Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 387,147 T→A 10.9% P23P (CCT→CCA ybdD → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE387,1470TA10.9% 350.5 / 27.4 127P23P (CCT→CCAybdDconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (69/44);  new base A (8/6);  total (77/50)
Fisher's exact test for biased strand distribution p-value = 7.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

ATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACG  >  minE/387079‑387211
                                                                    |                                                                
aTGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                                >  1:4886846/1‑71 (MQ=255)
aTGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                                >  1:1601391/1‑71 (MQ=255)
 tGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                               >  1:2949322/1‑71 (MQ=255)
   tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTa                                                             <  1:1122434/71‑1 (MQ=255)
    ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                                >  1:2650542/1‑67 (MQ=255)
    ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                            >  1:1661989/1‑71 (MQ=255)
    ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                            >  1:2445598/1‑71 (MQ=255)
     tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                            >  1:2450142/1‑70 (MQ=255)
     tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                            >  1:1729895/1‑70 (MQ=255)
          cACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                                                      >  1:4476256/1‑71 (MQ=255)
           aCTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                                                      >  1:305563/1‑70 (MQ=255)
            cTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                                                      <  1:598127/69‑1 (MQ=255)
                 aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                               >  1:5565807/1‑55 (MQ=255)
                 aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTc                                                >  1:4569649/1‑70 (MQ=255)
                      ccGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACa                                           >  1:4169278/1‑70 (MQ=255)
                       cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                                <  1:3234295/48‑1 (MQ=255)
                       cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                                <  1:4007465/48‑1 (MQ=255)
                       cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACaa                                                       <  1:4582039/57‑1 (MQ=255)
                       aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata                                         >  1:1375512/4‑71 (MQ=255)
                       aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata                                         >  1:2503899/4‑71 (MQ=255)
                         gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                                     >  1:3960817/1‑57 (MQ=255)
                         gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                                     >  1:4746026/1‑57 (MQ=255)
                         gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                                     >  1:1563660/1‑57 (MQ=255)
                         aaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                       <  1:2994722/70‑1 (MQ=255)
                         aaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                       <  1:784947/70‑1 (MQ=255)
                          aaaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                               >  1:1798256/1‑46 (MQ=255)
                          aaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTAc                                                            >  1:307246/1‑49 (MQ=255)
                          aaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                       >  1:2617577/1‑70 (MQ=255)
                           aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                                     <  1:3826447/71‑1 (MQ=255)
                             atatTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCggg                                   >  1:5142009/1‑71 (MQ=255)
                               atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                  >  1:3267710/1‑70 (MQ=255)
                               atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                  >  1:2606716/1‑70 (MQ=255)
                               atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                  >  1:3907523/1‑70 (MQ=255)
                               atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                  >  1:4408503/1‑70 (MQ=255)
                               atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                  >  1:1579984/1‑70 (MQ=255)
                                tttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                                <  1:3716080/71‑1 (MQ=255)
                                 ttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaa                               >  1:2608251/1‑71 (MQ=255)
                                  tAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                              >  1:4330098/1‑71 (MQ=255)
                                   aGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                              <  1:342600/70‑1 (MQ=255)
                                         ggcggcGAAGCTGATGATTGGTATGCCTGATTACGACAACTa                                                    >  1:1023549/1‑42 (MQ=255)
                                           cggcgAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATgaa                                     >  1:5111859/1‑53 (MQ=255)
                                            ggcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACata                                         >  1:2670197/1‑50 (MQ=255)
                                            ggcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACata                                         >  1:4862206/1‑50 (MQ=255)
                                            ggcgAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATgaa                                     >  1:3133247/1‑52 (MQ=255)
                                             gcgAAGTTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaaac                             <  1:4942000/61‑3 (MQ=255)
                                             gcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAcc                             <  1:4683993/61‑1 (MQ=255)
                                             gcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa                   <  1:4922145/71‑1 (MQ=255)
                                             gcgAAGATGTTGATTGGCATTCCAGACTACGACAACTacg                                                  <  1:5546099/40‑3 (MQ=255)
                                               gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                            <  1:1450240/44‑1 (MQ=255)
                                               gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                            <  1:2855109/44‑1 (MQ=255)
                                               gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                            <  1:3675457/44‑1 (MQ=255)
                                               gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:5554143/1‑71 (MQ=255)
                                                 aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                                          >  1:4705646/1‑44 (MQ=255)
                                                 aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                                          >  1:3678828/1‑44 (MQ=255)
                                                 aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                                          >  1:3906435/1‑44 (MQ=255)
                                                  gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                    >  1:141617/1‑49 (MQ=255)
                                                  gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                    >  1:5383963/1‑49 (MQ=255)
                                                  gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                    >  1:4655295/1‑49 (MQ=255)
                                                  gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                    >  1:3400112/1‑49 (MQ=255)
                                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                              <  1:3402980/38‑1 (MQ=255)
                                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                              <  1:1829094/38‑1 (MQ=255)
                                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                              <  1:3186493/38‑1 (MQ=255)
                                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATc                          >  1:3332601/1‑58 (MQ=255)
                                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATc                    <  1:2425918/64‑1 (MQ=255)
                                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa                   >  1:2193423/1‑65 (MQ=255)
                                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc             <  1:4857755/71‑1 (MQ=255)
                                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc             <  1:4705111/71‑1 (MQ=255)
                                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc             <  1:2756149/71‑1 (MQ=255)
                                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGc              >  1:1988167/1‑70 (MQ=255)
                                                    tgatgaTTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:4794735/1‑66 (MQ=255)
                                                    tgatgaTTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:4691313/1‑66 (MQ=255)
                                                    tgatgaTTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa                   <  1:2149469/64‑1 (MQ=255)
                                                     gatgatTGGTATGCCTGATTACGACAACTATGTCGAAc                                            >  1:1959478/1‑38 (MQ=255)
                                                     gatgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                                >  1:938213/1‑50 (MQ=255)
                                                     gatgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGa           >  1:4499753/1‑71 (MQ=255)
                                                      ttgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                       <  1:4783303/41‑1 (MQ=255)
                                                      ttgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                       <  1:2616689/41‑1 (MQ=255)
                                                      ttgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                       <  1:2812804/41‑1 (MQ=255)
                                                      atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                              >  1:1637707/1‑51 (MQ=255)
                                                      atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                              >  1:2251007/1‑51 (MQ=255)
                                                      atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:790725/1‑64 (MQ=255)
                                                      atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGAt          <  1:2675114/71‑1 (MQ=255)
                                                       tgatTGGTATGCCTGATTACGACAACTATGTCGAACata                                         >  1:1283650/1‑39 (MQ=255)
                                                       tgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATgaa                                     >  1:1488582/1‑41 (MQ=255)
                                                        gatTGGTATGCCTGATTACGACAACTATGTCGAACa                                           >  1:3130891/1‑36 (MQ=255)
                                                        gatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:906862/1‑62 (MQ=255)
                                                        gatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:1087035/1‑62 (MQ=255)
                                                           tGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:4126632/1‑59 (MQ=255)
                                                             gTATGCCTGATTACGACAACTATGTCGATCATATGCGGGTTAACCATCCCGATCAAACGCCGATGAcc      <  1:3956184/68‑1 (MQ=255)
                                                             gTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGAcc      <  1:4742001/68‑1 (MQ=255)
                                                              tATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACg  <  1:2161725/71‑1 (MQ=255)
                                                                tGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATc                    <  1:3262400/51‑1 (MQ=255)
                                                                tGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATc                    <  1:2533314/51‑1 (MQ=255)
                                                                tGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATg         <  1:962539/62‑1 (MQ=255)
                                                                 gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCTTCCCGATCa                   <  1:5054868/51‑1 (MQ=255)
                                                                 gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATg         <  1:1930838/61‑1 (MQ=255)
                                                                  ccTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:4740736/1‑52 (MQ=255)
                                                                    tGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCg                       <  1:2534417/44‑1 (MQ=255)
                                                                    tGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATg         <  1:1671054/58‑1 (MQ=255)
                                                                    tGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCt     >  1:3957920/1‑62 (MQ=255)
                                                                    |                                                                
ATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACG  >  minE/387079‑387211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: