Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 454,692 T→C 16.6% P131P (CCA→CCG) ‡ ybgH ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE454,6920TC16.6% 223.7 / 35.0 102P131P (CCA→CCG) ‡ybgHpredicted transporter
Reads supporting (aligned to +/- strand):  ref base T (38/47);  new base C (9/8);  total (47/55)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01

Only 100 of 104 total aligned reads displayed.

GTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATA  >  minE/454622‑454758
                                                                      |                                                                  
gTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGtt                                                                    >  1:2602373/1‑71 (MQ=255)
      taataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                              >  1:4584737/1‑71 (MQ=255)
      taataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                              >  1:4852968/1‑71 (MQ=255)
      taataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                              <  1:1288495/71‑1 (MQ=255)
        ataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCAt                                                             <  1:5206795/70‑1 (MQ=255)
           gACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAg                                                         >  1:1597261/1‑71 (MQ=255)
              cccACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGc                                                                 <  1:2548731/60‑1 (MQ=255)
              cccACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc                                                        <  1:3234855/69‑1 (MQ=255)
               ccACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTc                                                      <  1:5219523/70‑1 (MQ=255)
                  cGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGcc                                                   >  1:347294/1‑70 (MQ=255)
                  cGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCg                                                  <  1:5280789/71‑1 (MQ=255)
                   gTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGtt                                                                    <  1:1217606/52‑1 (MQ=255)
                    ttACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                              <  1:4590272/57‑1 (MQ=255)
                    ttACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                <  1:2071261/71‑1 (MQ=255)
                     tACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATa                                                            >  1:309591/1‑58 (MQ=255)
                     tACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                >  1:261339/1‑70 (MQ=255)
                     tACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                >  1:4880521/1‑70 (MQ=255)
                      gcccGCAGCATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGc                                               >  1:1873060/2‑70 (MQ=255)
                       cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                              <  1:3502401/70‑1 (MQ=255)
                       cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                              <  1:2095070/70‑1 (MQ=255)
                       cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                              <  1:3495535/70‑1 (MQ=255)
                       cccGCAGCATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCa                                              <  1:4836805/70‑1 (MQ=255)
                        ccgccgCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                              <  1:3817972/53‑1 (MQ=255)
                            agcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAAc                                           <  1:5091729/67‑1 (MQ=255)
                            agcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAg                                         >  1:4744271/2‑70 (MQ=255)
                              cATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCt                                       >  1:4100189/1‑70 (MQ=255)
                               aTACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtga                                     >  1:49635/1‑69 (MQ=255)
                                tACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                              <  1:4205409/45‑1 (MQ=255)
                                tACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                              <  1:2830171/45‑1 (MQ=255)
                                tACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                <  1:3852371/59‑1 (MQ=255)
                                tACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                <  1:5309415/59‑1 (MQ=255)
                                tACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtgat                                    >  1:3753874/1‑68 (MQ=255)
                                tACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtgat                                    >  1:296372/1‑68 (MQ=255)
                                  cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                              >  1:1126806/1‑59 (MQ=255)
                                  cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                  >  1:1176694/1‑71 (MQ=255)
                                  cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                  >  1:4004165/1‑71 (MQ=255)
                                   aTCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                 <  1:3866004/71‑1 (MQ=255)
                                   aTCAGCGAGAAAACGCCATCACGACGCGGATCGGTTGGCTCa                                                              <  1:5260130/42‑1 (MQ=255)
                                    tCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                              >  1:5491708/1‑57 (MQ=255)
                                     cAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGttt                                >  1:4326694/1‑69 (MQ=255)
                                      aGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATa                                                            >  1:1199174/1‑41 (MQ=255)
                                      aGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATa                                                            >  1:3734168/1‑41 (MQ=255)
                                      aGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAg                                                         >  1:593407/1‑44 (MQ=255)
                                      aGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGa                              <  1:1171842/71‑1 (MQ=255)
                                       gCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc                                                        >  1:4334390/1‑44 (MQ=255)
                                         gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                            >  1:4849648/1‑70 (MQ=255)
                                         gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                            >  1:2078218/1‑70 (MQ=255)
                                         gaaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAttt                           <  1:1086908/68‑1 (MQ=255)
                                          agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAg                                                         <  1:1334661/40‑1 (MQ=255)
                                          agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                          <  1:3817507/71‑1 (MQ=255)
                                          agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                          <  1:742179/71‑1 (MQ=255)
                                          agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                          <  1:1105808/71‑1 (MQ=255)
                                          agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                          <  1:1109709/71‑1 (MQ=255)
                                          aaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttg                          <  1:1687386/69‑2 (MQ=255)
                                           aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttga                         <  1:3393136/70‑3 (MQ=255)
                                           aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttga                         <  1:1109761/70‑3 (MQ=255)
                                           aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttg                          >  1:674426/2‑69 (MQ=255)
                                            aaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtga                                     >  1:1477802/1‑56 (MQ=255)
                                            aaaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                          >  1:2577328/1‑69 (MQ=255)
                                             aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                        <  1:1567786/70‑1 (MQ=255)
                                             aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAAc                       <  1:5627258/71‑1 (MQ=255)
                                             aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAAc                       <  1:5510105/71‑1 (MQ=255)
                                              aCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                        >  1:3771252/1‑69 (MQ=255)
                                              aCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                        >  1:2965054/1‑69 (MQ=255)
                                               ccGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGATTTgaataa                     <  1:3078987/71‑4 (MQ=255)
                                                 gCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAttt                           >  1:1372152/1‑63 (MQ=255)
                                                  ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                                    >  1:5221943/1‑53 (MQ=255)
                                                  ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                                    >  1:5176542/1‑53 (MQ=255)
                                                  ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                                    >  1:1110962/1‑53 (MQ=255)
                                                  ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                            <  1:3610407/61‑1 (MQ=255)
                                                   cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                >  1:3695262/1‑40 (MQ=255)
                                                   cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                        >  1:696834/1‑64 (MQ=255)
                                                   cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                        >  1:2182490/1‑64 (MQ=255)
                                                     tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGAc                                           <  1:4230463/43‑1 (MQ=255)
                                                     tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                 <  1:3970061/53‑1 (MQ=255)
                                                     tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                 <  1:4060548/53‑1 (MQ=255)
                                                     tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                          <  1:3263689/60‑1 (MQ=255)
                                                      cACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGAc                                           <  1:3938897/42‑1 (MQ=255)
                                                       acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                <  1:2997829/36‑1 (MQ=255)
                                                       acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                            >  1:2797306/1‑40 (MQ=255)
                                                       acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                         >  1:2279261/1‑43 (MQ=255)
                                                       acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                         >  1:2915267/1‑43 (MQ=255)
                                                       acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                       <  1:3711023/45‑1 (MQ=255)
                                                       acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                       <  1:321950/45‑1 (MQ=255)
                                                       acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                       <  1:3996830/45‑1 (MQ=255)
                                                       acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                       <  1:2100564/45‑1 (MQ=255)
                                                       acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGc             >  1:3275336/1‑71 (MQ=255)
                                                         gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg           <  1:1205343/71‑1 (MQ=255)
                                                         gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg           >  1:3473341/1‑71 (MQ=255)
                                                          acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                  <  1:2854758/47‑1 (MQ=255)
                                                          acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaa                         <  1:2667699/56‑1 (MQ=255)
                                                               gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                        >  1:2236478/1‑52 (MQ=255)
                                                               gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                          <  1:5402684/50‑1 (MQ=255)
                                                               gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACg     <  1:1842496/71‑1 (MQ=255)
                                                                aTCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAg                               <  1:163790/44‑1 (MQ=255)
                                                                 tCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGAt   <  1:4243344/71‑1 (MQ=255)
                                                                  cGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                             >  1:4519623/1‑44 (MQ=255)
                                                                  cGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                             >  1:692470/1‑44 (MQ=255)
                                                                   ggTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                                     >  1:3884009/1‑35 (MQ=255)
                                                                   ggTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATa  <  1:3777455/70‑1 (MQ=255)
                                                                      |                                                                  
GTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATA  >  minE/454622‑454758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑