| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 5,624 | C→T | 22.9% | intergenic (+94/+59) | yaaX → / ← yaaA | hypothetical protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 5,624 | 0 | C | T | 22.9% | 178.2 / 48.3 | 96 | intergenic (+94/+59) | yaaX/yaaA | hypothetical protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (47/27); new base T (10/12); total (57/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01 | |||||||||||
CGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTG > minE/5554‑5691 | cGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATc > 1:4198018/1‑71 (MQ=255) gCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTg > 1:1033395/1‑71 (MQ=255) cATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTg > 1:3429588/1‑70 (MQ=255) ttCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCt < 1:4489936/67‑1 (MQ=255) tCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCAt > 1:1566045/1‑70 (MQ=255) aGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCt > 1:626255/1‑71 (MQ=255) gATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAg > 1:2140600/1‑71 (MQ=255) aTGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAgg > 1:2157516/1‑71 (MQ=255) tGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAgg > 1:3454739/1‑70 (MQ=255) cGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAg > 1:4258947/1‑71 (MQ=255) cGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAg > 1:4075143/1‑71 (MQ=255) cGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAg < 1:5461251/71‑1 (MQ=255) gACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAg > 1:3075199/1‑70 (MQ=255) cGCTGGCGCGTCTTATCAGTCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGAt < 1:2522751/71‑1 (MQ=255) tGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTg < 1:4933170/48‑1 (MQ=255) gcgcGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCa > 1:4682794/1‑48 (MQ=255) gcgcGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAgg < 1:2819361/70‑1 (MQ=255) cgTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGtt < 1:2253435/71‑1 (MQ=255) tCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATa < 1:1069708/62‑1 (MQ=255) ttATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTg > 1:467451/1‑49 (MQ=255) tATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGt > 1:5188918/1‑49 (MQ=255) tATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCAcgc > 1:3893917/1‑71 (MQ=255) aGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCAcgccgc < 1:2775898/70‑1 (MQ=255) aGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCAcgccgc < 1:2229800/70‑1 (MQ=255) aGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCa > 1:4362773/1‑71 (MQ=255) gCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCa < 1:3846967/62‑1 (MQ=255) cTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATcc < 1:3393367/70‑1 (MQ=255) tACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATa < 1:5540013/50‑1 (MQ=255) tACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGG > 1:5297533‑M1/1‑71 (MQ=255) tACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGG > 1:3953771‑M1/1‑71 (MQ=255) aCGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGG > 1:3297847‑M1/1‑70 (MQ=255) cGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCA > 1:3059320‑M1/1‑71 (MQ=255) cGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCA > 1:153691‑M1/1‑71 (MQ=255) cGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCA > 1:2783007‑M1/1‑71 (MQ=255) gTTAATTCTGCAATATATTGAATCTGCATGCTTTTg > 1:1939379/1‑36 (MQ=255) gTTAATTCTGCAATATATTGAATCTGCATGCTTTTg > 1:2783382/1‑36 (MQ=255) gTTAATTCTGCAATATATTGAATCTGCATGCTTTTg > 1:3526172/1‑36 (MQ=255) ttAATTCTGCAATATATTGAATCTGCATGCTTTTg < 1:3792198/35‑1 (MQ=255) ttAATTCTGCAATATATTGAATCTGCATGCTTTTGTa > 1:2543164/1‑37 (MQ=255) ttAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCAtt > 1:3747856/1‑71 (MQ=255) ttAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCAtt > 1:2146278/1‑71 (MQ=255) ttAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCAtt < 1:1288116/71‑1 (MQ=255) ttAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCAtt < 1:4113015/71‑1 (MQ=255) ttAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCAtt < 1:3994012/71‑1 (MQ=255) ttAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCAtt > 1:2323434/1‑71 (MQ=255) ttAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCAtt > 1:1654681/1‑71 (MQ=255) ttAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCAtt < 1:1825340/71‑1 (MQ=255) tAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCAtt > 1:1791558/1‑70 (MQ=255) aaTTCTGCAATATATTGAATCTGCATGCTTTTGTa > 1:154/1‑35 (MQ=255) aaTTCTGCAATATATTGAATCTGCATGCTTTTGTa > 1:2031531/1‑35 (MQ=255) aaTTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAgg > 1:344469/1‑41 (MQ=255) aaTTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAgg > 1:3636197/1‑41 (MQ=255) aaTTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTg > 1:5380938/1‑70 (MQ=255) aaTTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGa > 1:5387744/1‑71 (MQ=255) aTTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGC < 1:1566591‑M1/65‑1 (MQ=255) ttCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTc > 1:4311763/1‑50 (MQ=255) ttCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCAcgcc < 1:79045/55‑1 (MQ=255) ttCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCAcgcc < 1:2981545/55‑1 (MQ=255) ttCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATc > 1:427967/1‑60 (MQ=255) cTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATaa > 1:3338352/1‑41 (MQ=255) cTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATaa > 1:5620756/1‑41 (MQ=255) cTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATaa > 1:691059/1‑41 (MQ=255) tGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaataa < 1:162167‑M1/69‑7 (MQ=255) tGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaataa < 1:5640649‑M1/69‑7 (MQ=255) tGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaat < 1:1857813‑M1/67‑5 (MQ=255) gCAATATATTGAATCTGCATGCTTTTGTAGGCAGGAt < 1:388539/37‑1 (MQ=255) cAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaataaagcg < 1:3860884‑M1/71‑11 (MQ=255) cAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaataaagcg > 1:5612008‑M1/1‑61 (MQ=255) cAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaataaa < 1:2088042‑M1/68‑8 (MQ=255) cAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaataaa < 1:5534453‑M1/68‑8 (MQ=255) aaTATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaataaagcgc < 1:4347374‑M1/71‑12 (MQ=255) atatatTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaataaagcgca < 1:4381488‑M1/71‑13 (MQ=255) atatatTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaat < 1:3884668‑M1/63‑5 (MQ=255) atatatTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGtt < 1:1848515/42‑1 (MQ=255) atatatTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGtt < 1:3812902/42‑1 (MQ=255) tatatTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATga > 1:2398733‑M1/1‑58 (MQ=255) tatatTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATga > 1:5604673‑M1/1‑58 (MQ=255) tatatTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCa < 1:110977/66‑1 (MQ=255) tatatTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCa < 1:4012164/66‑1 (MQ=255) tatatTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACt > 1:284536/1‑70 (MQ=255) atatTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGtt < 1:1638927/40‑1 (MQ=255) atatTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGtt < 1:2689296/40‑1 (MQ=255) atatTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCa > 1:1252321/1‑42 (MQ=255) atatTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCa > 1:3930461/1‑42 (MQ=255) tatTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaataaagcgcactc < 1:2571599‑M1/71‑16 (MQ=255) tatTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaataaagcgcact > 1:1579168‑M1/1‑56 (MQ=255) tatTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaataaagcgcact > 1:1537235‑M1/1‑56 (MQ=255) tatTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaataaagcgcact > 1:637586‑M1/1‑56 (MQ=255) tatTGAATCTGCATGCTTTTGTAGGAAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTaa > 1:5696364/1‑71 (MQ=255) ttGAATTTGCGTGCTTTTGTAGGCAGGTTAAGGCGTTCACGCCGCATCCGGCATgaataaagcgca > 1:579422‑M1/1‑54 (MQ=255) ttGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAAc > 1:1645072/1‑70 (MQ=255) tGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaataaagcgcactcaa > 1:526587‑M1/1‑53 (MQ=255) tGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaataaagcgcactcaa > 1:1531146‑M1/1‑53 (MQ=255) aaTTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaataaagcgcactcaacaa > 1:4185550‑M1/1‑51 (MQ=255) aaTTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaata < 1:4928258‑M1/56‑6 (MQ=255) aaTTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATgaata < 1:1233799‑M1/56‑6 (MQ=255) aaTCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACgctg > 1:3503473/1‑71 (MQ=255) | CGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTG > minE/5554‑5691 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |