Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	453,909	C→G	8.1%	L392L (CTG→CTC)	ybgH ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	453,909	0	C	G	8.1%	181.1 / 3.6	61	L392L (CTG→CTC)	ybgH	predicted transporter
Reads supporting (aligned to +/- strand):  ref base C (37/19);  new base G (3/2);  total (40/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTG  >  minE/453841‑453976                                                                     |                                                                    gTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAg                                                                   >  1:4184714/1‑71 (MQ=255)    aaTACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGtt                                                                 <  1:1686551/70‑1 (MQ=255)    aaTACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGtt                                                                 <  1:810175/70‑1 (MQ=255)        cGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                                             >  1:842821/1‑70 (MQ=255)          ccGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                                          <  1:5291374/71‑1 (MQ=255)               cacaCCGGGGATTTCAATGCGCGTAATTAGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGccc                                                      >  1:2239834/1‑70 (MQ=255)                acacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCAt                                                    >  1:4220582/1‑71 (MQ=255)                 cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc                                                                     <  1:158017/53‑1 (MQ=255)                 cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc                                                                     <  1:1644636/53‑1 (MQ=255)                 cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc                                                                     <  1:5337443/53‑1 (MQ=255)                 cacCGGAGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAACGCCCAt                                                    >  1:5900/1‑70 (MQ=255)                    cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                                >  1:2310920/1‑71 (MQ=255)                    cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                                >  1:2404714/1‑71 (MQ=255)                    cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                                >  1:4167049/1‑71 (MQ=255)                    cGGGGATTTCAATGCGCCTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                                          <  1:345052/61‑1 (MQ=255)                     ggggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCg                                               >  1:11578/1‑71 (MQ=255)                        gATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAg                                                         >  1:4070724/1‑58 (MQ=255)                         aTTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGcc                                             <  1:957240/69‑1 (MQ=255)                         aTTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAg                                           >  1:4179011/1‑71 (MQ=255)                          tttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAgg                                          >  1:2737780/1‑71 (MQ=255)                          tttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAgg                                          >  1:4826648/1‑71 (MQ=255)                           ttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCAt                                                    <  1:3018891/60‑1 (MQ=255)                                tGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                                     >  1:196897/1‑70 (MQ=255)                                tGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAAt                                    <  1:492960/71‑1 (MQ=255)                                 gcgcGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCa                                            >  1:3699689/1‑62 (MQ=255)                                  cgcgTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGccc                                                      >  1:3348645/1‑51 (MQ=255)                                     gtgaTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATATCAt                               >  1:4427525/4‑71 (MQ=255)                                      tAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCt                                       <  1:3508119/62‑1 (MQ=255)                                      tAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCt                                       <  1:3640485/62‑1 (MQ=255)                                       aaTTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                                          >  1:3765145/1‑42 (MQ=255)                                        aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCa                                                     >  1:5094101/1‑46 (MQ=255)                                        aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCa                                                     >  1:3732216/1‑46 (MQ=255)                                        aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATAtcatcac                            <  1:4273078/71‑2 (MQ=255)                                        aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATAtcatcac                            <  1:2031314/71‑2 (MQ=255)                                        aTTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAAt                                    >  1:2499133/1‑63 (MQ=255)                                         tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                                             <  1:3443255/37‑1 (MQ=255)                                         tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                                             <  1:4969394/37‑1 (MQ=255)                                         tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                                     >  1:937381/1‑61 (MQ=255)                                         tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                                     >  1:655023/1‑61 (MQ=255)                                         tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                                     >  1:2579874/1‑61 (MQ=255)                                         tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                                     >  1:4457961/1‑61 (MQ=255)                                         tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                                     >  1:315219/1‑61 (MQ=255)                                           tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCa                                                  >  1:93137/1‑46 (MQ=255)                                           tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGTCCTAATACCATCAGTgg                         >  1:199680/1‑71 (MQ=255)                                           tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTgg                         >  1:542266/1‑71 (MQ=255)                                              gACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCag                      <  1:63939/71‑1 (MQ=255)                                               aCATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCa                       >  1:2817230/1‑69 (MQ=255)                                                  tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa                  <  1:4152717/71‑1 (MQ=255)                                                  tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa                  <  1:1431740/71‑1 (MQ=255)                                                  tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa                  <  1:496719/71‑1 (MQ=255)                                                    gCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaag                 <  1:2664782/70‑1 (MQ=255)                                                    gCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa                  >  1:2964123/1‑69 (MQ=255)                                                      aaCCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCagag                    <  1:970742/65‑1 (MQ=255)                                                        ccGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGa            <  1:3441930/71‑1 (MQ=255)                                                         cGGTTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGAc           >  1:3428610/1‑71 (MQ=255)                                                         cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATAcc                                 >  1:4291826/1‑49 (MQ=255)                                                         cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaaga                >  1:4803228/1‑66 (MQ=255)                                                         cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaaga                >  1:3739576/1‑66 (MQ=255)                                                         cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaaga                >  1:3224927/1‑66 (MQ=255)                                                         cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGAc           >  1:2630935/1‑71 (MQ=255)                                                         cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGAc           >  1:1983394/1‑71 (MQ=255)                                                           ggTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCa                             >  1:4381831/1‑51 (MQ=255)                                                                  aaaCAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTg  >  1:3501221/1‑71 (MQ=255)                                                                   aaCAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTg  <  1:4135773/70‑1 (MQ=255)                                                                     |                                                                    GTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTG  >  minE/453841‑453976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑