Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 454,694 G→C 16.8% P131A (CCA→GCA) ‡ ybgH ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE454,6940GC16.8% 233.3 / 36.8 101P131A (CCA→GCA) ‡ybgHpredicted transporter
Reads supporting (aligned to +/- strand):  ref base G (37/47);  new base C (9/8);  total (46/55)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 104 total aligned reads displayed.

TAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGC  >  minE/454628‑454763
                                                                  |                                                                     
taataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                                   >  1:4852968/1‑71 (MQ=255)
taataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                                   >  1:4584737/1‑71 (MQ=255)
taataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                                   <  1:1288495/71‑1 (MQ=255)
  ataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCAt                                                                  <  1:5206795/70‑1 (MQ=255)
     gACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAg                                                              >  1:1597261/1‑71 (MQ=255)
        cccACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGc                                                                      <  1:2548731/60‑1 (MQ=255)
        cccACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc                                                             <  1:3234855/69‑1 (MQ=255)
         ccACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTc                                                           <  1:5219523/70‑1 (MQ=255)
            cGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGcc                                                        >  1:347294/1‑70 (MQ=255)
            cGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCg                                                       <  1:5280789/71‑1 (MQ=255)
              ttACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                                   <  1:4590272/57‑1 (MQ=255)
              ttACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                     <  1:2071261/71‑1 (MQ=255)
               tACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATa                                                                 >  1:309591/1‑58 (MQ=255)
               tACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                     >  1:4880521/1‑70 (MQ=255)
               tACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                     >  1:261339/1‑70 (MQ=255)
                gcccGCAGCATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGc                                                    >  1:1873060/2‑70 (MQ=255)
                 cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                                   <  1:3495535/70‑1 (MQ=255)
                 cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                                   <  1:2095070/70‑1 (MQ=255)
                 cccGCAGCATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCa                                                   <  1:4836805/70‑1 (MQ=255)
                  ccgccgCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                                   <  1:3817972/53‑1 (MQ=255)
                      agcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAAc                                                <  1:5091729/67‑1 (MQ=255)
                      agcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAg                                              >  1:4744271/2‑70 (MQ=255)
                        cATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCt                                            >  1:4100189/1‑70 (MQ=255)
                         aTACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtga                                          >  1:49635/1‑69 (MQ=255)
                          tACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                                   <  1:4205409/45‑1 (MQ=255)
                          tACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa                                                                   <  1:2830171/45‑1 (MQ=255)
                          tACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                     <  1:5309415/59‑1 (MQ=255)
                          tACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                     <  1:3852371/59‑1 (MQ=255)
                          tACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtgat                                         >  1:3753874/1‑68 (MQ=255)
                          tACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtgat                                         >  1:296372/1‑68 (MQ=255)
                            cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                                   >  1:1126806/1‑59 (MQ=255)
                            cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                       >  1:4004165/1‑71 (MQ=255)
                            cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                       >  1:1176694/1‑71 (MQ=255)
                             aTCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                      <  1:3866004/71‑1 (MQ=255)
                             aTCAGCGAGAAAACGCCATCACGACGCGGATCGGTTGGCTCa                                                                   <  1:5260130/42‑1 (MQ=255)
                              tCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                                   >  1:5491708/1‑57 (MQ=255)
                               cAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGttt                                     >  1:4326694/1‑69 (MQ=255)
                                aGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATa                                                                 >  1:3734168/1‑41 (MQ=255)
                                aGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATa                                                                 >  1:1199174/1‑41 (MQ=255)
                                aGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAg                                                              >  1:593407/1‑44 (MQ=255)
                                aGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGa                                   <  1:1171842/71‑1 (MQ=255)
                                 gCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc                                                             >  1:4334390/1‑44 (MQ=255)
                                   gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                 >  1:4849648/1‑70 (MQ=255)
                                   gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                 >  1:2078218/1‑70 (MQ=255)
                                   gaaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAttt                                <  1:1086908/68‑1 (MQ=255)
                                    agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAg                                                              <  1:1334661/40‑1 (MQ=255)
                                    agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                               <  1:3817507/71‑1 (MQ=255)
                                    agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                               <  1:1105808/71‑1 (MQ=255)
                                    agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                               <  1:1109709/71‑1 (MQ=255)
                                    agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                               <  1:2163150/71‑1 (MQ=255)
                                    aaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttg                               <  1:1687386/69‑2 (MQ=255)
                                     aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttga                              <  1:3393136/70‑3 (MQ=255)
                                     aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttga                              <  1:1109761/70‑3 (MQ=255)
                                     aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttg                               >  1:674426/2‑69 (MQ=255)
                                      aaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtga                                          >  1:1477802/1‑56 (MQ=255)
                                      aaaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                               >  1:2577328/1‑69 (MQ=255)
                                       aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                             <  1:1567786/70‑1 (MQ=255)
                                       aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAAc                            <  1:5510105/71‑1 (MQ=255)
                                       aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAAc                            <  1:5627258/71‑1 (MQ=255)
                                        aCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                             >  1:2965054/1‑69 (MQ=255)
                                        aCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                             >  1:3771252/1‑69 (MQ=255)
                                         ccGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGATTTgaataa                          <  1:3078987/71‑4 (MQ=255)
                                           gCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAttt                                >  1:1372152/1‑63 (MQ=255)
                                            ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                                         >  1:1110962/1‑53 (MQ=255)
                                            ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                                         >  1:5221943/1‑53 (MQ=255)
                                            ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                                         >  1:5176542/1‑53 (MQ=255)
                                            ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                 <  1:3610407/61‑1 (MQ=255)
                                             cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                     >  1:3695262/1‑40 (MQ=255)
                                             cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                             >  1:696834/1‑64 (MQ=255)
                                               tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGAc                                                <  1:4230463/43‑1 (MQ=255)
                                               tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                      <  1:4060548/53‑1 (MQ=255)
                                               tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                      <  1:3970061/53‑1 (MQ=255)
                                               tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                               <  1:3263689/60‑1 (MQ=255)
                                                cACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGAc                                                <  1:3938897/42‑1 (MQ=255)
                                                 acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                     <  1:2997829/36‑1 (MQ=255)
                                                 acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                                 >  1:2797306/1‑40 (MQ=255)
                                                 acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                              >  1:2279261/1‑43 (MQ=255)
                                                 acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                              >  1:2915267/1‑43 (MQ=255)
                                                 acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                            <  1:3996830/45‑1 (MQ=255)
                                                 acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                            <  1:2100564/45‑1 (MQ=255)
                                                 acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                            <  1:321950/45‑1 (MQ=255)
                                                 acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                            <  1:3711023/45‑1 (MQ=255)
                                                 acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGc                  >  1:3275336/1‑71 (MQ=255)
                                                   gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg                >  1:3473341/1‑71 (MQ=255)
                                                   gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg                <  1:1205343/71‑1 (MQ=255)
                                                    acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                       <  1:3498253/47‑1 (MQ=255)
                                                    acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                       <  1:2854758/47‑1 (MQ=255)
                                                    acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaa                              <  1:2667699/56‑1 (MQ=255)
                                                         gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                             >  1:2236478/1‑52 (MQ=255)
                                                         gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                               <  1:5402684/50‑1 (MQ=255)
                                                         gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACg          <  1:1842496/71‑1 (MQ=255)
                                                          aTCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAg                                    <  1:163790/44‑1 (MQ=255)
                                                           tCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGAt        <  1:4243344/71‑1 (MQ=255)
                                                            cGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                  >  1:4519623/1‑44 (MQ=255)
                                                            cGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                  >  1:692470/1‑44 (MQ=255)
                                                             ggTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                                          >  1:3884009/1‑35 (MQ=255)
                                                             ggTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATa       <  1:3777455/70‑1 (MQ=255)
                                                                 ggCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc  >  1:3050148/1‑71 (MQ=255)
                                                                 ggCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc  >  1:5072854/1‑71 (MQ=255)
                                                                  gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc  <  1:4311849/70‑1 (MQ=255)
                                                                  |                                                                     
TAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGC  >  minE/454628‑454763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑