| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 453,891 | G→C | 6.9% | A398A (GCC→GCG) | ybgH ← | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 453,891 | 0 | G | C | 6.9% | 212.8 / 5.2 | 73 | A398A (GCC→GCG) | ybgH | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base G (38/29); new base C (3/2); total (41/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
AAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAA > minE/453822‑453960 | aaGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATgg > 1:1560343/1‑71 (MQ=255)aaGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATgg < 1:1867766/71‑1 (MQ=255) tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCggg > 1:3191217/1‑71 (MQ=255) tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCggg < 1:457871/71‑1 (MQ=255) tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCggg < 1:1996475/71‑1 (MQ=255) tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCggg < 1:3208593/71‑1 (MQ=255) tAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTc > 1:1880966/1‑71 (MQ=255) tAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTc > 1:265231/1‑71 (MQ=255) tGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGAt > 1:4846603/1‑70 (MQ=255) tGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa < 1:1441290/71‑1 (MQ=255) tGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa < 1:2435023/71‑1 (MQ=255) gCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa < 1:2931615/70‑1 (MQ=255) ccGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa < 1:4444835/71‑1 (MQ=255) gTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAg > 1:4184714/1‑71 (MQ=255) aaTACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGtt < 1:810175/70‑1 (MQ=255) aaTACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGtt < 1:1686551/70‑1 (MQ=255) cGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGc > 1:842821/1‑70 (MQ=255) ccGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa < 1:5291374/71‑1 (MQ=255) cacaCCGGGGATTTCAATGCGCGTAATTTGCGACATGGCa < 1:3417779/40‑1 (MQ=255) cacaCCGGGGATTTCAATGCGCGTAATTTGCGACATGGCa < 1:833701/40‑1 (MQ=255) cacaCCGGGGATTTCAATGCGCGTAATTTGCGACATGGCa < 1:3103625/40‑1 (MQ=255) cacaCCGGGGATTTCAATGCGCGTAATTAGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGccc > 1:2239834/1‑70 (MQ=255) acacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCAt > 1:4220582/1‑71 (MQ=255) cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc < 1:5337443/53‑1 (MQ=255) cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc < 1:1644636/53‑1 (MQ=255) cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc < 1:158017/53‑1 (MQ=255) cacCGGAGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAACGCCCAt > 1:5900/1‑70 (MQ=255) acCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAAc > 1:3193616/1‑39 (MQ=255) acCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAAc > 1:3661368/1‑39 (MQ=255) ccGGGGATTTCAATGCGCGTAATTTGCGACATGGCAAc > 1:3610001/1‑38 (MQ=255) ccGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa > 1:2512915/1‑48 (MQ=255) cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc > 1:2404714/1‑71 (MQ=255) cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc > 1:2310920/1‑71 (MQ=255) cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc > 1:4167049/1‑71 (MQ=255) cGGGGATTTCAATGCGCCTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa < 1:345052/61‑1 (MQ=255) ggggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCg > 1:11578/1‑71 (MQ=255) gggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa > 1:3139831/1‑47 (MQ=255) gggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa > 1:5340545/1‑47 (MQ=255) gATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAg > 1:4070724/1‑58 (MQ=255) aTTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGcc < 1:957240/69‑1 (MQ=255) aTTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAg > 1:4179011/1‑71 (MQ=255) tttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAgg > 1:2737780/1‑71 (MQ=255) tttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAgg > 1:4826648/1‑71 (MQ=255) ttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCAt < 1:3018891/60‑1 (MQ=255) cAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa > 1:1095301/1‑40 (MQ=255) tGCGCGTAATTTGCGACATTGCAACCGGGTCGATa < 1:5176365/35‑1 (MQ=255) tGCGCGTAATTTGCGACATGGCAACCGGGTCGATa < 1:3526013/35‑1 (MQ=255) tGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa > 1:196897/1‑70 (MQ=255) tGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAAt < 1:492960/71‑1 (MQ=255) gcgcGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCa > 1:3699689/1‑62 (MQ=255) cgcgTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGccc > 1:3348645/1‑51 (MQ=255) gtgaTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATATCAt > 1:4427525/4‑71 (MQ=255) tAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCt < 1:3640485/62‑1 (MQ=255) tAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCt < 1:3508119/62‑1 (MQ=255) aaTTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa > 1:3765145/1‑42 (MQ=255) aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCa > 1:5094101/1‑46 (MQ=255) aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCa > 1:3732216/1‑46 (MQ=255) aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATAtcatcac < 1:4273078/71‑2 (MQ=255) aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATAtcatcac < 1:2031314/71‑2 (MQ=255) aTTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAAt > 1:2499133/1‑63 (MQ=255) tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGc < 1:4969394/37‑1 (MQ=255) tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGc < 1:3443255/37‑1 (MQ=255) tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa > 1:4457961/1‑61 (MQ=255) tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa > 1:937381/1‑61 (MQ=255) tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa > 1:315219/1‑61 (MQ=255) tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa > 1:2579874/1‑61 (MQ=255) tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa > 1:655023/1‑61 (MQ=255) tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCa > 1:93137/1‑46 (MQ=255) tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGTCCTAATACCATCAGTgg > 1:199680/1‑71 (MQ=255) tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTgg > 1:542266/1‑71 (MQ=255) gACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCag < 1:63939/71‑1 (MQ=255) aCATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCa > 1:2817230/1‑69 (MQ=255) tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa < 1:1431740/71‑1 (MQ=255) tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa < 1:4152717/71‑1 (MQ=255) tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa < 1:496719/71‑1 (MQ=255) | AAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAA > minE/453822‑453960 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |