| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 385,974 | G→C | 11.3% | S395T (AGC→ACC) | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 385,974 | 0 | G | C | 11.3% | 114.4 / 6.2 | 44 | S395T (AGC→ACC) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base G (12/27); new base C (3/2); total (15/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-01 | |||||||||||
CTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAG > minE/385906‑386043 | cTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGcc > 1:144419/1‑71 (MQ=255)cTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACAccc > 1:5577617/1‑71 (MQ=255) tCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCg > 1:1968933/1‑71 (MQ=255) cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc > 1:4800594/1‑69 (MQ=255) cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc < 1:5523451/69‑1 (MQ=255) cGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc > 1:2396515/1‑70 (MQ=255) cGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc > 1:1954198/1‑70 (MQ=255) cGTGGCGATTACGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc < 1:1744653/69‑1 (MQ=255) gCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc < 1:1177082/67‑2 (MQ=255) gCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc < 1:1170488/67‑2 (MQ=255) aTTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGt < 1:5356536/71‑1 (MQ=255) tATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACAccc > 1:2232338/1‑59 (MQ=255) tATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACAccc > 1:122627/1‑59 (MQ=255) tGGCGCTGGTTTCCGCCTGTATCATCGAGCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCt < 1:2370925/70‑1 (MQ=255) cTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATg > 1:4033427/1‑57 (MQ=255) ggTTTCCGCCTGTATCATCGATCCTGGCGTGTATTTTGCCATGAACAGccc < 1:46340/51‑1 (MQ=255) ggTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc < 1:1721911/51‑1 (MQ=255) ccTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGAcg > 1:3031944/1‑71 (MQ=255) tATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATg < 1:764686/43‑1 (MQ=255) atcatcGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATg < 1:3170218/42‑1 (MQ=255) tcatcGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGAt > 1:3087739/1‑71 (MQ=255) tCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCg < 1:3520844/71‑1 (MQ=255) tCCGGGCGTGTATTTTACCATTAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCg < 1:1715959/71‑1 (MQ=255) ccGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGc > 1:5260507/1‑71 (MQ=255) cGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCt < 1:4650254/71‑1 (MQ=255) ggCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTc < 1:336755/71‑1 (MQ=255) gCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCt < 1:3744493/71‑1 (MQ=255) cGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTg < 1:3751628/71‑1 (MQ=255) cGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTg < 1:48219/71‑1 (MQ=255) tATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCcgc > 1:3079273/1‑71 (MQ=255) tATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCcg > 1:963691/1‑70 (MQ=255) ttttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGTCGGATGTGGTCGCTTCTGCcgcg < 1:1938472/70‑1 (MQ=255) ttttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGc < 1:2033383/60‑1 (MQ=255) ttttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCcgcg < 1:365381/70‑1 (MQ=255) ttttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCcgcg > 1:19775/1‑70 (MQ=255) ttttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCcgcg < 1:4090815/70‑1 (MQ=255) ttttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCgcgc < 1:5174180/71‑1 (MQ=255) ttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAg > 1:3913300/1‑71 (MQ=255) ccATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAggtg < 1:3772223/71‑1 (MQ=255) ccATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAggt < 1:213990/70‑1 (MQ=255) ccATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAggt < 1:3610177/70‑1 (MQ=255) aTGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAggtggt < 1:478261/71‑1 (MQ=255) aTGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAggtggt < 1:3162243/71‑1 (MQ=255) tGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAggtggt > 1:2724767/1‑70 (MQ=255) tGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGgtggtg < 1:216487/71‑1 (MQ=255) aGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAg > 1:4632892/1‑71 (MQ=255) aGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAg < 1:3763759/71‑1 (MQ=255) gCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCa < 1:2846716/58‑1 (MQ=255) | CTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAG > minE/385906‑386043 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |