| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 387,114 | A→C | 7.4% | L12F (TTA→TTC) ‡ | ybdD → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 387,114 | 0 | A | C | 7.4% | 236.7 / 12.2 | 94 | L12F (TTA→TTC) ‡ | ybdD | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (52/35); new base C (5/2); total (57/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
TTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAC > minE/387050‑387183 | tttCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAg > 1:510136/1‑66 (MQ=255) tCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAgg < 1:436353/70‑1 (MQ=255) tCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAgg < 1:4838640/70‑1 (MQ=255) cAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAggcg > 1:5618781/1‑71 (MQ=255) aaCATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGgcggcg > 1:4153458/1‑70 (MQ=255) tGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCt < 1:2848884/71‑1 (MQ=255) aGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatg < 1:4917597/70‑1 (MQ=255) gCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTgatgat < 1:1957616/71‑1 (MQ=255) gAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCa > 1:215504/1‑50 (MQ=255) gAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga > 1:5376051/1‑67 (MQ=255) gAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg < 1:1631052/71‑1 (MQ=255) gAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg < 1:2177515/71‑1 (MQ=255) gAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg < 1:1790062/71‑1 (MQ=255) aGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtg < 1:1440691/62‑1 (MQ=255) ggCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTgatgat > 1:4865496/1‑66 (MQ=255) gCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAggcggc < 1:1628028/53‑1 (MQ=255) gCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTg < 1:1261203/67‑1 (MQ=255) cAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGc > 1:3990063/1‑71 (MQ=255) aaGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCt < 1:4857845/56‑1 (MQ=255) aGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTc < 1:2949382/42‑1 (MQ=255) aGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTc < 1:675230/42‑1 (MQ=255) aGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAg > 1:3806213/1‑53 (MQ=255) aGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAg > 1:1387564/1‑53 (MQ=255) aGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg < 1:172929/64‑1 (MQ=255) gATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAg < 1:3068182/38‑1 (MQ=255) aTGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAggcgg > 1:5330562/1‑46 (MQ=255) aTGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa > 1:1601391/1‑71 (MQ=255) aTGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa > 1:4886846/1‑71 (MQ=255) tGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt > 1:2949322/1‑71 (MQ=255) tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGa < 1:2905471/46‑1 (MQ=255) tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCt < 1:1866695/50‑1 (MQ=255) tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCt < 1:4828258/50‑1 (MQ=255) tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGt > 1:1954260/1‑60 (MQ=255) tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGt > 1:5027236/1‑60 (MQ=255) tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTa < 1:1122434/71‑1 (MQ=255) ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa > 1:2650542/1‑67 (MQ=255) ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc > 1:2445598/1‑71 (MQ=255) ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc > 1:1661989/1‑71 (MQ=255) tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc > 1:1729895/1‑70 (MQ=255) tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc > 1:2071149/1‑70 (MQ=255) tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc > 1:2450142/1‑70 (MQ=255) tCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTAt < 1:5027329/56‑1 (MQ=255) cACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACaa > 1:2493443/1‑70 (MQ=255) cACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc > 1:4476256/1‑71 (MQ=255) aCTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCt > 1:2551315/1‑42 (MQ=255) aCTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc > 1:305563/1‑70 (MQ=255) cTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc < 1:598127/69‑1 (MQ=255) ggCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga > 1:1795185/1‑44 (MQ=255) ggCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTa > 1:4115185/1‑50 (MQ=255) ggCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTAt > 1:596595/1‑51 (MQ=255) gCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTg < 1:27387/46‑1 (MQ=255) cAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGt > 1:169441/1‑70 (MQ=255) aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga > 1:2336443/1‑41 (MQ=255) aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg < 1:981279/45‑1 (MQ=255) aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg < 1:2746943/45‑1 (MQ=255) aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg < 1:2263016/45‑1 (MQ=255) aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt > 1:5572510/1‑55 (MQ=255) aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt > 1:5565807/1‑55 (MQ=255) aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTc > 1:4569649/1‑70 (MQ=255) aaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtg < 1:3806854/35‑1 (MQ=255) aGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGaa > 1:3932333/1‑70 (MQ=255) aGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc < 1:2296528/71‑1 (MQ=255) ccGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga > 1:4265816/1‑36 (MQ=255) ccGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACa > 1:4169278/1‑70 (MQ=255) cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa < 1:3742088/48‑1 (MQ=255) cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa < 1:4007465/48‑1 (MQ=255) cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa < 1:3234295/48‑1 (MQ=255) cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACaa < 1:4582039/57‑1 (MQ=255) aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata > 1:1368742/4‑71 (MQ=255) aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata > 1:2503899/4‑71 (MQ=255) aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata > 1:1375512/4‑71 (MQ=255) gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt > 1:1563660/1‑57 (MQ=255) gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt > 1:4746026/1‑57 (MQ=255) gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt > 1:3960817/1‑57 (MQ=255) gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt > 1:2710601/1‑57 (MQ=255) aaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg < 1:2994722/70‑1 (MQ=255) aaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg < 1:784947/70‑1 (MQ=255) aaaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt > 1:1798256/1‑46 (MQ=255) aaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTAc > 1:307246/1‑49 (MQ=255) aaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg > 1:2617577/1‑70 (MQ=255) aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg > 1:331928/1‑71 (MQ=255) aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg > 1:405943/1‑71 (MQ=255) aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg < 1:3826447/71‑1 (MQ=255) atatTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCggg > 1:5142009/1‑71 (MQ=255) atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt > 1:2606716/1‑70 (MQ=255) atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt > 1:3267710/1‑70 (MQ=255) atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt > 1:4408503/1‑70 (MQ=255) atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt > 1:1579984/1‑70 (MQ=255) atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt > 1:3907523/1‑70 (MQ=255) tttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa < 1:4862363/71‑1 (MQ=255) tttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa < 1:3716080/71‑1 (MQ=255) ttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaa > 1:2608251/1‑71 (MQ=255) ttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaa > 1:967722/1‑71 (MQ=255) tAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc > 1:4330098/1‑71 (MQ=255) aGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc < 1:342600/70‑1 (MQ=255) | TTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAC > minE/387050‑387183 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |