| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 457,686 | C→T | 23.2% | L306L (CTG→TTG) | ybgK → | predicted enzyme subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 457,686 | 0 | C | T | 23.2% | 127.5 / 32.0 | 69 | L306L (CTG→TTG) | ybgK | predicted enzyme subunit |
| Reads supporting (aligned to +/- strand): ref base C (32/21); new base T (13/3); total (45/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGC > minE/457617‑457756 | tgtTCACTGGAAGAGG‑ACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTg > 1:2129220/1‑71 (MQ=255) ttCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTg > 1:703415/1‑70 (MQ=255) ggAAGAGGCACTGAAAGCACGGCAAGATCAACAACGTTATTTCGAACAATTAGCGTGGCGGCTGCACAAt > 1:793842/1‑70 (MQ=255) ggAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAAt > 1:2958328/1‑70 (MQ=255) gagcttcagtatgatcccaaagtttatgatgaatatcgtaagacgaagcgggcggGGCGGTTGAACAATGa > 1:915889‑M1/56‑71 (MQ=255) gAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCt < 1:4350144/62‑1 (MQ=255) gAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTT‑AACAATTAGCGTGGCGGCTGCACAATGaa < 1:1114765/71‑1 (MQ=255) agcttcagtatgatcccaaagtttatgatgaatatcgtaagacgaagcgggcggGGCGGTTGAACAATGa < 1:2236549‑M1/16‑1 (MQ=255) gcttcagtatgatcccaaagtttatgatgaatatcgtaagacgaagcgggcggGGCGGTTGAACAATGaaa > 1:3717041‑M1/54‑71 (MQ=255) gcttcagtatgatcccaaagtttatgatgaatatcgtaagacgaagcgggcggGGCGGTTGAACAATGaaa > 1:2977298‑M1/54‑71 (MQ=255) gcttcagtatgatcccaaagtttatgatgaatatcgtaagacgaagcgggcggGGCGGTTGAACAATGaaa > 1:3710344‑M1/54‑71 (MQ=255) gcttcagtatgatcccaaagtttatgatgaatatcgtaagacgaagcgggcggGGCGGTTGAACAATGa > 1:240379‑M1/54‑69 (MQ=255) gagGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGaaaa < 1:3948132/71‑1 (MQ=255) cttcagtatgatcccaaagtttatgatgaatatcgtaagacgaagcgggcggGGCGGTTGAACAATGaaaa > 1:297011‑M1/53‑71 (MQ=255) tcagtatgatcccaaagtttatgatgaatatcgtaagacgaagcgggcggGGCGGTTGAACAATGAAAAt < 1:5264732‑M1/20‑1 (MQ=255) gCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTg < 1:3112133/71‑1 (MQ=255) gCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTg < 1:1744701/71‑1 (MQ=255) gtatgatcccaaagtttatgatgaatatcgtaagacgaagcgggcggGGCGGTTGAACAATGAAAatc > 1:2557310‑M1/48‑67 (MQ=255) tatgatcccaaagtttatgatgaatatcgtaagacgaagcgggcggGGCGGTTGaaca > 1:2224193‑M1/47‑58 (MQ=255) cTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACaa < 1:5033258/59‑1 (MQ=255) cTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGaa < 1:205400/63‑1 (MQ=255) cTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGaa < 1:159483/63‑1 (MQ=255) tAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAAt > 1:5669189/1‑59 (MQ=255) tAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGa < 1:2727195/68‑1 (MQ=255) aaaaGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGaa < 1:4995752/61‑1 (MQ=255) aaaGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGAc > 1:1015479/1‑67 (MQ=255) aaaGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCt < 1:3095273/69‑1 (MQ=255) aaaGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTg > 1:856055/1‑70 (MQ=255) aGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAAc < 1:4796028/71‑1 (MQ=255) gCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGaaaa > 1:2377816/1‑59 (MQ=255) cGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGcc > 1:1941208/1‑70 (MQ=255) cGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGcc > 1:3988515/1‑70 (MQ=255) cGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCg < 1:471770/71‑1 (MQ=255) aagtttatgatgaatatcgtaagacgaagcgggcggGGCGGTTGAACAATGAAAAt > 1:1064855‑M1/37‑56 (MQ=255) cAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGAc > 1:4074634/1‑58 (MQ=255) aaGATCAGCAACGTTATTTTGAAGAATTAGCGTGGCGGCTGCACAATGAAAATTGACCt < 1:1424377/59‑1 (MQ=255) aaGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGAcc > 1:2918116/1‑58 (MQ=255) aaGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGAcc > 1:2400825/1‑58 (MQ=255) aaGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGAcc > 1:170246/1‑58 (MQ=255) aGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTg > 1:4701182/1‑71 (MQ=255) gATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGa < 1:5634265/47‑1 (MQ=255) gATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGa < 1:1830136/47‑1 (MQ=255) gATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGa < 1:3226732/47‑1 (MQ=255) aTCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGAc > 1:1799424/1‑54 (MQ=255) tCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGc > 1:971858/1‑71 (MQ=255) cAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGc > 1:739114/1‑70 (MQ=255) aaCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAgg > 1:2989288/1‑71 (MQ=255) gTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTgg < 1:3048413/61‑1 (MQ=255) atcgtaagacgaagcgggcggGGCGGTTGAACAATGAAAAt > 1:2239276‑M1/22‑41 (MQ=255) ttATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTgc < 1:1072977/71‑1 (MQ=255) tcgtaagacgaagcgggcggGGCGGTTGAACAATGa > 1:5139085‑M1/21‑36 (MQ=255) tcgtaagacgaagcgggcggGGCGGTTGAACAATGa > 1:3376426‑M1/21‑36 (MQ=255) tcgtaagacgaagcgggcggGGCGGTTGAACAATGa > 1:2228061‑M1/21‑36 (MQ=255) aTTTTGAACAATTAGCGTGGCGGCTGCACAATGaaa > 1:3144296/1‑36 (MQ=255) aTTTTGAACAATTAGCGTGGCGGCTGCACAATGaaa > 1:4838057/1‑36 (MQ=255) aTTTTGAACAATTAGCGTGGCGGCTGCACAATGaaa > 1:2802601/1‑36 (MQ=255) aTTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTgcgc > 1:2433623/1‑71 (MQ=255) aTTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTgcgc > 1:698919/1‑71 (MQ=255) ttttGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGa > 1:4298451/1‑40 (MQ=255) ttttGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGAc > 1:5107746/1‑41 (MQ=255) ttttGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGAc > 1:5306963/1‑41 (MQ=255) ttttGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATc > 1:2187942/1‑54 (MQ=255) aagacgaagcgggcggGGCGGTTGAACAATGAAAAt < 1:4967640‑M1/20‑1 (MQ=255) tGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAgg > 1:1472115/1‑61 (MQ=255) tGAACAATTAGCGTGGCGGCTCCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAgg > 1:3549911/1‑61 (MQ=255) aaTTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCt < 1:1749936/58‑1 (MQ=255) aaTTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCt < 1:793860/58‑1 (MQ=255) tAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCa > 1:3027193/1‑69 (MQ=255) aGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCagag > 1:3856260/1‑71 (MQ=255) ggcTGCACAATGAAAATTGACCTGAACGCCGATCTGGGGGAAGGCTGCGCCAGCGACGCAGAGCTATTAAc < 1:1462941/71‑1 (MQ=255) cTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGc < 1:107209/71‑1 (MQ=255) | TGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGC > minE/457617‑457756 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |