Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,831 T→C 38.0% S347S (TCT→TCC cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,8310TC38.0% 71.0 / 90.8 94S347S (TCT→TCCcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base T (31/27);  new base C (21/14);  total (53/41)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAAT  >  minE/385763‑385899
                                                                    |                                                                    
cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg                                                                    <  1:4010256/71‑1 (MQ=255)
cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg                                                                    <  1:3770457/71‑1 (MQ=255)
cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCgg                                                                    >  1:3630361/1‑71 (MQ=255)
cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCgg                                                                    >  1:344535/1‑71 (MQ=255)
 tGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCgg                                                                    >  1:3299243/1‑70 (MQ=255)
   ttCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAc                                                                 >  1:2880985/1‑71 (MQ=255)
    tcgcGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAc                                                                 >  1:2441857/4‑70 (MQ=255)
      ccGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCAc                                                              <  1:5355415/71‑1 (MQ=255)
        gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc                                                            >  1:5588902/1‑71 (MQ=255)
        gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc                                                            >  1:4703036/1‑71 (MQ=255)
        gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc                                                            >  1:201029/1‑71 (MQ=255)
         ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGcc                                                           <  1:168692/71‑1 (MQ=255)
         ttCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGcc                                                           <  1:3677951/71‑1 (MQ=255)
            cTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGccaa                                                         <  1:2874781/70‑3 (MQ=255)
            cTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGccaa                                                         <  1:345194/70‑3 (MQ=255)
             tGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttct                                                                      <  1:2502230/56‑1 (MQ=255)
               tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                      <  1:5116961/70‑1 (MQ=255)
               tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                      <  1:1576820/70‑1 (MQ=255)
               tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                      <  1:1661703/70‑1 (MQ=255)
                   tCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga                                                               <  1:2420229/57‑3 (MQ=255)
                     aCCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGc                                               >  1:4549970/1‑71 (MQ=255)
                     aCCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGc                                               >  1:5469831/1‑71 (MQ=255)
                     aCCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGc                                               >  1:2658140/1‑71 (MQ=255)
                      ccATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga                                                               <  1:1188041/54‑3 (MQ=255)
                      ccATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGct                                              <  1:3375521/71‑2 (MQ=255)
                         tCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTgg                                                <  1:4625930/66‑1 (MQ=255)
                         tCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaac                                           >  1:5309445/1‑71 (MQ=255)
                          cGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaat                                           >  1:1707696/1‑69 (MQ=255)
                           gCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatg                                          <  1:5170441/70‑3 (MQ=255)
                           gCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatg                                          >  1:5027682/1‑68 (MQ=255)
                           gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                          <  1:3113432/70‑1 (MQ=255)
                           gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                          <  1:4227084/70‑1 (MQ=255)
                           gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                          <  1:133727/70‑1 (MQ=255)
                           gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                          <  1:2474919/70‑1 (MQ=255)
                           gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                          <  1:2238044/70‑1 (MQ=255)
                           gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcg                                              >  1:3418768/1‑66 (MQ=255)
                            ccTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatga                                         >  1:4632422/1‑70 (MQ=255)
                            ccTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatga                                         >  1:1528704/1‑70 (MQ=255)
                             cTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAg                                       >  1:2125439/1‑71 (MQ=255)
                                tgGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaaacc                                     <  1:89464/70‑4 (MQ=255)
                                tgGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGct                                              <  1:1943278/61‑2 (MQ=255)
                                tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                      >  1:3948685/1‑53 (MQ=255)
                                tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                      >  1:5612503/1‑53 (MQ=255)
                                tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                      >  1:4199975/1‑53 (MQ=255)
                                tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                      >  1:2359027/1‑53 (MQ=255)
                                tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                      >  1:2217885/1‑53 (MQ=255)
                                 ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATg                                                    >  1:2834309/1‑54 (MQ=255)
                                 ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATg                                                    >  1:5336243/1‑54 (MQ=255)
                                 ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATg                                                    >  1:4360710/1‑54 (MQ=255)
                                 ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTAGGGACCACGCCGAAGATg                                                    >  1:2771463/1‑53 (MQ=255)
                                  gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaa                                            >  1:3497235/3‑61 (MQ=255)
                                  gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaa                                            >  1:5412112/3‑61 (MQ=255)
                                  gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaa                                            >  1:36651/3‑61 (MQ=255)
                                  gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGc                                               >  1:3204708/3‑58 (MQ=255)
                                        tgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                          <  1:4439594/57‑1 (MQ=255)
                                        tgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                          <  1:2946652/57‑1 (MQ=255)
                                        tgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGc                            <  1:510735/71‑1 (MQ=255)
                                          tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                          <  1:1964989/55‑1 (MQ=255)
                                          tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt                          >  1:4033926/1‑71 (MQ=255)
                                          tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt                          <  1:3530569/71‑1 (MQ=255)
                                          tCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaat                                           <  1:256970/54‑2 (MQ=255)
                                            tGGCTTCCCTGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTa                        >  1:4829790/1‑71 (MQ=255)
                                            tGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTa                        >  1:383174/1‑71 (MQ=255)
                                            tGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaaac                                      >  1:3451681/1‑55 (MQ=255)
                                              tcTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGAGAACGAAGGGCAGGCGTGATTTATc                      >  1:1509315/2‑71 (MQ=255)
                                              gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCttt                         <  1:4386772/68‑1 (MQ=255)
                                              gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCttt                         <  1:2768365/68‑1 (MQ=255)
                                              gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATc                      >  1:889385/1‑71 (MQ=255)
                                              gCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaa                                            >  1:3400887/1‑49 (MQ=255)
                                                  ccATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                          <  1:4855209/47‑1 (MQ=255)
                                                   cATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTa                 <  1:385124/71‑1 (MQ=255)
                                                   cATGCGCTGATCTCTTCTGGGACCACGCAGAAGATGCTGGCGAACGAAGGGCAGGc                                >  1:4279267/1‑56 (MQ=255)
                                                    acgcgcTGATCTCTTCCGGTACGACGCCAAAACTGCTGGCTAATGAAACCGACGCGCGTTTCATCGGCTAc                <  1:3511640/69‑1 (MQ=255)
                                                    acgcgcTGATCTCTTCCGGTACGACGCCAAAACTGCTGGCTAATGAAACCGACGCGCGTTTCATCGGCTAc                <  1:3148854/69‑1 (MQ=255)
                                                    aTGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTAc                <  1:5662606/71‑1 (MQ=255)
                                                      gcgcTGATCTCTTCCGGTACGACGCCAAAACTGCTGGCTAATGAAACCGACGCGCGTTTCATCGGCTACgg              >  1:1947638/1‑71 (MQ=255)
                                                       cgcTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTAt                       >  1:1328776/1‑61 (MQ=255)
                                                       cgcTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACgg              >  1:516288/1‑70 (MQ=255)
                                                       cgcTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGt             >  1:1336817/1‑71 (MQ=255)
                                                         cTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg           <  1:4844150/71‑1 (MQ=255)
                                                         cTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg           <  1:1495428/71‑1 (MQ=255)
                                                         cTGATCTCTTCCGGTACGACGCCAAAACTGCTGGCTAATGAAACCGACGCGCGTTTCATCGGCTACggcgc           <  1:4059700/71‑4 (MQ=255)
                                                          tGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg           <  1:3426164/70‑1 (MQ=255)
                                                           gATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacgaa                                        >  1:4492211/1‑40 (MQ=255)
                                                           gATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacgaa                                        >  1:50421/1‑40 (MQ=255)
                                                           gATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacgaa                                        >  1:4415187/1‑40 (MQ=255)
                                                            aTCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGAt        >  1:3010674/1‑71 (MQ=255)
                                                              ctctTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACgg              >  1:3112958/1‑63 (MQ=255)
                                                              ctctTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt      >  1:1357813/1‑71 (MQ=255)
                                                               tcttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt                          <  1:4180707/50‑1 (MQ=255)
                                                               tcttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt                          <  1:1965076/50‑1 (MQ=255)
                                                                cttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt      >  1:4686747/1‑69 (MQ=255)
                                                                cttctGCGACCACGCCGAAGATGCTGGAGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt      >  1:2410232/1‑69 (MQ=255)
                                                                  tctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt     <  1:2364841/68‑1 (MQ=255)
                                                                   ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa    <  1:2013463/68‑1 (MQ=255)
                                                                   ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa    <  1:2760664/68‑1 (MQ=255)
                                                                   ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa    <  1:4899299/68‑1 (MQ=255)
                                                                   ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa    <  1:3034470/68‑1 (MQ=255)
                                                                   ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa    <  1:4011975/68‑1 (MQ=255)
                                                                   ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAAt  >  1:1746415/1‑70 (MQ=255)
                                                                    |                                                                    
CTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAAT  >  minE/385763‑385899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: