| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 533,864 | A→G | 5.9% | Y525H (TAT→CAT) ‡ | ybhF ← | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 533,864 | 0 | A | G | 5.9% | 195.0 / 2.2 | 68 | Y525H (TAT→CAT) ‡ | ybhF | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (37/27); new base G (2/2); total (39/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
ATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTG > minE/533802‑533933 | aTCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCt > 1:5619433/1‑70 (MQ=255) cGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCa < 1:4462223/71‑1 (MQ=255) cGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCa > 1:2966709/1‑71 (MQ=255) gCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCAt > 1:898690/1‑70 (MQ=255) gTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATaa < 1:2731463/70‑1 (MQ=255) gTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATa > 1:1264724/1‑69 (MQ=255) gTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATa > 1:5583975/1‑69 (MQ=255) gTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATa < 1:4489416/69‑1 (MQ=255) ccGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAgtg > 1:3844657/1‑71 (MQ=255) ccGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCAAAAAgt > 1:3957901/1‑70 (MQ=255) ggCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAgtgggtgg > 1:1589497/1‑71 (MQ=255) gCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGggtggt < 1:5581305/71‑1 (MQ=255) ttAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTc < 1:1840173/43‑1 (MQ=255) ttAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCa > 1:4450844/1‑50 (MQ=255) ttAATTTCACGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGAc > 1:3068870/1‑70 (MQ=255) tAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAata > 1:4129405/1‑39 (MQ=255) tAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAg > 1:353430/1‑58 (MQ=255) tAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAg > 1:3297072/1‑58 (MQ=255) tAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCa > 1:5470136/1‑71 (MQ=255) aaTTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCg > 1:106375/1‑43 (MQ=255) aaTTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCAt > 1:1894517/1‑71 (MQ=255) aTTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCAt > 1:4060162/1‑70 (MQ=255) aTTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCAt > 1:546633/1‑70 (MQ=255) tttCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAGGTGGGTGGTGAc < 1:2905679/66‑1 (MQ=255) tttCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGAcc < 1:4065994/67‑1 (MQ=255) tttCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCa > 1:5628321/1‑71 (MQ=255) tCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCAcc > 1:3003380/1‑71 (MQ=255) tCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCAcc > 1:4808392/1‑71 (MQ=255) ccGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGt < 1:945889/71‑1 (MQ=255) ccGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGt < 1:4330453/71‑1 (MQ=255) ccGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGt < 1:3236423/71‑1 (MQ=255) ccGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGt < 1:1008892/71‑1 (MQ=255) gcgGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCAt > 1:4346371/1‑63 (MQ=255) gcgGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCAt > 1:774054/1‑63 (MQ=255) gcgGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCACCCATAAAGTGggtggt > 1:3178283/1‑57 (MQ=255) cgGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGAc < 1:3289606/71‑1 (MQ=255) cgGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGAc < 1:3780911/71‑1 (MQ=255) tACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGcc < 1:162026/71‑1 (MQ=255) tACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGcc < 1:2587750/71‑1 (MQ=255) cacCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATc < 1:2085227/58‑1 (MQ=255) acCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCttt < 1:1976867/71‑1 (MQ=255) acCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCttt < 1:2252577/71‑1 (MQ=255) cAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTtct > 1:2367174/1‑71 (MQ=255) aGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAgtgggt > 1:2262723/1‑44 (MQ=255) aGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTtctc > 1:1463739/1‑71 (MQ=255) aGGCCGATGCGGTCGCAATATTCC‑CTTCATCCATaa < 1:4243377/36‑1 (MQ=38) ggCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGt > 1:2096646/1‑58 (MQ=255) cGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACg > 1:4365044/1‑59 (MQ=255) gATGCGGTCGCAACGTTCCGCTTCGTTCATAAAgtggg > 1:4929905/1‑38 (MQ=21) gATGCGGTCGCAACGTTCCGCTTCGTTCATAAAgtggg > 1:4480777/1‑38 (MQ=21) aTGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGt < 1:3458725/53‑1 (MQ=255) aTGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGt < 1:1641699/53‑1 (MQ=255) tGCGGTCGCAACGTTCCGCTTCGTTCATAAAGTGGGTGgagatg < 1:5382951/44‑3 (MQ=19) tGCGGTCGCAACGTTCCGCTTCGTTCATAAAGTGGGTGgagatg < 1:2428844/44‑3 (MQ=19) gCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGa > 1:4748028/1‑53 (MQ=255) gCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGc > 1:3752177/1‑71 (MQ=255) gTCGCAATATTCCGCTTCATCCATAAAgtgggtgg > 1:4195918/1‑35 (MQ=255) gTCGCAATATTCCGCTTCATCCATAAAgtgggtgg > 1:3287737/1‑35 (MQ=255) gTCGCAATATTCCGCTTCATCCATAAAgtgggtgg > 1:752131/1‑35 (MQ=255) gTCGCAATATTCCGCTTCATCCATAAAgtgggtgg > 1:2139921/1‑35 (MQ=255) tCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGtt < 1:1465206/71‑1 (MQ=255) tCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGtt < 1:1727380/71‑1 (MQ=255) tCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGtt > 1:256481/1‑71 (MQ=255) cGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGtt > 1:2831203/1‑70 (MQ=255) cGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGtt > 1:3238625/1‑70 (MQ=255) gCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTg < 1:2869320/70‑1 (MQ=255) gCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTg < 1:5446902/70‑1 (MQ=255) gCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGa < 1:4395727/71‑1 (MQ=255) aaaTATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGAc < 1:641677/46‑1 (MQ=255) tatTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGAtgtg < 1:1016677/71‑1 (MQ=255) | ATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTG > minE/533802‑533933 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |