Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	453,894	A→C	6.9%	V397V (GTT→GTG)	ybgH ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	453,894	0	A	C	6.9%	179.3 / 7.1	72	V397V (GTT→GTG)	ybgH	predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (39/28);  new base C (3/2);  total (42/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01

TGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAG  >  minE/453830‑453961                                                                 |                                                                    tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCggg                                                               <  1:3208593/71‑1 (MQ=255) tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCggg                                                               <  1:457871/71‑1 (MQ=255) tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCggg                                                               >  1:3191217/1‑71 (MQ=255) tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCggg                                                               <  1:1996475/71‑1 (MQ=255)   tAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTc                                                             >  1:265231/1‑71 (MQ=255)   tAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTc                                                             >  1:1880966/1‑71 (MQ=255)       tGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGAt                                                          >  1:4846603/1‑70 (MQ=255)       tGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                         <  1:1441290/71‑1 (MQ=255)       tGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                         <  1:2435023/71‑1 (MQ=255)        gCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                         <  1:2931615/70‑1 (MQ=255)         ccGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa                                                       <  1:4444835/71‑1 (MQ=255)            gTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAg                                                    >  1:4184714/1‑71 (MQ=255)               aaTACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGtt                                                  <  1:1686551/70‑1 (MQ=255)               aaTACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGtt                                                  <  1:810175/70‑1 (MQ=255)                   cGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                              >  1:842821/1‑70 (MQ=255)                     ccGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                           <  1:5291374/71‑1 (MQ=255)                          cacaCCGGGGATTTCAATGCGCGTAATTTGCGACATGGCa                                                                     <  1:3103625/40‑1 (MQ=255)                          cacaCCGGGGATTTCAATGCGCGTAATTTGCGACATGGCa                                                                     <  1:3417779/40‑1 (MQ=255)                          cacaCCGGGGATTTCAATGCGCGTAATTTGCGACATGGCa                                                                     <  1:833701/40‑1 (MQ=255)                          cacaCCGGGGATTTCAATGCGCGTAATTAGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGccc                                       >  1:2239834/1‑70 (MQ=255)                           acacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCAt                                     >  1:4220582/1‑71 (MQ=255)                            cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc                                                      <  1:5337443/53‑1 (MQ=255)                            cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc                                                      <  1:1644636/53‑1 (MQ=255)                            cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc                                                      <  1:158017/53‑1 (MQ=255)                            cacCGGAGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAACGCCCAt                                     >  1:5900/1‑70 (MQ=255)                             acCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAAc                                                                   >  1:3661368/1‑39 (MQ=255)                             acCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAAc                                                                   >  1:3193616/1‑39 (MQ=255)                              ccGGGGATTTCAATGCGCGTAATTTGCGACATGGCAAc                                                                   >  1:3610001/1‑38 (MQ=255)                              ccGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                         >  1:2512915/1‑48 (MQ=255)                               cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                 >  1:2404714/1‑71 (MQ=255)                               cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                 >  1:2310920/1‑71 (MQ=255)                               cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                 >  1:4167049/1‑71 (MQ=255)                               cGGGGATTTCAATGCGCCTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                           <  1:345052/61‑1 (MQ=255)                                ggggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCg                                >  1:11578/1‑71 (MQ=255)                                 gggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa                                                       >  1:5340545/1‑47 (MQ=255)                                 gggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa                                                       >  1:3139831/1‑47 (MQ=255)                                   gATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAg                                          >  1:4070724/1‑58 (MQ=255)                                    aTTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGcc                              <  1:957240/69‑1 (MQ=255)                                    aTTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAg                            >  1:4179011/1‑71 (MQ=255)                                     tttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAgg                           >  1:4826648/1‑71 (MQ=255)                                     tttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAgg                           >  1:2737780/1‑71 (MQ=255)                                      ttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCAt                                     <  1:3018891/60‑1 (MQ=255)                                        cAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa                                                       >  1:1095301/1‑40 (MQ=255)                                           tGCGCGTAATTTGCGACATTGCAACCGGGTCGATa                                                         <  1:5176365/35‑1 (MQ=255)                                           tGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                         <  1:3526013/35‑1 (MQ=255)                                           tGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                      >  1:196897/1‑70 (MQ=255)                                           tGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAAt                     <  1:492960/71‑1 (MQ=255)                                            gcgcGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCa                             >  1:3699689/1‑62 (MQ=255)                                             cgcgTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGccc                                       >  1:3348645/1‑51 (MQ=255)                                                gtgaTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATATCAt                >  1:4427525/4‑71 (MQ=255)                                                 tAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCt                        <  1:3508119/62‑1 (MQ=255)                                                 tAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCt                        <  1:3640485/62‑1 (MQ=255)                                                  aaTTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                           >  1:3765145/1‑42 (MQ=255)                                                   aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCa                                      >  1:3732216/1‑46 (MQ=255)                                                   aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCa                                      >  1:5094101/1‑46 (MQ=255)                                                   aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATAtcatcac             <  1:4273078/71‑2 (MQ=255)                                                   aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATAtcatcac             <  1:2031314/71‑2 (MQ=255)                                                   aTTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAAt                     >  1:2499133/1‑63 (MQ=255)                                                    tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                              <  1:4969394/37‑1 (MQ=255)                                                    tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                              <  1:3443255/37‑1 (MQ=255)                                                    tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                      >  1:2579874/1‑61 (MQ=255)                                                    tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                      >  1:937381/1‑61 (MQ=255)                                                    tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                      >  1:4457961/1‑61 (MQ=255)                                                    tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                      >  1:315219/1‑61 (MQ=255)                                                    tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                      >  1:655023/1‑61 (MQ=255)                                                      tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCa                                   >  1:93137/1‑46 (MQ=255)                                                      tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGTCCTAATACCATCAGTgg          >  1:199680/1‑71 (MQ=255)                                                      tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTgg          >  1:542266/1‑71 (MQ=255)                                                         gACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCag       <  1:63939/71‑1 (MQ=255)                                                          aCATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCa        >  1:2817230/1‑69 (MQ=255)                                                             tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa   <  1:496719/71‑1 (MQ=255)                                                             tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa   <  1:1431740/71‑1 (MQ=255)                                                             tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa   <  1:4152717/71‑1 (MQ=255)                                                               gCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaag  <  1:2664782/70‑1 (MQ=255)                                                               gCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa   >  1:2964123/1‑69 (MQ=255)                                                                 aaCCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCagag     <  1:970742/65‑1 (MQ=255)                                                                 |                                                                    TGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAG  >  minE/453830‑453961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑