| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 386,257 | A→G | 7.8% | A489A (GCA→GCG) | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 386,257 | 0 | A | G | 7.8% | 216.2 / 6.8 | 77 | A489A (GCA→GCG) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base A (30/41); new base G (3/3); total (33/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 | |||||||||||
GATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTCCTGGCCTGAAGCGGACC > minE/386189‑386326 | gatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAgg < 1:1297316/71‑1 (MQ=255) atgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGTTGCAGGt < 1:33828/71‑1 (MQ=255) atgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAgg > 1:5211871/1‑70 (MQ=255) gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg < 1:3166406/71‑1 (MQ=255) gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg < 1:293709/71‑1 (MQ=255) gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg < 1:5476324/71‑1 (MQ=255) gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg < 1:2668341/71‑1 (MQ=255) gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg < 1:806370/71‑1 (MQ=255) gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgc < 1:4521844/70‑1 (MQ=255) gcttCTGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTAcccg > 1:3733837/3‑70 (MQ=255) gcttCTGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTACCCGt < 1:305927/69‑1 (MQ=255) gcttCTGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTACCCGt < 1:3008848/69‑1 (MQ=255) gcgtCTGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGt < 1:5138441/62‑1 (MQ=255) cGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg < 1:3595086/70‑1 (MQ=255) cGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGt < 1:4382262/71‑1 (MQ=255) cGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGt < 1:5282299/71‑1 (MQ=255) cGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGt < 1:3870259/71‑1 (MQ=255) gTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtg < 1:731110/71‑1 (MQ=255) gTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtg < 1:3025731/71‑1 (MQ=255) ttCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgc < 1:472205/71‑1 (MQ=255) cTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCa > 1:5628888/1‑58 (MQ=255) cTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgct < 1:330016/70‑1 (MQ=255) tGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTACCCGttctgg > 1:3426748/1‑70 (MQ=255) tGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTACCCGttctgg > 1:4394724/1‑70 (MQ=255) aTCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcgcgc > 1:2687181/1‑71 (MQ=255) aTCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcg > 1:1395470/1‑68 (MQ=255) aTCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcg > 1:5136372/1‑68 (MQ=255) cACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAg > 1:5262176/1‑52 (MQ=255) cACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAg > 1:3783402/1‑52 (MQ=255) aCTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCtt > 1:627540/1‑70 (MQ=255) ttCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcg < 1:4238556/54‑1 (MQ=255) ttCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcg < 1:714393/54‑1 (MQ=255) tCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATg > 1:1132488/1‑71 (MQ=255) cGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTAt < 1:5523042/69‑1 (MQ=255) gCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgct > 1:3073553/1‑57 (MQ=255) gCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgct > 1:2355952/1‑57 (MQ=255) gCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgct > 1:1514813/1‑57 (MQ=255) ccATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTg < 1:5128654/71‑1 (MQ=255) cATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGc < 1:1934363/71‑1 (MQ=255) cATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGc < 1:444402/71‑1 (MQ=255) ttttGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAg < 1:3063722/71‑1 (MQ=255) tttgtttgAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAg > 1:1372550/1‑70 (MQ=255) ttgtttgAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTa > 1:4652966/1‑43 (MQ=255) ttgtttgAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgctgc > 1:5369582/1‑53 (MQ=255) ttgtttgAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTAt > 1:671389/1‑62 (MQ=255) gAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcgc < 1:4128685/49‑1 (MQ=255) gAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcgc < 1:4928386/49‑1 (MQ=255) gAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTa < 1:181016/55‑1 (MQ=255) gAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATctgc < 1:2314553/70‑1 (MQ=255) gAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGATGCGCGCTTTa < 1:927550/55‑1 (MQ=255) gAAGCACTGTTTATT‑TGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTa < 1:1710832/54‑1 (MQ=255) aaGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATctgctg < 1:4754531/71‑1 (MQ=255) aaGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATctgct < 1:1764216/70‑1 (MQ=255) tGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtg < 1:5249229/36‑1 (MQ=255) gTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgctg > 1:1157827/1‑38 (MQ=255) gTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgctg > 1:4421787/1‑38 (MQ=255) tttATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATctgc > 1:1737934/1‑61 (MQ=255) tttATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCgtg < 1:3732176/69‑1 (MQ=255) tATTCTGACGGCGGTGGATGCAGGTACGCGtgctgc > 1:3637979/1‑36 (MQ=255) tATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCgtggtg > 1:3963062/1‑70 (MQ=255) tATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGgtgt > 1:5088644/1‑71 (MQ=255) aTTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTAt > 1:1715966/1‑44 (MQ=255) cTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTcc > 1:2346000/1‑71 (MQ=255) aCGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGgtgt > 1:456495/1‑64 (MQ=255) aCGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGtc < 1:4305653/65‑1 (MQ=255) aCGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTcc < 1:262788/68‑1 (MQ=255) aCGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTCCTgg > 1:3769119/1‑71 (MQ=255) cggcggTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCgt < 1:3391405/58‑1 (MQ=255) cggcggTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTcc < 1:269545/67‑1 (MQ=255) ggcggTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGtct > 1:2776551/1‑64 (MQ=255) ggcggTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTCCTGGcc > 1:1775749/1‑71 (MQ=255) tGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTCCTGGCCTGaa < 1:4752994/70‑1 (MQ=255) gATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTCCTGGCCTGAAGCg > 1:3893224/1‑71 (MQ=255) gATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTCCTGGCCTGAAGCg < 1:2587683/71‑1 (MQ=255) gCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTCCTGGCCTGAAGCGGAc > 1:3580561/1‑71 (MQ=255) cAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTCCTGGCCTGAAGCGGAcc < 1:5419814/71‑1 (MQ=255) cAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTCCTGGCCTGAAGCGGAcc < 1:4426125/71‑1 (MQ=255) cAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTCCTGGCCTGAAGCGGAcc > 1:1694810/1‑71 (MQ=255) | GATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTCCTGGCCTGAAGCGGACC > minE/386189‑386326 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |