| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 454,698 | A→G | 17.3% | Y129Y (TAT→TAC) | ybgH ← | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 454,698 | 0 | A | G | 17.3% | 213.9 / 34.0 | 98 | Y129Y (TAT→TAC) | ybgH | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (40/41); new base G (9/8); total (49/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 109 total aligned reads displayed.
TAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGG > minE/454628‑454767 | taataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa > 1:4584737/1‑71 (MQ=255)taataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa < 1:1288495/71‑1 (MQ=255) ataGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCAt < 1:5206795/70‑1 (MQ=255) gACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAg > 1:1597261/1‑71 (MQ=255) cccACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc < 1:3234855/69‑1 (MQ=255) ccACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTc < 1:5219523/70‑1 (MQ=255) cGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGcc > 1:347294/1‑70 (MQ=255) cGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCg < 1:5280789/71‑1 (MQ=255) ttACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa < 1:4590272/57‑1 (MQ=255) ttACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg < 1:2071261/71‑1 (MQ=255) tACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATa > 1:309591/1‑58 (MQ=255) tACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg > 1:261339/1‑70 (MQ=255) tACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg > 1:4880521/1‑70 (MQ=255) gcccGCAGCATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGc > 1:1873060/2‑70 (MQ=255) cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa < 1:3502401/70‑1 (MQ=255) cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa < 1:2095070/70‑1 (MQ=255) cccGCAGCATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCa < 1:4836805/70‑1 (MQ=255) ccgccgCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa < 1:3817972/53‑1 (MQ=255) agcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAAc < 1:5091729/67‑1 (MQ=255) agcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAg > 1:4744271/2‑70 (MQ=255) cATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCt > 1:4100189/1‑70 (MQ=255) aTACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtga > 1:49635/1‑69 (MQ=255) tACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa < 1:2830171/45‑1 (MQ=255) tACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCa < 1:4205409/45‑1 (MQ=255) tACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg < 1:5309415/59‑1 (MQ=255) tACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtgat > 1:3753874/1‑68 (MQ=255) tACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtgat > 1:296372/1‑68 (MQ=255) cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa > 1:1126806/1‑59 (MQ=255) cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt > 1:1176694/1‑71 (MQ=255) cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt > 1:4004165/1‑71 (MQ=255) aTCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt < 1:3866004/71‑1 (MQ=255) tCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa > 1:5491708/1‑57 (MQ=255) cAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGttt > 1:4326694/1‑69 (MQ=255) aGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATa > 1:3734168/1‑41 (MQ=255) aGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATa > 1:1199174/1‑41 (MQ=255) aGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAg > 1:593407/1‑44 (MQ=255) aGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGa < 1:1171842/71‑1 (MQ=255) gCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGc > 1:4334390/1‑44 (MQ=255) gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt > 1:4849648/1‑70 (MQ=255) gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt > 1:4574586/1‑70 (MQ=255) gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt > 1:2078218/1‑70 (MQ=255) gaaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAttt < 1:1086908/68‑1 (MQ=255) agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAg < 1:1334661/40‑1 (MQ=255) agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa < 1:3817507/71‑1 (MQ=255) agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa < 1:1105808/71‑1 (MQ=255) agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa < 1:1109709/71‑1 (MQ=255) aaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttg < 1:1687386/69‑2 (MQ=255) aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttga < 1:1109761/70‑3 (MQ=255) aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttga < 1:3393136/70‑3 (MQ=255) aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttg > 1:674426/2‑69 (MQ=255) aaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtga > 1:1477802/1‑56 (MQ=255) aaaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa > 1:2577328/1‑69 (MQ=255) aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa < 1:1567786/70‑1 (MQ=255) aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAAc < 1:5627258/71‑1 (MQ=255) aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAAc < 1:5510105/71‑1 (MQ=255) aCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa > 1:3771252/1‑69 (MQ=255) ccGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGATTTgaataa < 1:3078987/71‑4 (MQ=255) ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc > 1:5176542/1‑53 (MQ=255) ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc > 1:5221943/1‑53 (MQ=255) ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc > 1:1110962/1‑53 (MQ=255) ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt < 1:3610407/61‑1 (MQ=255) cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg > 1:3695262/1‑40 (MQ=255) cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa > 1:696834/1‑64 (MQ=255) cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa > 1:2182490/1‑64 (MQ=255) tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt < 1:4060548/53‑1 (MQ=255) tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt < 1:3970061/53‑1 (MQ=255) tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa < 1:3263689/60‑1 (MQ=255) cACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGAc < 1:3938897/42‑1 (MQ=255) acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAg < 1:2997829/36‑1 (MQ=255) acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa > 1:2797306/1‑40 (MQ=255) acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg > 1:2915267/1‑43 (MQ=255) acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg > 1:2279261/1‑43 (MQ=255) acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt < 1:3996830/45‑1 (MQ=255) acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt < 1:321950/45‑1 (MQ=255) acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt < 1:3711023/45‑1 (MQ=255) acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt < 1:2100564/45‑1 (MQ=255) acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGc > 1:3275336/1‑71 (MQ=255) gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg < 1:1205343/71‑1 (MQ=255) gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg > 1:3473341/1‑71 (MQ=255) acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt < 1:3498253/47‑1 (MQ=255) acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt < 1:2854758/47‑1 (MQ=255) acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaa < 1:2667699/56‑1 (MQ=255) gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa > 1:2236478/1‑52 (MQ=255) gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa < 1:5402684/50‑1 (MQ=255) gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACg < 1:1842496/71‑1 (MQ=255) aTCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAg < 1:163790/44‑1 (MQ=255) tCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGAt < 1:4243344/71‑1 (MQ=255) cGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt > 1:692470/1‑44 (MQ=255) cGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt > 1:4519623/1‑44 (MQ=255) ggTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa > 1:3884009/1‑35 (MQ=255) ggTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATa < 1:3777455/70‑1 (MQ=255) ggCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc > 1:5072854/1‑71 (MQ=255) ggCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc > 1:3050148/1‑71 (MQ=255) gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc < 1:4311849/70‑1 (MQ=255) tCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGAc > 1:5183545/1‑59 (MQ=255) cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg > 1:584049/1‑71 (MQ=255) cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg > 1:5188323/1‑71 (MQ=255) aTACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa > 1:4143060/1‑39 (MQ=255) aTACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaa < 1:5125345/38‑1 (MQ=255) aTACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATa < 1:418905/61‑1 (MQ=255) | TAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGG > minE/454628‑454767 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |