% | 146.6 / 3.1 | 51 | V221V (GTG→GTC) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base G (25/22); new base C (2/2); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
CACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGAAAG > minE/385384‑385515 | cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgt > 1:4635427/1‑71 (MQ=255)cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgt > 1:107078/1‑71 (MQ=255)cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtg > 1:5071817/1‑70 (MQ=255)cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtg < 1:1659745/70‑1 (MQ=255) aCCATTCCGCTGGCGCTGTTTATTGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc > 1:4454511/1‑71 (MQ=255) ttCCGCTGTCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgg < 1:163748/66‑2 (MQ=255) ttCCGCTGGCGCTGTTTATGGGGATCTACCCGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCg < 1:3361179/68‑1 (MQ=255) tCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCa > 1:4031201/1‑71 (MQ=255) tgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTc > 1:4975636/2‑64 (MQ=255) tgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTc > 1:4899344/2‑64 (MQ=255) gcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTGGta < 1:554944/70‑2 (MQ=255) gcgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTc < 1:3730904/70‑1 (MQ=255) cgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTGGtatc < 1:2715536/71‑4 (MQ=255) gcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTgg < 1:2204072/71‑1 (MQ=255) gTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTgg < 1:4519854/68‑1 (MQ=255) gTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAt > 1:814768/1‑71 (MQ=255) gTTTATGGGGATCTACATGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAt > 1:1186824/1‑71 (MQ=255) tttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAtt < 1:914747/71‑1 (MQ=255) ttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTa > 1:671109/1‑68 (MQ=255) tATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATc > 1:2601464/1‑58 (MQ=255) tGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt > 1:2479440/1‑55 (MQ=255) tGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTg < 1:2598206/71‑1 (MQ=255) gATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAttt < 1:1930464/71‑1 (MQ=255) aTCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtg < 1:1589641/42‑1 (MQ=255) aTCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTg < 1:1189991/66‑1 (MQ=255) aTCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTg < 1:1950871/66‑1 (MQ=255) tCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAtttt > 1:477107/1‑70 (MQ=255) cTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc > 1:3118638/1‑42 (MQ=255) cTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc > 1:353490/1‑42 (MQ=255) tACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGtctct < 1:2387922/42‑2 (MQ=255) aCCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTa > 1:2881672/1‑56 (MQ=255) cTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCAt < 1:661134/71‑1 (MQ=255) gcgcTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTa > 1:1937530/1‑71 (MQ=255) tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt > 1:321450/1‑36 (MQ=255) tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAtt < 1:3591105/55‑1 (MQ=255) tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATctct < 1:32309/70‑1 (MQ=255) tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATctct < 1:3178753/70‑1 (MQ=255) cTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATctct > 1:1951846/1‑69 (MQ=255) cTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTgg > 1:815464/1‑71 (MQ=255) cGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTAtct > 1:943857/1‑64 (MQ=255) gTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTggcggc < 1:5626184/71‑1 (MQ=255) cGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTgg > 1:3061450/1‑71 (MQ=255) ggCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTg < 1:1438989/67‑1 (MQ=255) cGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAtt < 1:2619072/42‑1 (MQ=255) cGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTggc > 1:2731161/1‑60 (MQ=255) tATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCa < 1:3305038/70‑1 (MQ=255) ttGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGa > 1:459473/1‑36 (MQ=255) ttGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGa > 1:1824701/1‑36 (MQ=255) ttGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGa > 1:2394634/1‑36 (MQ=255) ttGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGaa < 1:4021191/71‑1 (MQ=255) ttGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGa < 1:5458560/70‑1 (MQ=255) ttGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGa > 1:236611/1‑70 (MQ=255) tGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTggcggc > 1:5110187/1‑58 (MQ=255) tGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTggg < 1:4450582/62‑1 (MQ=255) ggCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGaaa < 1:1217968/70‑1 (MQ=255) ggCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGAAAg > 1:4846007/1‑71 (MQ=255) gCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGAAAg < 1:4370921/70‑1 (MQ=255) aaGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCa > 1:2055610/1‑39 (MQ=255) tgtCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTggcggc < 1:1826430/50‑1 (MQ=255) tgtCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTggcggc < 1:976719/50‑1 (MQ=255) tgtCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGa < 1:4300122/61‑1 (MQ=255) gtCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTggc > 1:947725/1‑46 (MQ=255) gtCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTgg > 1:559394/1‑45 (MQ=255) | CACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGAAAG > minE/385384‑385515 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |