Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 387,168 A→G 10.0% E30E (GAA→GAG ybdD → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE387,1680AG10.0% 321.1 / 20.3 121E30E (GAA→GAGybdDconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (66/42);  new base G (7/5);  total (73/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

Only 100 of 123 total aligned reads displayed.

AGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTGAGCGG  >  minE/387099‑387231
                                                                     |                                                               
aGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                                                <  1:2296528/71‑1 (MQ=255)
  ccGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACa                                                               >  1:4169278/1‑70 (MQ=255)
   aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata                                                             >  1:1368742/4‑71 (MQ=255)
   aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata                                                             >  1:2503899/4‑71 (MQ=255)
     aaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                                           <  1:2994722/70‑1 (MQ=255)
     aaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                                           <  1:784947/70‑1 (MQ=255)
      aaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                                           >  1:2617577/1‑70 (MQ=255)
       aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                                                         <  1:3826447/71‑1 (MQ=255)
       aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                                                         >  1:405943/1‑71 (MQ=255)
         atatTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCggg                                                       >  1:5142009/1‑71 (MQ=255)
           atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                                      >  1:4408503/1‑70 (MQ=255)
           atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                                      >  1:3907523/1‑70 (MQ=255)
           atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                                      >  1:3267710/1‑70 (MQ=255)
           atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                                      >  1:2606716/1‑70 (MQ=255)
           atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                                      >  1:1579984/1‑70 (MQ=255)
            tttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                                                    <  1:3716080/71‑1 (MQ=255)
            tttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                                                    <  1:4862363/71‑1 (MQ=255)
             ttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaa                                                   >  1:2608251/1‑71 (MQ=255)
              tAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                                                  >  1:4330098/1‑71 (MQ=255)
               aGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                                                  <  1:342600/70‑1 (MQ=255)
                       cggcgAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATgaa                                                         >  1:5111859/1‑53 (MQ=255)
                        ggcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACata                                                             >  1:4862206/1‑50 (MQ=255)
                        ggcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACata                                                             >  1:2670197/1‑50 (MQ=255)
                        ggcgAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATgaa                                                         >  1:3133247/1‑52 (MQ=255)
                         gcgAAGTTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaaac                                                 <  1:4942000/61‑3 (MQ=255)
                         gcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAcc                                                 <  1:4683993/61‑1 (MQ=255)
                           gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                                                <  1:3675457/44‑1 (MQ=255)
                           gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                                                <  1:1450240/44‑1 (MQ=255)
                           gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                                     >  1:5554143/1‑71 (MQ=255)
                             aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGaa                                                                 >  1:4688802/1‑41 (MQ=255)
                             aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                                                              >  1:3906435/1‑44 (MQ=255)
                             aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                                                              >  1:4705646/1‑44 (MQ=255)
                              gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                                        >  1:829640/1‑49 (MQ=255)
                              gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                                        >  1:3400112/1‑49 (MQ=255)
                              gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                                        >  1:141617/1‑49 (MQ=255)
                              gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                                        >  1:4724351/1‑49 (MQ=255)
                               cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATc                                              >  1:3332601/1‑58 (MQ=255)
                               cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATc                                        <  1:2425918/64‑1 (MQ=255)
                               cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa                                       >  1:2193423/1‑65 (MQ=255)
                               cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc                                 <  1:2756149/71‑1 (MQ=255)
                               cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc                                 <  1:4857755/71‑1 (MQ=255)
                               cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGc                                  >  1:1988167/1‑70 (MQ=255)
                                tgatgaTTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                                     >  1:4794735/1‑66 (MQ=255)
                                tgatgaTTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                                     >  1:4691313/1‑66 (MQ=255)
                                tgatgaTTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa                                       <  1:2149469/64‑1 (MQ=255)
                                 gatgatTGGTATGCCTGATTACGACAACTATGTCGAAc                                                                >  1:504197/1‑38 (MQ=255)
                                 gatgatTGGTATGCCTGATTACGACAACTATGTCGAAc                                                                >  1:1959478/1‑38 (MQ=255)
                                 gatgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                                                    >  1:938213/1‑50 (MQ=255)
                                 gatgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCg                                <  1:2939755/70‑1 (MQ=255)
                                  ttgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                                           <  1:2616689/41‑1 (MQ=255)
                                  ttgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                                           <  1:2812804/41‑1 (MQ=255)
                                  atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                                                  >  1:2251007/1‑51 (MQ=255)
                                  atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                                                  >  1:1637707/1‑51 (MQ=255)
                                  atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGAt                              <  1:2675114/71‑1 (MQ=255)
                                   tgatTGGTATGCCTGATTACGACAACTATGTCGAACata                                                             >  1:1283650/1‑39 (MQ=255)
                                   tgatTGGTATGCCTGATTACGACAACTATGTCGAACata                                                             >  1:5366212/1‑39 (MQ=255)
                                    gatTGGTATGCCTGATTACGACAACTATGTCGAACa                                                               >  1:3130891/1‑36 (MQ=255)
                                    gatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                                     >  1:1087035/1‑62 (MQ=255)
                                    gatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                                     >  1:906862/1‑62 (MQ=255)
                                      ttGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGAcc                          >  1:1485617/1‑71 (MQ=255)
                                         gTATGCCTGATTACGACAACTATGTCGATCATATGCGGGTTAACCATCCCGATCAAACGCCGATGAcc                          <  1:3956184/68‑1 (MQ=255)
                                         gTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGAcc                          <  1:4742001/68‑1 (MQ=255)
                                          tATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACg                      <  1:2161725/71‑1 (MQ=255)
                                           aTGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGAcc                          <  1:4525697/66‑1 (MQ=255)
                                            tGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATc                                        <  1:3262400/51‑1 (MQ=255)
                                            tGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATc                                        <  1:2533314/51‑1 (MQ=255)
                                            tGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATg                             <  1:962539/62‑1 (MQ=255)
                                            tGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATg                             <  1:4388754/62‑1 (MQ=255)
                                             gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAcc                                                 <  1:3577061/41‑1 (MQ=255)
                                             gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAcc                                                 <  1:477154/41‑1 (MQ=255)
                                             gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCTTCCCGATCa                                       <  1:5054868/51‑1 (MQ=255)
                                             gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATg                             <  1:1597639/61‑1 (MQ=255)
                                             gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATg                             <  1:1930838/61‑1 (MQ=255)
                                              ccTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                                     >  1:4740736/1‑52 (MQ=255)
                                                tGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCg                                           <  1:2534417/44‑1 (MQ=255)
                                                tGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATg                             <  1:1671054/58‑1 (MQ=255)
                                                tGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCt                         >  1:3957920/1‑62 (MQ=255)
                                                  aTTACGACAACTATGTCGAACATATGCGGGTTAACCATccc                                            >  1:214245/1‑41 (MQ=255)
                                                  aTTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGAt                                         >  1:2376903/1‑44 (MQ=255)
                                                  aTTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc                                 <  1:600208/52‑1 (MQ=255)
                                                  aTTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGttt              >  1:2478839/1‑71 (MQ=255)
                                                  aTTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGt                <  1:3980287/69‑1 (MQ=255)
                                                   ttACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGtttt             >  1:3234825/1‑71 (MQ=255)
                                                   ttACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGtttt             <  1:2078956/71‑1 (MQ=255)
                                                    tACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGttttt            >  1:3238392/1‑71 (MQ=255)
                                                    tACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGttttt            >  1:1891733/1‑71 (MQ=255)
                                                    tACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGttttt            >  1:4407612/1‑71 (MQ=255)
                                                          aaCTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGa                               <  1:4616788/46‑1 (MQ=255)
                                                          aaCTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGt        >  1:5380489/1‑69 (MQ=255)
                                                            cTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc                                 <  1:5124250/42‑1 (MQ=255)
                                                            cTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGAt                              >  1:4705012/1‑45 (MQ=255)
                                                            cTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAagag                 >  1:5072475/1‑58 (MQ=255)
                                                            cTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAagag                 >  1:4536610/1‑58 (MQ=255)
                                                            cTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTg       <  1:2508994/68‑1 (MQ=255)
                                                              aTGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTGAGCgg  >  1:2651467/1‑71 (MQ=255)
                                                              aTGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTGAGCgg  >  1:1242304/1‑71 (MQ=255)
                                                               tGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGttt              >  1:465094/1‑58 (MQ=255)
                                                               tGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGttt              >  1:1299555/1‑58 (MQ=255)
                                                               tGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTGAGCgg  >  1:5423007/1‑70 (MQ=255)
                                                               tGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTGAGCgg  <  1:1479597/70‑1 (MQ=255)
                                                                     |                                                               
AGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTTTCCGTGAGCGG  >  minE/387099‑387231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: