| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 385,804 | G→A | 34.1% | V338V (GTG→GTA) | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 385,804 | 0 | G | A | 34.1% | 106.1 / 86.9 | 105 | V338V (GTG→GTA) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base G (33/33); new base A (24/11); total (61/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.28e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
TGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGC > minE/385735‑385873 | tGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgtgt < 1:381716/71‑1 (MQ=255)tGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgtg > 1:757379/1‑70 (MQ=255) ggCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc > 1:1851656/1‑71 (MQ=255) ggCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc > 1:3297794/1‑71 (MQ=255) gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc < 1:3043332/70‑1 (MQ=255) gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt < 1:208726/71‑1 (MQ=255) gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt > 1:1195516/1‑71 (MQ=255) gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt < 1:3771173/71‑1 (MQ=255) gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt < 1:2961331/71‑1 (MQ=255) cACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTg > 1:1514140/1‑71 (MQ=255) cTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc > 1:3721522/1‑71 (MQ=255) cTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc > 1:1656502/1‑71 (MQ=255) gCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCC‑‑TGGCGCGGTGTCTGGCTTc < 1:2628831/69‑1 (MQ=255) tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc < 1:5280871/60‑1 (MQ=255) gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg < 1:1045122/70‑1 (MQ=255) gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg < 1:4515735/70‑1 (MQ=255) aCCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCAGTGGCGCGGTGTCTgg < 1:3608653/56‑1 (MQ=255) cGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc < 1:4457964/51‑1 (MQ=255) tAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtctt < 1:3370116/71‑1 (MQ=255) tAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtctt < 1:4003777/71‑1 (MQ=255) aaCCTGTTCGCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc > 1:3309006/1‑71 (MQ=255) aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg > 1:1059454/1‑63 (MQ=255) aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc > 1:5359783/1‑71 (MQ=255) cTGTTCCCGTTCCTGTTTTTCACCATCGCCTGTGGCGCGGTGTCTGGCTTc < 1:754599/51‑1 (MQ=255) cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc < 1:1546709/51‑1 (MQ=255) cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc < 1:705027/51‑1 (MQ=255) cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc < 1:423090/51‑1 (MQ=255) cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg < 1:3770457/71‑1 (MQ=255) cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg < 1:4010256/71‑1 (MQ=255) cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGAt > 1:3604457/1‑62 (MQ=255) cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCgg > 1:3630361/1‑71 (MQ=255) cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCgg > 1:344535/1‑71 (MQ=255) tGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg > 1:316871/1‑70 (MQ=255) tGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCtcttccg < 1:379824/69‑3 (MQ=255) tGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCgg > 1:3299243/1‑70 (MQ=255) tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTcc > 1:3721054/1‑51 (MQ=19) tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCa > 1:204728/1‑52 (MQ=25) tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCa > 1:179118/1‑52 (MQ=25) tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCa > 1:4937681/1‑52 (MQ=25) ttCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa > 1:1626343/1‑50 (MQ=255) ttCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa > 1:2207877/1‑50 (MQ=255) ttCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAc > 1:2880985/1‑71 (MQ=255) cccGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTc > 1:1554944/1‑46 (MQ=255) cccGTTCATGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTc > 1:1115606/1‑46 (MQ=255) ccGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCAc < 1:5355415/71‑1 (MQ=255) gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGc > 1:2734362/1‑40 (MQ=37) gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc > 1:201029/1‑71 (MQ=255) gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc > 1:5588902/1‑71 (MQ=255) gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc > 1:4703036/1‑71 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGcc < 1:168692/71‑1 (MQ=255) ttCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGcc < 1:3677951/71‑1 (MQ=255) tCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCtcttc > 1:2926318/1‑58 (MQ=255) ccTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGa > 1:3575214/1‑50 (MQ=255) cTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt > 1:1272292/1‑50 (MQ=255) cTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt > 1:1213713/1‑50 (MQ=255) cTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt > 1:577523/1‑50 (MQ=255) cTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt > 1:4105061/1‑50 (MQ=255) cTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGccaa < 1:345194/70‑3 (MQ=255) cTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGccaa < 1:2874781/70‑3 (MQ=255) tGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttct < 1:2502230/56‑1 (MQ=255) tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATctct < 1:1289056/51‑1 (MQ=255) tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa < 1:1576820/70‑1 (MQ=255) tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa < 1:5116961/70‑1 (MQ=255) tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa < 1:1661703/70‑1 (MQ=255) tCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga < 1:2420229/57‑3 (MQ=255) aCCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTgg > 1:3700686/1‑70 (MQ=255) aCCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGc > 1:4549970/1‑71 (MQ=255) aCCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGc > 1:2658140/1‑71 (MQ=255) aCCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGc > 1:5469831/1‑71 (MQ=255) ccATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga < 1:1188041/54‑3 (MQ=255) ccATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGct < 1:3375521/71‑2 (MQ=255) tCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTgg < 1:4625930/66‑1 (MQ=255) tCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaac > 1:1952477/1‑71 (MQ=255) tCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaac > 1:5309445/1‑71 (MQ=255) cGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaat > 1:1707696/1‑69 (MQ=255) gCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatg > 1:5027682/1‑68 (MQ=255) gCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatg < 1:5170441/70‑3 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:2474919/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:2238044/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:4227084/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:3113432/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:133727/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcg > 1:3418768/1‑66 (MQ=255) cTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAg > 1:2125439/1‑71 (MQ=255) tgGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaaacc < 1:89464/70‑4 (MQ=255) tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa > 1:4199975/1‑53 (MQ=255) tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa > 1:3948685/1‑53 (MQ=255) tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa > 1:2217885/1‑53 (MQ=255) tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa > 1:2359027/1‑53 (MQ=255) tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa > 1:5612503/1‑53 (MQ=255) ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATg > 1:4360710/1‑54 (MQ=255) ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATg > 1:5336243/1‑54 (MQ=255) ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATg > 1:2834309/1‑54 (MQ=255) ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTT‑AGGGACCACGCCGAAGATg > 1:2771463/1‑53 (MQ=255) gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaa > 1:5412112/3‑61 (MQ=255) gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaa > 1:3497235/3‑61 (MQ=255) gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaa > 1:36651/3‑61 (MQ=255) gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGc > 1:3204708/3‑58 (MQ=255) tgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:4439594/57‑1 (MQ=255) tgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGc < 1:510735/71‑1 (MQ=255) | TGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGC > minE/385735‑385873 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |