Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 387,144 G→T 11.4% M22I (ATG→ATT ybdD → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE387,1440GT11.4% 375.3 / 20.9 122M22I (ATG→ATTybdDconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (69/39);  new base T (8/6);  total (77/45)
Fisher's exact test for biased strand distribution p-value = 7.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 127 total aligned reads displayed.

CAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACG  >  minE/387075‑387211
                                                                     |                                                                   
cAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGc                                                                    >  1:3990063/1‑71 (MQ=255)
    aTGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                                >  1:1601391/1‑71 (MQ=255)
    aTGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                                >  1:4886846/1‑71 (MQ=255)
     tGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                               >  1:2949322/1‑71 (MQ=255)
       tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTa                                                             <  1:1122434/71‑1 (MQ=255)
        ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                            >  1:1661989/1‑71 (MQ=255)
        ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                            >  1:2445598/1‑71 (MQ=255)
         tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                            >  1:2071149/1‑70 (MQ=255)
         tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                            >  1:1729895/1‑70 (MQ=255)
              cACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACaa                                                       >  1:2493443/1‑70 (MQ=255)
              cACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                                                      >  1:4476256/1‑71 (MQ=255)
               aCTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                                                      >  1:305563/1‑70 (MQ=255)
                cTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                                                      <  1:598127/69‑1 (MQ=255)
                    cAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGt                                                 >  1:169441/1‑70 (MQ=255)
                     aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                               >  1:5572510/1‑55 (MQ=255)
                        aGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                            <  1:2296528/71‑1 (MQ=255)
                          ccGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACa                                           >  1:4169278/1‑70 (MQ=255)
                           cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                                <  1:3234295/48‑1 (MQ=255)
                           cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                                <  1:3742088/48‑1 (MQ=255)
                           cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACaa                                                       <  1:4582039/57‑1 (MQ=255)
                           aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata                                         >  1:1375512/4‑71 (MQ=255)
                           aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata                                         >  1:2503899/4‑71 (MQ=255)
                             gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                                     >  1:3960817/1‑57 (MQ=255)
                             gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                                     >  1:2710601/1‑57 (MQ=255)
                             gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                                     >  1:4746026/1‑57 (MQ=255)
                             gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                                     >  1:1563660/1‑57 (MQ=255)
                             aaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                       <  1:784947/70‑1 (MQ=255)
                              aaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTAc                                                            >  1:307246/1‑49 (MQ=255)
                              aaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                       >  1:2617577/1‑70 (MQ=255)
                               aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                                     >  1:405943/1‑71 (MQ=255)
                               aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                                     >  1:331928/1‑71 (MQ=255)
                               aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                                     <  1:3826447/71‑1 (MQ=255)
                                   atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                  >  1:1579984/1‑70 (MQ=255)
                                   atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                  >  1:3907523/1‑70 (MQ=255)
                                   atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                  >  1:4408503/1‑70 (MQ=255)
                                    tttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                                <  1:4862363/71‑1 (MQ=255)
                                     ttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaa                               >  1:2608251/1‑71 (MQ=255)
                                      tAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                              >  1:4330098/1‑71 (MQ=255)
                                       aGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                              <  1:342600/70‑1 (MQ=255)
                                             ggcggcGAAGCTGATGATTGGTATGCCTGATTACGACAACTa                                                    >  1:1023549/1‑42 (MQ=255)
                                               cggcgAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATgaa                                     >  1:5111859/1‑53 (MQ=255)
                                                ggcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACata                                         >  1:2670197/1‑50 (MQ=255)
                                                ggcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACata                                         >  1:4862206/1‑50 (MQ=255)
                                                 gcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAcc                             <  1:4683993/61‑1 (MQ=255)
                                                 gcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa                   <  1:4922145/71‑1 (MQ=255)
                                                 gcgAAGATGTTGATTGGCATTCCAGACTACGACAACTacg                                                  <  1:5546099/40‑3 (MQ=255)
                                                  cgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                                <  1:2599167/57‑1 (MQ=255)
                                                   gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                            <  1:1450240/44‑1 (MQ=255)
                                                   gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                            <  1:3675457/44‑1 (MQ=255)
                                                   gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                            <  1:2855109/44‑1 (MQ=255)
                                                   gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:5554143/1‑71 (MQ=255)
                                                    aaGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAAc                <  1:1930727/71‑1 (MQ=255)
                                                     aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGaa                                             >  1:4688802/1‑41 (MQ=255)
                                                     aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                                          >  1:3906435/1‑44 (MQ=255)
                                                     aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                                          >  1:4705646/1‑44 (MQ=255)
                                                     aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                                          >  1:3678828/1‑44 (MQ=255)
                                                      gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                    >  1:3400112/1‑49 (MQ=255)
                                                      gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                    >  1:141617/1‑49 (MQ=255)
                                                      gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                    >  1:4724351/1‑49 (MQ=255)
                                                      gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                    >  1:5383963/1‑49 (MQ=255)
                                                      gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                    >  1:4655295/1‑49 (MQ=255)
                                                       cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                              <  1:3094796/38‑1 (MQ=255)
                                                       cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                              <  1:3186493/38‑1 (MQ=255)
                                                       cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                              <  1:1829094/38‑1 (MQ=255)
                                                       cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATc                          >  1:3332601/1‑58 (MQ=255)
                                                       cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATc                    <  1:2425918/64‑1 (MQ=255)
                                                       cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa                   >  1:2193423/1‑65 (MQ=255)
                                                       cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc             <  1:2756149/71‑1 (MQ=255)
                                                       cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc             <  1:4705111/71‑1 (MQ=255)
                                                       cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc             <  1:263657/71‑1 (MQ=255)
                                                        tgatgaTTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:4794735/1‑66 (MQ=255)
                                                        tgatgaTTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa                   <  1:2149469/64‑1 (MQ=255)
                                                         gatgatTGGTATGCCTGATTACGACAACTATGTCGAAc                                            >  1:504197/1‑38 (MQ=255)
                                                         gatgatTGGTATGCCTGATTACGACAACTATGTCGAAc                                            >  1:1959478/1‑38 (MQ=255)
                                                         gatgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                                >  1:938213/1‑50 (MQ=255)
                                                         gatgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCg            <  1:2939755/70‑1 (MQ=255)
                                                         gatgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGa           >  1:4499753/1‑71 (MQ=255)
                                                          ttgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                       <  1:2616689/41‑1 (MQ=255)
                                                          ttgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                       <  1:4783303/41‑1 (MQ=255)
                                                          atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:790725/1‑64 (MQ=255)
                                                          atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:3227089/1‑64 (MQ=255)
                                                          atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGAt          <  1:2675114/71‑1 (MQ=255)
                                                           tgatTGGTATGCCTGATTACGACAACTATGTCGAACata                                         >  1:5366212/1‑39 (MQ=255)
                                                           tgatTGGTATGCCTGATTACGACAACTATGTCGAACata                                         >  1:1283650/1‑39 (MQ=255)
                                                            gatTGGTATGCCTGATTACGACAACTATGTCGAACa                                           >  1:3130891/1‑36 (MQ=255)
                                                            gatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:1087035/1‑62 (MQ=255)
                                                            gatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:906862/1‑62 (MQ=255)
                                                              ttGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGAcc      >  1:1485617/1‑71 (MQ=255)
                                                               tGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:4126632/1‑59 (MQ=255)
                                                                 gTATGCCTGATTACGACAACTATGTCGATCATATGCGGGTTAACCATCCCGATCAAACGCCGATGAcc      <  1:3956184/68‑1 (MQ=255)
                                                                  tATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACg  <  1:2161725/71‑1 (MQ=255)
                                                                   aTGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGAcc      <  1:4525697/66‑1 (MQ=255)
                                                                    tGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATc                    <  1:3262400/51‑1 (MQ=255)
                                                                    tGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATc                    <  1:2533314/51‑1 (MQ=255)
                                                                    tGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATg         <  1:962539/62‑1 (MQ=255)
                                                                     gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAcc                             <  1:477154/41‑1 (MQ=255)
                                                                     gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAcc                             <  1:3577061/41‑1 (MQ=255)
                                                                     gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCTTCCCGATCa                   <  1:5054868/51‑1 (MQ=255)
                                                                     gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATg         <  1:1930838/61‑1 (MQ=255)
                                                                     gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATg         <  1:1597639/61‑1 (MQ=255)
                                                                     |                                                                   
CAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACG  >  minE/387075‑387211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: