| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 386,215 | C→T | 6.8% | A475A (GCC→GCT) ‡ | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 386,215 | 0 | C | T | 6.8% | 301.8 / 7.8 | 102 | A475A (GCC→GCT) ‡ | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base C (52/43); new base T (3/4); total (55/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
GGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGC > minE/386146‑386285 | ggggATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCa > 1:1751017/1‑71 (MQ=255) tGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttg < 1:1016504/71‑1 (MQ=255) tGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttg < 1:4429443/71‑1 (MQ=255) ggCCTACATTCTGCACGGCGCGCTGGGCGGCTTGATGGATGTGGCGTTCTGGTATCACTTCGCCAtttt > 1:4882563/1‑69 (MQ=255) ggCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCAtttt > 1:2253432/1‑69 (MQ=255) ggCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttgt > 1:1094609/1‑71 (MQ=255) ggCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttgt > 1:309098/1‑71 (MQ=255) ggCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttgt > 1:3278328/1‑71 (MQ=255) ccTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttgttt > 1:1001011/1‑71 (MQ=255) ccTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttgttt > 1:4796762/1‑71 (MQ=255) cTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttgtttg < 1:240902/71‑1 (MQ=255) cTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttgttt > 1:3469030/1‑70 (MQ=255) tACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttg < 1:3400997/66‑1 (MQ=255) cATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAg > 1:1375286/1‑71 (MQ=255) cATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAg > 1:4974094/1‑71 (MQ=255) aTTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGc < 1:5648531/71‑1 (MQ=255) ttCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttgtttg < 1:664898/66‑1 (MQ=255) ttCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttgtttg < 1:1762238/66‑1 (MQ=255) ttCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCa < 1:2568366/71‑1 (MQ=255) ttCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCa > 1:5432269/1‑71 (MQ=255) tCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttgtttg < 1:5065524/65‑1 (MQ=255) tCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttgt > 1:3112648/1‑62 (MQ=255) tCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttgt > 1:728931/1‑62 (MQ=255) cTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttgttt > 1:4358461/1‑63 (MQ=255) cTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACt > 1:4523335/1‑71 (MQ=255) cACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACt < 1:463774/68‑1 (MQ=255) cACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGtt > 1:3220209/1‑71 (MQ=255) aCGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGttt < 1:1198125/71‑1 (MQ=255) cGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTa < 1:950548/71‑1 (MQ=255) cGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTa > 1:1146638/1‑71 (MQ=255) gcgcgcTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCAt > 1:2373590/1‑49 (MQ=255) gcgcgcTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTa > 1:2366779/1‑69 (MQ=255) cgcTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttg < 1:2168707/50‑1 (MQ=255) cgcTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttg < 1:2303913/50‑1 (MQ=255) cTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGAc > 1:4307277/1‑71 (MQ=255) tGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGAc > 1:5597332/1‑70 (MQ=255) gggCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGAcgg > 1:3454238/1‑71 (MQ=255) cATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttg < 1:4123589/40‑1 (MQ=255) cATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCa > 1:5668714/1‑49 (MQ=255) cATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCa > 1:1252992/1‑49 (MQ=255) cATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCa > 1:1570879/1‑49 (MQ=255) atgatgGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGa > 1:3274472/1‑71 (MQ=255) atgatgGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGa > 1:1404732/1‑71 (MQ=255) atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGa > 1:1562947/1‑41 (MQ=255) atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGa > 1:2387878/1‑41 (MQ=255) atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCAc > 1:3907023/1‑46 (MQ=255) atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGAcggcgg > 1:1608238/1‑64 (MQ=255) atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATg < 1:1738957/70‑1 (MQ=255) atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATg > 1:5324654/1‑70 (MQ=255) atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATg > 1:5325828/1‑70 (MQ=255) atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGc < 1:4526423/71‑1 (MQ=255) atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGc < 1:3084162/71‑1 (MQ=255) atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGc < 1:4406319/71‑1 (MQ=255) gatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGaa > 1:1079282/1‑39 (MQ=255) gatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGa > 1:4132793/1‑65 (MQ=255) gatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAgg < 1:1297316/71‑1 (MQ=255) atgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGTTGCAGGt < 1:33828/71‑1 (MQ=255) atgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAgg > 1:5211871/1‑70 (MQ=255) gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg < 1:2668341/71‑1 (MQ=255) gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg < 1:293709/71‑1 (MQ=255) gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg < 1:3166406/71‑1 (MQ=255) gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg < 1:806370/71‑1 (MQ=255) gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg < 1:5476324/71‑1 (MQ=255) gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgc < 1:4521844/70‑1 (MQ=255) gcttCTGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTAcccg > 1:3733837/3‑70 (MQ=255) gcttCTGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTACCCGt < 1:305927/69‑1 (MQ=255) gcttCTGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTACCCGt < 1:3008848/69‑1 (MQ=255) gcgtCTGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGt < 1:5138441/62‑1 (MQ=255) cGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg < 1:3595086/70‑1 (MQ=255) cGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGt < 1:4382262/71‑1 (MQ=255) cGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGt < 1:5282299/71‑1 (MQ=255) cGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGt < 1:3870259/71‑1 (MQ=255) gTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtg < 1:731110/71‑1 (MQ=255) gTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtg < 1:3025731/71‑1 (MQ=255) ttCTGGTATCACTTCGCCATTTTGTTTGAAGCACt > 1:182072/1‑35 (MQ=255) ttCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGttt < 1:4219324/39‑1 (MQ=255) ttCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTAt < 1:3386721/41‑1 (MQ=255) ttCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGc < 1:3724063/59‑1 (MQ=255) ttCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgc < 1:472205/71‑1 (MQ=255) cTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTAt > 1:656532/1‑39 (MQ=255) cTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGAcgg > 1:558162/1‑47 (MQ=255) cTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCa > 1:5628888/1‑58 (MQ=255) cTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgct < 1:330016/70‑1 (MQ=255) tGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGc < 1:3908714/56‑1 (MQ=255) tGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTACCCGttctgg > 1:3426748/1‑70 (MQ=255) tGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTACCCGttctgg > 1:4394724/1‑70 (MQ=255) aTCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcgcgc > 1:2687181/1‑71 (MQ=255) aTCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcg > 1:5136372/1‑68 (MQ=255) aTCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcg > 1:1395470/1‑68 (MQ=255) cACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAg > 1:5262176/1‑52 (MQ=255) cACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAg > 1:3783402/1‑52 (MQ=255) aCTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCtt > 1:627540/1‑70 (MQ=255) ttCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcg < 1:714393/54‑1 (MQ=255) tCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATg > 1:1132488/1‑71 (MQ=255) cGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTAt < 1:5523042/69‑1 (MQ=255) gCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgct > 1:3073553/1‑57 (MQ=255) gCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgct > 1:1514813/1‑57 (MQ=255) gCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgct > 1:2355952/1‑57 (MQ=255) ccATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTg < 1:5128654/71‑1 (MQ=255) cATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGc < 1:444402/71‑1 (MQ=255) | GGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGC > minE/386146‑386285 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |