Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 387,162 T→C 9.8% Y28Y (TAT→TAC ybdD → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE387,1620TC 9.8% 329.0 / 20.2 122Y28Y (TAT→TACybdDconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (64/46);  new base C (7/5);  total (71/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

Only 100 of 126 total aligned reads displayed.

CAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTGAGCGG  >  minE/387095‑387231
                                                                   |                                                                     
cAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGt                                                                     >  1:169441/1‑70 (MQ=255)
 aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTc                                                                    >  1:4569649/1‑70 (MQ=255)
      ccGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACa                                                               >  1:4169278/1‑70 (MQ=255)
       aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata                                                             >  1:1375512/4‑71 (MQ=255)
         aaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                                           <  1:2994722/70‑1 (MQ=255)
         aaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                                           <  1:784947/70‑1 (MQ=255)
          aaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                                           >  1:2617577/1‑70 (MQ=255)
           aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                                                         >  1:405943/1‑71 (MQ=255)
           aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                                                         >  1:331928/1‑71 (MQ=255)
           aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                                                         <  1:3826447/71‑1 (MQ=255)
             atatTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCggg                                                       >  1:5142009/1‑71 (MQ=255)
               atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                                      >  1:1579984/1‑70 (MQ=255)
               atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                                      >  1:3907523/1‑70 (MQ=255)
               atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                                      >  1:3267710/1‑70 (MQ=255)
                tttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                                                    <  1:3716080/71‑1 (MQ=255)
                tttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                                                    <  1:4862363/71‑1 (MQ=255)
                 ttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaa                                                   >  1:2608251/1‑71 (MQ=255)
                  tAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                                                  >  1:4330098/1‑71 (MQ=255)
                   aGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                                                  <  1:342600/70‑1 (MQ=255)
                           cggcgAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATgaa                                                         >  1:5111859/1‑53 (MQ=255)
                            ggcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACata                                                             >  1:4862206/1‑50 (MQ=255)
                            ggcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACata                                                             >  1:2670197/1‑50 (MQ=255)
                             gcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAcc                                                 <  1:4683993/61‑1 (MQ=255)
                             gcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa                                       <  1:4922145/71‑1 (MQ=255)
                              cgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                                                    <  1:2599167/57‑1 (MQ=255)
                               gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                                                <  1:1450240/44‑1 (MQ=255)
                               gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                                                <  1:3675457/44‑1 (MQ=255)
                               gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                                     >  1:5554143/1‑71 (MQ=255)
                                aaGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAAc                                    <  1:1930727/71‑1 (MQ=255)
                                 aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGaa                                                                 >  1:4688802/1‑41 (MQ=255)
                                 aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                                                              >  1:3906435/1‑44 (MQ=255)
                                 aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                                                              >  1:4705646/1‑44 (MQ=255)
                                  gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                                        >  1:4655295/1‑49 (MQ=255)
                                  gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                                        >  1:5383963/1‑49 (MQ=255)
                                  gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                                        >  1:141617/1‑49 (MQ=255)
                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                                                  <  1:3402980/38‑1 (MQ=255)
                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                                                  <  1:3094796/38‑1 (MQ=255)
                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                                                  <  1:1829094/38‑1 (MQ=255)
                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                                                  <  1:3186493/38‑1 (MQ=255)
                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATc                                              >  1:3332601/1‑58 (MQ=255)
                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATc                                        <  1:2425918/64‑1 (MQ=255)
                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa                                       >  1:2193423/1‑65 (MQ=255)
                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc                                 <  1:263657/71‑1 (MQ=255)
                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc                                 <  1:4857755/71‑1 (MQ=255)
                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc                                 <  1:4705111/71‑1 (MQ=255)
                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc                                 <  1:2756149/71‑1 (MQ=255)
                                   cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGc                                  >  1:1988167/1‑70 (MQ=255)
                                     gatgatTGGTATGCCTGATTACGACAACTATGTCGAAc                                                                >  1:504197/1‑38 (MQ=255)
                                     gatgatTGGTATGCCTGATTACGACAACTATGTCGAAc                                                                >  1:1959478/1‑38 (MQ=255)
                                     gatgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                                                    >  1:938213/1‑50 (MQ=255)
                                     gatgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCg                                <  1:2939755/70‑1 (MQ=255)
                                     gatgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGa                               >  1:4499753/1‑71 (MQ=255)
                                      ttgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                                           <  1:2616689/41‑1 (MQ=255)
                                      ttgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                                           <  1:2812804/41‑1 (MQ=255)
                                      ttgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                                           <  1:4783303/41‑1 (MQ=255)
                                      atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                                                  >  1:1637707/1‑51 (MQ=255)
                                      atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                                                  >  1:2251007/1‑51 (MQ=255)
                                      atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                                     >  1:3227089/1‑64 (MQ=255)
                                      atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                                     >  1:790725/1‑64 (MQ=255)
                                      atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGAt                              <  1:2675114/71‑1 (MQ=255)
                                       tgatTGGTATGCCTGATTACGACAACTATGTCGAACata                                                             >  1:5366212/1‑39 (MQ=255)
                                       tgatTGGTATGCCTGATTACGACAACTATGTCGAACata                                                             >  1:1283650/1‑39 (MQ=255)
                                       tgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATgaa                                                         >  1:1488582/1‑41 (MQ=255)
                                        gatTGGTATGCCTGATTACGACAACTATGTCGAACa                                                               >  1:3130891/1‑36 (MQ=255)
                                        gatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                                     >  1:906862/1‑62 (MQ=255)
                                        gatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                                     >  1:1087035/1‑62 (MQ=255)
                                             gTATGCCTGATTACGACAACTATGTCGATCATATGCGGGTTAACCATCCCGATCAAACGCCGATGAcc                          <  1:3956184/68‑1 (MQ=255)
                                             gTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGAcc                          <  1:4742001/68‑1 (MQ=255)
                                              tATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACg                      <  1:2161725/71‑1 (MQ=255)
                                               aTGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGAcc                          <  1:4525697/66‑1 (MQ=255)
                                                tGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATc                                        <  1:2533314/51‑1 (MQ=255)
                                                tGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATg                             <  1:962539/62‑1 (MQ=255)
                                                tGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATg                             <  1:4388754/62‑1 (MQ=255)
                                                 gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAcc                                                 <  1:477154/41‑1 (MQ=255)
                                                 gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAcc                                                 <  1:3577061/41‑1 (MQ=255)
                                                 gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCTTCCCGATCa                                       <  1:5054868/51‑1 (MQ=255)
                                                 gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATg                             <  1:1597639/61‑1 (MQ=255)
                                                  ccTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                                     >  1:4740736/1‑52 (MQ=255)
                                                    tGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCg                                           <  1:2534417/44‑1 (MQ=255)
                                                    tGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCt                         >  1:3957920/1‑62 (MQ=255)
                                                      aTTACGACAACTATGTCGAACATATGCGGGTTAACCATccc                                            >  1:214245/1‑41 (MQ=255)
                                                      aTTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGAt                                         >  1:2376903/1‑44 (MQ=255)
                                                      aTTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc                                 <  1:600208/52‑1 (MQ=255)
                                                      aTTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGttt              >  1:2478839/1‑71 (MQ=255)
                                                      aTTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGt                <  1:3980287/69‑1 (MQ=255)
                                                       ttACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGtttt             <  1:2078956/71‑1 (MQ=255)
                                                       ttACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGtttt             >  1:3234825/1‑71 (MQ=255)
                                                        tACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAAc                                    >  1:221348/1‑47 (MQ=255)
                                                        tACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGttttt            >  1:1891733/1‑71 (MQ=255)
                                                        tACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGttttt            >  1:3238392/1‑71 (MQ=255)
                                                        tACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGttttt            >  1:4407612/1‑71 (MQ=255)
                                                              aaCTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGt        >  1:5380489/1‑69 (MQ=255)
                                                                cTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGAt                              >  1:4705012/1‑45 (MQ=255)
                                                                cTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAagag                 >  1:4536610/1‑58 (MQ=255)
                                                                cTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAagag                 >  1:5072475/1‑58 (MQ=255)
                                                                cTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTg       <  1:2508994/68‑1 (MQ=255)
                                                                  aTGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTGAGCgg  >  1:1242304/1‑71 (MQ=255)
                                                                   tGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGttt              >  1:465094/1‑58 (MQ=255)
                                                                   tGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGttt              >  1:1299555/1‑58 (MQ=255)
                                                                   tGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTGAGCgg  <  1:1479597/70‑1 (MQ=255)
                                                                   |                                                                     
CAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTGAGCGG  >  minE/387095‑387231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: