Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	453,903	G→A	6.8%	I394I (ATC→ATT)	ybgH ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	453,903	0	G	A	6.8%	214.2 / 4.3	73	I394I (ATC→ATT)	ybgH	predicted transporter
Reads supporting (aligned to +/- strand):  ref base G (41/27);  new base A (3/2);  total (44/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGAC  >  minE/453836‑453967                                                                    |                                                                 tGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGAt                                                                >  1:4846603/1‑70 (MQ=255) tGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                               <  1:1441290/71‑1 (MQ=255) tGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                               <  1:2435023/71‑1 (MQ=255)  gCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                               <  1:2931615/70‑1 (MQ=255)   ccGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa                                                             <  1:4444835/71‑1 (MQ=255)      gTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAg                                                          >  1:4184714/1‑71 (MQ=255)         aaTACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGtt                                                        <  1:1686551/70‑1 (MQ=255)         aaTACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGtt                                                        <  1:810175/70‑1 (MQ=255)             cGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                                    >  1:842821/1‑70 (MQ=255)               ccGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                                 <  1:5291374/71‑1 (MQ=255)                    cacaCCGGGGATTTCAATGCGCGTAATTAGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGccc                                             >  1:2239834/1‑70 (MQ=255)                     acacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCAt                                           >  1:4220582/1‑71 (MQ=255)                      cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc                                                            <  1:5337443/53‑1 (MQ=255)                      cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc                                                            <  1:1644636/53‑1 (MQ=255)                      cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc                                                            <  1:158017/53‑1 (MQ=255)                      cacCGGAGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAACGCCCAt                                           >  1:5900/1‑70 (MQ=255)                        ccGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                               >  1:2512915/1‑48 (MQ=255)                         cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                       >  1:2310920/1‑71 (MQ=255)                         cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                       >  1:2404714/1‑71 (MQ=255)                         cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                       >  1:4167049/1‑71 (MQ=255)                         cGGGGATTTCAATGCGCCTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                                 <  1:345052/61‑1 (MQ=255)                          ggggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCg                                      >  1:11578/1‑71 (MQ=255)                           gggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa                                                             >  1:3139831/1‑47 (MQ=255)                           gggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa                                                             >  1:5340545/1‑47 (MQ=255)                             gATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAg                                                >  1:4070724/1‑58 (MQ=255)                              aTTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGcc                                    <  1:957240/69‑1 (MQ=255)                              aTTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAg                                  >  1:4179011/1‑71 (MQ=255)                               tttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAgg                                 >  1:2737780/1‑71 (MQ=255)                               tttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAgg                                 >  1:4826648/1‑71 (MQ=255)                                ttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCAt                                           <  1:3018891/60‑1 (MQ=255)                                  cAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa                                                             >  1:1095301/1‑40 (MQ=255)                                     tGCGCGTAATTTGCGACATTGCAACCGGGTCGATa                                                               <  1:5176365/35‑1 (MQ=255)                                     tGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                               <  1:3526013/35‑1 (MQ=255)                                     tGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                            >  1:196897/1‑70 (MQ=255)                                     tGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAAt                           <  1:492960/71‑1 (MQ=255)                                      gcgcGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCa                                   >  1:3699689/1‑62 (MQ=255)                                       cgcgTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGccc                                             >  1:3348645/1‑51 (MQ=255)                                          gtgaTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATATCAt                      >  1:4427525/4‑71 (MQ=255)                                           tAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCt                              <  1:3640485/62‑1 (MQ=255)                                           tAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCt                              <  1:3508119/62‑1 (MQ=255)                                            aaTTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                                 >  1:3765145/1‑42 (MQ=255)                                             aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCa                                            >  1:3732216/1‑46 (MQ=255)                                             aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCa                                            >  1:5094101/1‑46 (MQ=255)                                             aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATAtcatcac                   <  1:4273078/71‑2 (MQ=255)                                             aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATAtcatcac                   <  1:2031314/71‑2 (MQ=255)                                             aTTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAAt                           >  1:2499133/1‑63 (MQ=255)                                              tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                                    <  1:3443255/37‑1 (MQ=255)                                              tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                                    <  1:4969394/37‑1 (MQ=255)                                              tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                            >  1:315219/1‑61 (MQ=255)                                              tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                            >  1:2579874/1‑61 (MQ=255)                                              tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                            >  1:937381/1‑61 (MQ=255)                                              tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                            >  1:4457961/1‑61 (MQ=255)                                              tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                            >  1:655023/1‑61 (MQ=255)                                                tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCa                                         >  1:93137/1‑46 (MQ=255)                                                tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGTCCTAATACCATCAGTgg                >  1:199680/1‑71 (MQ=255)                                                tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTgg                >  1:542266/1‑71 (MQ=255)                                                   gACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCag             <  1:63939/71‑1 (MQ=255)                                                    aCATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCa              >  1:2817230/1‑69 (MQ=255)                                                       tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa         <  1:4152717/71‑1 (MQ=255)                                                       tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa         <  1:1431740/71‑1 (MQ=255)                                                       tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa         <  1:496719/71‑1 (MQ=255)                                                         gCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaag        <  1:2664782/70‑1 (MQ=255)                                                         gCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa         >  1:2964123/1‑69 (MQ=255)                                                           aaCCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCagag           <  1:970742/65‑1 (MQ=255)                                                             ccGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGa   <  1:3441930/71‑1 (MQ=255)                                                              cGGTTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGAc  >  1:3428610/1‑71 (MQ=255)                                                              cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATAcc                        >  1:4291826/1‑49 (MQ=255)                                                              cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaaga       >  1:4803228/1‑66 (MQ=255)                                                              cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaaga       >  1:3224927/1‑66 (MQ=255)                                                              cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaaga       >  1:3739576/1‑66 (MQ=255)                                                              cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGAc  >  1:2630935/1‑71 (MQ=255)                                                              cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGAc  >  1:1983394/1‑71 (MQ=255)                                                                ggTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCa                    >  1:4381831/1‑51 (MQ=255)                                                                    |                                                                 TGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGAC  >  minE/453836‑453967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑