| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 509,811 | T→A | 13.5% | intergenic (+36/‑147) | ybhI → / → ybhJ | predicted transporter/predicted hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 509,811 | 0 | T | A | 13.5% | 127.7 / 12.1 | 52 | intergenic (+36/‑147) | ybhI/ybhJ | predicted transporter/predicted hydratase |
| Reads supporting (aligned to +/- strand): ref base T (22/23); new base A (4/3); total (26/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01 | |||||||||||
GTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCT > minE/509742‑509879 | gtggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtc > 1:2255932/1‑71 (MQ=255) tggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtct > 1:2917766/1‑71 (MQ=255) tggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtct < 1:5213687/71‑1 (MQ=255) tggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcggct < 1:3499539/71‑1 (MQ=255) gAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtctgc > 1:5389750/1‑71 (MQ=255) gAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtctg < 1:4563685/70‑1 (MQ=255) atatGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtctgcgt < 1:3158806/71‑1 (MQ=255) tatGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtctgcgt < 1:5195992/70‑1 (MQ=255) gCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGGGTTCGc > 1:1816370/1‑71 (MQ=255) gCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGc > 1:4312324/1‑71 (MQ=255) gATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtct < 1:4504083/60‑1 (MQ=255) aTCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAc > 1:4114385/1‑71 (MQ=255) tCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAcc < 1:5402810/71‑1 (MQ=255) cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTc > 1:4279611/1‑63 (MQ=255) cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTc > 1:1504264/1‑63 (MQ=255) cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:5059687/1‑71 (MQ=255) cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:3245123/1‑71 (MQ=255) cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:1641784/1‑71 (MQ=255) cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:2338810/1‑71 (MQ=255) ggCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc < 1:3040718/70‑1 (MQ=255) gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt < 1:5541444/69‑1 (MQ=255) gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt < 1:3622603/69‑1 (MQ=255) gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt < 1:390914/69‑1 (MQ=255) gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt < 1:5508383/69‑1 (MQ=255) gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCa < 1:5594135/71‑1 (MQ=255) gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCa > 1:5298674/1‑71 (MQ=255) aCATGCTGTAAATATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCact < 1:5525119/71‑1 (MQ=255) aCATGCTGTAAATATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCact < 1:3786743/71‑1 (MQ=255) aCATGCTGTAAATATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCact < 1:2539823/71‑1 (MQ=255) aTGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCactta < 1:4185844/71‑1 (MQ=255) aTGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCactta < 1:3844019/71‑1 (MQ=255) aTGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCactt > 1:3590426/1‑70 (MQ=255) aTGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAACCCAGTCactta < 1:5693374/71‑1 (MQ=255) tGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAcccc > 1:3472906/1‑61 (MQ=255) cTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCacttact < 1:5056631/70‑1 (MQ=255) tAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc < 1:1002421/55‑1 (MQ=255) tAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCActtactta < 1:1834590/69‑1 (MQ=255) aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCg > 1:5362582/1‑46 (MQ=255) aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGc > 1:861187/1‑47 (MQ=255) aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGc > 1:257486/1‑47 (MQ=255) aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:3156029/1‑53 (MQ=40) aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:2259062/1‑53 (MQ=40) aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGt > 1:3830634/1‑70 (MQ=39) aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGt < 1:3301971/70‑1 (MQ=39) aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGt < 1:4915030/70‑1 (MQ=39) atTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGc > 1:3927941/1‑71 (MQ=34) ttCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATg < 1:3969571/65‑1 (MQ=33) tctcGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt < 1:4111234/52‑1 (MQ=31) tctcGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt < 1:2971770/52‑1 (MQ=31) gTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGc < 1:3917907/71‑1 (MQ=32) tGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCtt > 1:1800773/1‑71 (MQ=34) tGCATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCtt > 1:2246424/1‑71 (MQ=11) gCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCtt < 1:4398392/70‑1 (MQ=35) cATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTg < 1:1322603/70‑1 (MQ=11) cATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTg < 1:4728494/70‑1 (MQ=11) cATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTg < 1:4247990/70‑1 (MQ=11) aTGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTc > 1:299285/1‑71 (MQ=34) tgtaCTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGTc > 1:4104332/1‑70 (MQ=11) tgtaCTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGTCg > 1:3145766/1‑71 (MQ=11) tgtaCTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGTCg > 1:2072234/1‑71 (MQ=11) tgctgcGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCg > 1:1710652/1‑69 (MQ=35) actgcgactgcgtTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGTCGCCt > 1:2545224/2‑71 (MQ=11) ctgcgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCt < 1:5105963/70‑1 (MQ=35) ctgcgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCt < 1:2113211/70‑1 (MQ=35) tgcgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGc > 1:2382228/1‑60 (MQ=33) cgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTcc < 1:2278782/70‑1 (MQ=35) cgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCt > 1:3089674/1‑71 (MQ=34) cgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCt > 1:2018292/1‑71 (MQ=34) cgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCt > 1:4230004/1‑71 (MQ=34) | GTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCT > minE/509742‑509879 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |