BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	454,722	T→G	8.4%	V121V (GTA→GTC) ‡	ybgH ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	454,722	0	T	G	8.4%	238.5 / 15.3	95	V121V (GTA→GTC) ‡	ybgH	predicted transporter
Reads supporting (aligned to +/- strand): ref base T (47/40); new base G (2/6); total (49/46)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 101 total aligned reads displayed.

CATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGA  >  minE/454656‑454788

cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                                                >  1:4004165/1‑71 (MQ=255)

cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                                                >  1:1176694/1‑71 (MQ=255)

 aTCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                                               <  1:3866004/71‑1 (MQ=255)

   cAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGttt                                                              >  1:4326694/1‑69 (MQ=255)

    aGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGa                                                            <  1:1171842/71‑1 (MQ=255)

       gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                                          >  1:4574586/1‑70 (MQ=255)

       gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                                          >  1:4849648/1‑70 (MQ=255)

       gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                                          >  1:2078218/1‑70 (MQ=255)

       gaaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAttt                                                         <  1:1086908/68‑1 (MQ=255)

        agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                        <  1:1105808/71‑1 (MQ=255)

        agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                        <  1:3817507/71‑1 (MQ=255)

        agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                        <  1:2163150/71‑1 (MQ=255)

        agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                        <  1:742179/71‑1 (MQ=255)

        agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                        <  1:1109709/71‑1 (MQ=255)

        aaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttg                                                        <  1:1687386/69‑2 (MQ=255)

         aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttga                                                       <  1:3393136/70‑3 (MQ=255)

         aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttga                                                       <  1:1109761/70‑3 (MQ=255)

         aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttg                                                        >  1:674426/2‑69 (MQ=255)

          aaaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                        >  1:2577328/1‑69 (MQ=255)

           aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                                      <  1:1567786/70‑1 (MQ=255)

           aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAAc                                                     <  1:5510105/71‑1 (MQ=255)

           aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAAc                                                     <  1:5627258/71‑1 (MQ=255)

            aCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                                      >  1:2965054/1‑69 (MQ=255)

            aCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                                      >  1:3771252/1‑69 (MQ=255)

             ccGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGATTTgaataa                                                   <  1:3078987/71‑4 (MQ=255)

               gCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAttt                                                         >  1:1372152/1‑63 (MQ=255)

                ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                                                                  >  1:1110962/1‑53 (MQ=255)

                ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                                                                  >  1:5176542/1‑53 (MQ=255)

                ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                                                                  >  1:5221943/1‑53 (MQ=255)

                ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                                          <  1:3610407/61‑1 (MQ=255)

                 cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                                      >  1:2182490/1‑64 (MQ=255)

                 cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                                      >  1:696834/1‑64 (MQ=255)

                   tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                                               <  1:3970061/53‑1 (MQ=255)

                   tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                                               <  1:4060548/53‑1 (MQ=255)

                   tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                        <  1:3263689/60‑1 (MQ=255)

                     acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGc                                           >  1:3275336/1‑71 (MQ=255)

                       gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg                                         >  1:3473341/1‑71 (MQ=255)

                       gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg                                         <  1:1205343/71‑1 (MQ=255)

                        acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                                                <  1:2854758/47‑1 (MQ=255)

                        acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                                                <  1:3498253/47‑1 (MQ=255)

                        acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaa                                                       <  1:2667699/56‑1 (MQ=255)

                             gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                                      >  1:2236478/1‑52 (MQ=255)

                             gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                        <  1:5402684/50‑1 (MQ=255)

                             gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACg                                   <  1:1842496/71‑1 (MQ=255)

                              aTCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAg                                                             <  1:163790/44‑1 (MQ=255)

                               tCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGAt                                 <  1:4243344/71‑1 (MQ=255)

                                cGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                                           >  1:692470/1‑44 (MQ=255)

                                cGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                                           >  1:4519623/1‑44 (MQ=255)

                                 ggTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                                                                   >  1:3884009/1‑35 (MQ=255)

                                 ggTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATa                                <  1:3777455/70‑1 (MQ=255)

                                     ggCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc                           >  1:5072854/1‑71 (MQ=255)

                                     ggCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc                           >  1:3050148/1‑71 (MQ=255)

                                      gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc                           <  1:4311849/70‑1 (MQ=255)

                                        tCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGAc                                    >  1:5183545/1‑59 (MQ=255)

                                         cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg                       >  1:584049/1‑71 (MQ=255)

                                         cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg                       >  1:5188323/1‑71 (MQ=255)

                                          aTACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                                      >  1:4143060/1‑39 (MQ=255)

                                          aTACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaa                                                       <  1:5125345/38‑1 (MQ=255)

                                          aTACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATa                                <  1:418905/61‑1 (MQ=255)

                                           tACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                        <  1:2368459/36‑1 (MQ=255)

                                           tACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                        <  1:5052421/36‑1 (MQ=255)

                                           tACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                        <  1:2009076/36‑1 (MQ=255)

                                           tACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg                                         <  1:4566114/51‑1 (MQ=255)

                                           tACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa                     <  1:947705/71‑1 (MQ=255)

                                            aCAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATa                                >  1:3571251/1‑59 (MQ=255)

                                            aCAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa                     >  1:635432/1‑70 (MQ=255)

                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAAc                                                     >  1:3553263/1‑37 (MQ=255)

                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCAt                                              >  1:5172477/1‑44 (MQ=255)

                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCAt                                              >  1:5319322/1‑44 (MQ=255)

                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCAt                                              >  1:3776662/1‑44 (MQ=255)

                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCAt                                              >  1:1600839/1‑44 (MQ=255)

                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATc                             >  1:2676194/1‑61 (MQ=255)

                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAg                        >  1:647220/1‑66 (MQ=255)

                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAg                        >  1:3311335/1‑66 (MQ=255)

                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa                     >  1:1643201/1‑69 (MQ=255)

                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa                     >  1:5314031/1‑69 (MQ=255)

                                               gCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGa                 <  1:1723237/71‑1 (MQ=255)

                                               gCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGa                 <  1:908907/71‑1 (MQ=255)

                                                   gCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAAt                              >  1:493776/1‑54 (MQ=255)

                                                   gCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATag              <  1:2707101/70‑1 (MQ=255)

                                                   gCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATa               >  1:4708379/1‑69 (MQ=255)

                                                   gCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATa               >  1:2761581/1‑69 (MQ=255)

                                                   gCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATa               <  1:4056450/69‑1 (MQ=255)

                                                      gAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAgg           >  1:1565390/1‑70 (MQ=255)

                                                        gCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAAt        <  1:1799607/71‑1 (MQ=255)

                                                          aGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGa      >  1:358902/1‑71 (MQ=255)

                                                            aCAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGa                 <  1:4460112/58‑1 (MQ=255)

                                                             cAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAg                  <  1:402183/56‑1 (MQ=255)

                                                              aGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACg    >  1:1554665/1‑69 (MQ=255)

                                                              aGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACg    >  1:3121675/1‑69 (MQ=255)

                                                              aGCTTAAGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACgga  >  1:9035/1‑71 (MQ=255)

                                                               gCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGcc                          <  1:668650/46‑1 (MQ=255)

                                                                cTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGcc                          <  1:4472244/45‑1 (MQ=255)

                                                                cTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGcc                          >  1:1578453/1‑45 (MQ=255)

                                                                 ttACGTTAGATTTAAACAGGCCATAGCCGCAGACGa                                  <  1:195081/36‑1 (MQ=255)

                                                                 ttACGTTAGATTTAAACAGGCCATAGCCGCAGACGa                                  <  1:4998719/36‑1 (MQ=255)

                                                                 ttACGTTAGATTTAAACAGGCCATAGCCGCAGACGa                                  <  1:4994634/36‑1 (MQ=255)

                                                                 ttACGTTAGATTTAAACAGGCCATAGCCGCAGACGa                                  <  1:4925630/36‑1 (MQ=255)

                                                                 ttACGTTAGATTTAAACAGGCCATAGCCGCAGACGa                                  <  1:4918871/36‑1 (MQ=255)

                                                                 ttACGTTAGATTTAAACAGGCCATAGCCGCAGACGa                                  <  1:3067692/36‑1 (MQ=255)

CATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGA  >  minE/454656‑454788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`